Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,485,103	(G)5→4	coding (2043/3903 nt)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,485,099	0	G	.	100.0%	246.6 / NA	57	coding (2039/3903 nt)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (31/26);  total (31/26)

ATGAAAGATGCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGT  >  NC_000913/1484966‑1485231                                                                                                                                      |                                                                                                                                     aTGAAAGATGCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTggggcgc                                                                                                                                <  1:1034638/140‑4 (MQ=255)     aaGATGCCGATAAACAAGAATATATCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATtg                                                                                                                           >  1:538185/1‑140 (MQ=255)     aaGATGCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATtg                                                                                                                           <  1:582002/140‑1 (MQ=255)          gCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATtgct                                                                                                                         <  1:541321/137‑1 (MQ=255)          gCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATtgct                                                                                                                         >  2:541321/1‑137 (MQ=255)                  aCAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGa                                                                                                              >  1:417459/1‑140 (MQ=255)                   cAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTAT‑GGGGCGCATTGCTGCGCGTATCGAc                                                                                                             <  2:322699/140‑1 (MQ=255)                           taCCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATg                                                                                                     >  2:347258/1‑140 (MQ=255)                            aCCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATgg                                                                                                    >  2:543044/1‑140 (MQ=255)                             ccGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATggg                                                                                                   <  2:277888/140‑1 (MQ=255)                                gcgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGaa                                                                                                       <  1:418593/133‑1 (MQ=255)                                gcgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGaa                                                                                                       >  2:418593/1‑133 (MQ=255)                                 cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTAt                                                                                                                 >  2:103739/1‑122 (MQ=255)                                 cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTAt                                                                                                                 <  1:103739/122‑1 (MQ=255)                                 cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGaa                                                                                                       <  2:626813/132‑1 (MQ=255)                                 cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGaa                                                                                                       >  1:626813/1‑132 (MQ=255)                                 cgcACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGa                                                                                               >  1:500268/1‑140 (MQ=255)                                   cACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGc                                                                                             >  2:292427/1‑140 (MQ=255)                                      gTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAg                                                                                          <  2:500268/140‑1 (MQ=255)                                           gcgcgTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     <  1:1031915/140‑1 (MQ=255)                                                tttCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCa                                                                                <  2:338124/140‑1 (MQ=255)                                                tttCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCa                                                                                >  2:1075396/1‑140 (MQ=255)                                                tttCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCa                                                                                <  1:825877/140‑1 (MQ=255)                                                       aTCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGAtt                                                                         >  2:730673/1‑140 (MQ=255)                                                         cTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTaa                                                                       >  1:322905/1‑140 (MQ=255)                                                         cTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTaa                                                                       >  2:302070/1‑140 (MQ=255)                                                          ttCCCCGGTTTTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTaaa                                                                      <  1:456024/140‑1 (MQ=255)                                                          ttCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTaaa                                                                      <  1:730673/140‑1 (MQ=255)                                                           tCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     <  1:1061167/124‑1 (MQ=255)                                                           tCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     >  2:1061167/1‑124 (MQ=255)                                                           tCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAAc                                                                     >  1:548426/1‑140 (MQ=255)                                                            gcgcGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACg                                                                    <  1:567134/137‑1 (MQ=255)                                                             cccGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     >  2:1186429/1‑122 (MQ=255)                                                             cccGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCt                                                                                     <  1:1186429/122‑1 (MQ=255)                                                             cccGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGc                                                                   >  1:1113738/1‑140 (MQ=255)                                                                ggTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCAcc                                                                >  2:865968/1‑140 (MQ=255)                                                                   tCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTAc                                                             >  2:1041836/1‑140 (MQ=255)                                                                    cTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACa                                                            >  1:412516/1‑140 (MQ=255)                                                                         ttATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGaa                                                       <  1:1075396/140‑1 (MQ=255)                                                                          tATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAAc                                                      <  1:390848/140‑1 (MQ=255)                                                                            ttCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCg                                                    >  1:882724/1‑140 (MQ=255)                                                                                     ccGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGaa                                           <  1:347258/140‑1 (MQ=255)                                                                                             aTGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCa                                   <  1:543044/140‑1 (MQ=255)                                                                                                ggTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAggg                                >  1:1190599/1‑140 (MQ=255)                                                                                                  tAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGgcg                              <  1:645245/140‑1 (MQ=255)                                                                                                   aaTGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGgc                               >  2:1195973/1‑138 (MQ=255)                                                                                                   aaTGGTGGCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGgc                               <  1:1195973/138‑1 (MQ=255)                                                                                                       gtggCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTg                         >  2:463086/1‑140 (MQ=255)                                                                                                          gCGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATg                      >  1:1191590/1‑140 (MQ=255)                                                                                                           cGGAACTGGTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATgg                     >  2:1188791/1‑140 (MQ=255)                                                                                                                  ggTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGa              <  2:412516/140‑1 (MQ=255)                                                                                                                  ggTAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGa              <  1:1188791/140‑1 (MQ=255)                                                                                                                    tAGAAACCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGaaa            >  1:163086/1‑140 (MQ=255)                                                                                                                         aCCAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTc       >  2:351927/1‑140 (MQ=255)                                                                                                                          ccAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCa      <  1:865968/140‑1 (MQ=255)                                                                                                                          ccAGCCGCCTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCa      <  1:123818/140‑1 (MQ=255)                                                                                                                             gccgccTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTg   >  1:702879/1‑140 (MQ=255)                                                                                                                              ccgccTGT‑GGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGt  >  1:970754/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     ATGAAAGATGCCGATAAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGT  >  NC_000913/1484966‑1485231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑