Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	367,852	(GC)6→5	coding (568‑569/834 nt)	mhpR ←	DNA‑binding transcriptional activator MhpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	367,842	0	G	.	100.0%	256.0 / NA	59	coding (579/834 nt)	mhpR	DNA‑binding transcriptional activator MhpR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (32/27);  total (32/27)
*	NC_000913	367,843	0	C	.	100.0%	256.0 / NA	59	coding (578/834 nt)	mhpR	DNA‑binding transcriptional activator MhpR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (32/27);  total (32/27)

TTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAG  >  NC_000913/367704‑367973                                                                                                                                           ||                                                                                                                                   ttCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgc                                                                                                                                    >  1:1106600/1‑140 (MQ=255)    aaTGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcg                                                                                                                                 <  2:860062/140‑1 (MQ=255)       ggTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgc                                                                                                                              <  1:35120/140‑1 (MQ=255)         tCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgc                                                                                                                            >  1:1194145/1‑140 (MQ=255)           aTTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgcca                                                                                                                          >  2:74669/1‑138 (MQ=255)             tGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcg                                                                                                                                   <  2:27209/129‑1 (MQ=255)             tGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcg                                                                                                                                   >  1:27209/1‑129 (MQ=255)                  tCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGc                                                                                                                 >  2:860923/1‑140 (MQ=255)                     ccATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTc                                                                                                              <  2:606030/140‑1 (MQ=255)                              ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTaa                                                                                                     >  2:1036484/1‑140 (MQ=255)                              ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTaa                                                                                                     >  2:807565/1‑140 (MQ=255)                              ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTaa                                                                                                     >  1:316414/1‑140 (MQ=255)                               cAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                                                                    <  2:41140/140‑1 (MQ=255)                               cAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                                                                    >  1:665488/1‑140 (MQ=255)                               cAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                                                                    >  2:561668/1‑140 (MQ=255)                               cAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                                                                    >  1:11140/1‑140 (MQ=255)                                aGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTc                                                                                                              >  2:1168224/1‑129 (MQ=255)                                aGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTc                                                                                                              <  1:1168224/129‑1 (MQ=255)                                aGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACg                                                                                                   >  1:615537/1‑140 (MQ=255)                                  aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGt                                                                                                 >  1:180307/1‑140 (MQ=255)                                  aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGt                                                                                                 >  1:922553/1‑140 (MQ=255)                                  aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGt                                                                                                 >  1:472823/1‑140 (MQ=255)                                  aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGt                                                                                                 >  1:688702/1‑140 (MQ=255)                                  aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGt                                                                                                 >  2:146197/1‑140 (MQ=255)                                       gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCg                                                                                            >  1:1042149/1‑140 (MQ=255)                                       gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCg                                                                                            <  1:74669/140‑1 (MQ=255)                                       gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCg                                                                                            >  2:147311/1‑140 (MQ=255)                                         cAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                                                                          <  1:561668/140‑1 (MQ=255)                                         cAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                                                                          <  2:1106600/140‑1 (MQ=255)                                         cAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                                                                          <  1:97377/140‑1 (MQ=255)                                         cAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                                                                          >  2:591219/1‑140 (MQ=255)                                          aGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                                                                         <  2:316414/140‑1 (MQ=255)                                              aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                                                                     <  2:1194145/140‑1 (MQ=255)                                              aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                                                                     <  2:982006/140‑1 (MQ=255)                                                 cACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGa                                                                                  <  1:3933/140‑1 (MQ=255)                                                 cACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGa                                                                                  <  1:591219/140‑1 (MQ=255)                                                        tgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcat                                                                           >  1:667642/1‑140 (MQ=255)                                                         gttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                                                                          >  2:894460/1‑140 (MQ=255)                                                           tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAc                                                                        <  2:1042149/140‑1 (MQ=255)                                                                 cgcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGc                                                                  >  2:1123226/1‑140 (MQ=255)                                                                  gcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                                                                 >  1:935711/1‑140 (MQ=255)                                                                   cAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                                                                <  1:807565/140‑1 (MQ=255)                                                                   cAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                                                                <  2:180307/140‑1 (MQ=255)                                                                     gCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa                                                              <  2:635966/140‑1 (MQ=255)                                                                      cGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg                                                             <  1:1123226/140‑1 (MQ=255)                                                                         tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCt                                                          <  1:1036484/140‑1 (MQ=255)                                                                               gATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGTGAGGCTAACAtt                                                    <  1:146197/140‑1 (MQ=255)                                                                               gATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAtt                                                    <  1:147311/140‑1 (MQ=255)                                                                                   gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCgg                                                >  2:745080/1‑139 (MQ=255)                                                                                     ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                                                                >  2:400176/1‑122 (MQ=255)                                                                                     ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                                                                <  1:400176/122‑1 (MQ=255)                                                                                              ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTg                                     <  2:688702/140‑1 (MQ=255)                                                                                              ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTg                                     <  2:935711/140‑1 (MQ=255)                                                                                                    ctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCt                               >  1:331357/1‑140 (MQ=255)                                                                                                    ctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCt                               >  2:198691/1‑140 (MQ=255)                                                                                                      gATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTcc                                        >  2:67947/1‑129 (MQ=255)                                                                                                      gATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTcc                                        <  1:67947/129‑1 (MQ=255)                                                                                                       aTCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTg                                     <  1:572293/131‑1 (MQ=255)                                                                                                       aTCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTg                                     >  2:572293/1‑131 (MQ=255)                                                                                                              ccGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGc                     >  1:832364/1‑140 (MQ=255)                                                                                                              ccGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGc                     >  2:1034350/1‑140 (MQ=255)                                                                                                                  ggTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCaaaa                 >  1:162596/1‑140 (MQ=255)                                                                                                                         cTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagc          <  2:615537/140‑1 (MQ=255)                                                                                                                          tGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc         <  2:832364/140‑1 (MQ=255)                                                                                                                            tCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGccagccag       >  1:31422/1‑140 (MQ=255)                                                                                                                                 aaCCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAg  >  2:196739/1‑140 (MQ=255)                                                                                                                                           ||                                                                                                                                   TTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAG  >  NC_000913/367704‑367973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑