Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,639,576	(A)6→5	coding (145/336 nt)	uspB ←	putative universal stress (ethanol tolerance) protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,639,571	0	A	.	100.0%	340.8 / NA	78	coding (150/336 nt)	uspB	putative universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (38/40);  total (38/40)

CACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATG  >  NC_000913/3639431‑3639704                                                                                                                                             |                                                                                                                                      cacaATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGAT‑ACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTa                                                                                                                                       >  2:550815/1‑140 (MQ=255)    aaTGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaa                                                                                                                                     >  2:388211/1‑140 (MQ=255)          cTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagccc                                                                                                                               <  1:264725/140‑5 (MQ=255)           tGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGcccc                                                                                                                             <  1:882217/140‑1 (MQ=255)           tGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGcccc                                                                                                                             >  2:400561/1‑140 (MQ=255)           tGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGcccc                                                                                                                             <  2:376819/140‑1 (MQ=255)            ggTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGccccc                                                                                                                            <  1:514664/140‑1 (MQ=255)            ggTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGccccc                                                                                                                            >  2:83759/1‑140 (MQ=255)             gTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGccccc                                                                                                                            >  1:1189601/1‑139 (MQ=255)             gTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGccccc                                                                                                                            <  2:1189601/139‑1 (MQ=255)                aGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCa                                                                                                                        >  1:200648/1‑140 (MQ=255)                  aaTAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATc                                                                                                                      <  1:245132/140‑1 (MQ=255)                  aaTAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATc                                                                                                                      <  1:1110343/140‑1 (MQ=255)                    tAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCaa                                                                                                                    <  2:200648/140‑1 (MQ=255)                      aaCTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACa                                                                                                                  <  2:619185/140‑1 (MQ=255)                       aCTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACat                                                                                                                 <  1:665646/140‑1 (MQ=255)                            cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            >  2:740216/1‑140 (MQ=255)                            cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            <  1:144864/140‑1 (MQ=255)                            cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            >  1:1168560/1‑140 (MQ=255)                            cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            >  2:502726/1‑140 (MQ=255)                            cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            >  2:1095947/1‑140 (MQ=255)                              aCGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATa                                                                                                          >  2:168756/1‑140 (MQ=255)                                   cccGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCa                                                                                                     <  2:609672/140‑1 (MQ=255)                                     cGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAAt                                                                                                   >  1:218378/1‑140 (MQ=255)                                               ggcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGt                                                                                         <  1:740216/140‑1 (MQ=255)                                                 cgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAg                                                                                          <  1:804330/137‑1 (MQ=255)                                                 cgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAg                                                                                          >  2:804330/1‑137 (MQ=255)                                                  gAATAAATTCATCATCGTGATGATCGCGAT‑ACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg                                                                                     <  1:550815/140‑1 (MQ=255)                                                   aaTAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg                                                                                     <  1:866890/140‑1 (MQ=255)                                                     tAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCa                                                                                   <  1:388211/140‑1 (MQ=255)                                                     tAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCa                                                                                   <  1:672092/140‑1 (MQ=255)                                                       aaTTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATGGAGCAATGGATCGCAGTTACGCAGt                                                                                 <  1:1054786/140‑1 (MQ=255)                                                       aaTTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGt                                                                                 <  1:90333/140‑1 (MQ=255)                                                                tcGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaac                                                                        <  1:445580/140‑1 (MQ=255)                                                                 cGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaaca                                                                       <  1:420834/140‑1 (MQ=255)                                                                 cGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaaca                                                                       >  2:856680/1‑140 (MQ=255)                                                                   tgatgaTCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaacaac                                                                     >  2:910618/1‑140 (MQ=255)                                                                     atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg                                                                                     >  2:865419/1‑122 (MQ=255)                                                                     atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg                                                                                     <  1:865419/122‑1 (MQ=255)                                                                     atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgc                                                                   >  2:317670/1‑140 (MQ=255)                                                                        atCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcgcg                                                                <  2:1168560/140‑1 (MQ=255)                                                                          cgcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTa                                                              >  2:702145/1‑140 (MQ=255)                                                                           gcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAg                                                             <  1:168756/140‑1 (MQ=255)                                                                           gcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAg                                                             <  2:218378/140‑1 (MQ=255)                                                                            cgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGt                                                            >  2:1012942/1‑140 (MQ=255)                                                                               tAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgat                                                         <  1:709801/140‑1 (MQ=255)                                                                                       ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcg                                                                  >  1:309413/1‑123 (MQ=255)                                                                                       ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcg                                                                  <  2:309413/123‑1 (MQ=255)                                                                                           tAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc                                             <  1:1012942/140‑1 (MQ=255)                                                                                           tAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc                                             <  1:718820/140‑1 (MQ=255)                                                                                            aGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcg                                            >  2:959673/1‑140 (MQ=255)                                                                                             gATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc                                           <  1:1072521/140‑1 (MQ=255)                                                                                             gATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc                                           <  1:910618/140‑1 (MQ=255)                                                                                              atatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc                                             >  2:1071068/1‑137 (MQ=255)                                                                                              atatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc                                             <  1:1071068/137‑1 (MQ=255)                                                                                              atatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGcc                                          <  2:1041868/140‑1 (MQ=255)                                                                                                ataCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCAt                                        <  1:375857/140‑1 (MQ=255)                                                                                                 taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCa                                         <  2:759887/138‑1 (MQ=255)                                                                                                 taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCa                                         >  1:759887/1‑138 (MQ=255)                                                                                                 taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATg                                       >  1:530124/1‑140 (MQ=255)                                                                                                 taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATg                                       >  1:332319/1‑140 (MQ=255)                                                                                                  aCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGt                                      >  2:1105919/1‑140 (MQ=255)                                                                                                  aCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGt                                      >  2:197774/1‑140 (MQ=255)                                                                                                   ccAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGtt                                     >  2:1189950/1‑140 (MQ=255)                                                                                                      aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAAc                                  >  1:904458/1‑140 (MQ=255)                                                                                                      aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAAc                                  >  1:324843/1‑140 (MQ=255)                                                                                                      aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAAc                                  <  1:702145/140‑1 (MQ=255)                                                                                                      aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAAc                                  >  2:752252/1‑140 (MQ=255)                                                                                                       aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa                                 >  1:36444/1‑140 (MQ=255)                                                                                                       aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa                                 >  1:601365/1‑140 (MQ=255)                                                                                                       aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa                                 >  1:676156/1‑140 (MQ=255)                                                                                                       aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa                                 >  1:140766/1‑140 (MQ=255)                                                                                                        cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc                                           <  1:444780/129‑1 (MQ=255)                                                                                                        cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc                                           >  2:444780/1‑129 (MQ=255)                                                                                                        cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACaa                                >  1:1215472/1‑140 (MQ=255)                                                                                                           gaCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGc                             >  2:999674/1‑140 (MQ=255)                                                                                                              gCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCaaa                          <  1:1095947/140‑1 (MQ=255)                                                                                                                          tgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAg              >  2:369150/1‑140 (MQ=255)                                                                                                                            ggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGcc            <  1:502726/140‑1 (MQ=255)                                                                                                                              ttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCa          <  1:317670/140‑1 (MQ=255)                                                                                                                                      gtgAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATg  >  2:671138/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                      CACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATG  >  NC_000913/3639431‑3639704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑