Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,432,994	(C)6→7	coding (567/1125 nt)	smf ←	protein Smf

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,432,988	1	.	C	95.2%	295.9 / 6.2	84	coding (573/1125 nt)	smf	protein Smf
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base C (39/41);  total (40/44)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

GTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACACCATC  >  NC_000913/3432849‑3433116                                                                                                                                             |                                                                                                                                   gTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg                                                                                                                                     <  1:947785/140‑1 (MQ=255)  tGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg‑c                                                                                                                                   >  2:1041426/1‑140 (MQ=255)   gCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑cc                                                                                                                                  >  2:992606/1‑140 (MQ=255)      gTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑ccc‑c‑c                                                                                                                             <  1:911594/140‑1 (MQ=255)      gTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑ccc‑c‑c                                                                                                                             <  1:411008/140‑1 (MQ=255)        cACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑ccc‑c‑ccc                                                                                                                           <  2:1109247/140‑2 (MQ=255)         aCCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑ccc‑c‑ccct                                                                                                                          >  2:969526/1‑138 (MQ=255)           cAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑ccc‑c‑ccctgt                                                                                                                        >  1:134307/1‑136 (MQ=255)             gCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTc                                                                                                                       <  1:775212/140‑1 (MQ=255)                 aCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGc                                                                                                                   >  1:656300/1‑140 (MQ=255)                  ccACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCa                                                                                                                  <  1:309451/140‑1 (MQ=255)                   cACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAg                                                                                                                 >  2:222129/1‑140 (MQ=255)                     cTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGAc                                                                                                               >  1:569356/1‑140 (MQ=255)                        cGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTgg                                                                                                            >  2:662530/1‑140 (MQ=255)                        cGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTGCGTGGGAAATTGTAAGCAAGGAGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCCAC‑CCTGTCCATGCAGACTgg                                                                                                            >  2:609586/1‑140 (MQ=255)                           aaaGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAg                                                                                                         >  2:86788/1‑140 (MQ=255)                             aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGcc                                                                                                       >  1:1091947/1‑140 (MQ=255)                             aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGcc                                                                                                       >  2:184793/1‑140 (MQ=255)                             aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGcc                                                                                                       >  2:1155229/1‑140 (MQ=255)                             aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCC‑C‑CCTGTTCAAGCAGACTGGCAGCCa                                                                                                      >  2:202133/1‑140 (MQ=255)                               ccGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAg                                                                                                     <  1:609586/140‑1 (MQ=255)                               ccGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAg                                                                                                     >  2:599554/1‑140 (MQ=255)                                cGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGt                                                                                                    >  1:482969/1‑140 (MQ=255)                                cGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGt                                                                                                    <  2:737564/140‑1 (MQ=255)                                cGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGAAGCCAGt                                                                                                    >  1:886345/1‑140 (MQ=255)                                       accaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCAt                                                                                             <  1:1155229/140‑1 (MQ=255)                                         caccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGa                                                                                           <  1:19441/140‑1 (MQ=255)                                           ccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcg                                                                                         <  2:704126/140‑1 (MQ=255)                                            caGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg                                                                                        <  1:184793/140‑1 (MQ=255)                                              gTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg                                                                                      <  1:662530/140‑1 (MQ=255)                                               tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcgg                                                                                     >  1:671913/1‑140 (MQ=255)                                                acacCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCggg                                                                                    >  2:523266/1‑140 (MQ=255)                                                   ccTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt                                                                                 >  1:1022172/1‑140 (MQ=255)                                                   ccTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt                                                                                 <  2:656300/140‑1 (MQ=255)                                                    cTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATg                                                                                >  1:90726/1‑140 (MQ=255)                                                     tttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                               >  2:396908/1‑140 (MQ=255)                                                      ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa                                                                              <  1:1041426/140‑1 (MQ=255)                                                       tACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                             <  1:992606/140‑1 (MQ=255)                                                       tACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                             <  2:90726/140‑1 (MQ=255)                                                       tACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGAAGCCAGTCGGGCATGACGGCGGGGATGAAt                                                                             <  2:886345/140‑1 (MQ=255)                                                              aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                               >  1:395431/1‑131 (MQ=255)                                                              aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa                                                                               <  2:395431/131‑1 (MQ=255)                                                              aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTa                                                                      >  2:298379/1‑140 (MQ=255)                                                              aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTa                                                                      >  2:113400/1‑140 (MQ=255)                                                              aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTa                                                                      >  2:1111675/1‑140 (MQ=255)                                                              aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTa                                                                      <  1:113400/140‑1 (MQ=255)                                                                 aCTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGt                                                                   >  2:220115/1‑140 (MQ=255)                                                                 aCTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGt                                                                   >  2:377201/1‑140 (MQ=255)                                                                  cTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                                  >  1:706977/1‑140 (MQ=255)                                                                    gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                                <  1:1169413/140‑1 (MQ=255)                                                                    gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                                <  2:1091947/140‑1 (MQ=255)                                                                    gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                                <  2:482969/140‑1 (MQ=255)                                                                    gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                                <  1:826969/140‑1 (MQ=255)                                                                      tAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCAtt                                                              >  2:1226288/1‑140 (MQ=255)                                                                       aaTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                             <  1:519626/140‑1 (MQ=255)                                                                       aaTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                             <  1:86788/140‑1 (MQ=255)                                                                       aaTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                             <  1:298379/140‑1 (MQ=255)                                                                        aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGTGATGAATGGTATTAAGTCCATTcc                                                            >  1:983393/1‑140 (MQ=255)                                                                         tGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTaa                                                                     >  1:180550/1‑130 (MQ=255)                                                                         tGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTaa                                                                     <  2:180550/130‑1 (MQ=255)                                                                         tGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                           <  2:1022172/140‑1 (MQ=255)                                                                          gCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTcccc                                                          >  2:252825/1‑140 (MQ=255)                                                                            gATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa                                                        <  2:134307/140‑1 (MQ=255)                                                                             aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                           <  1:451630/136‑1 (MQ=255)                                                                             aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                           >  1:342426/1‑136 (MQ=255)                                                                             aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                           <  2:342426/136‑1 (MQ=255)                                                                             aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                           >  2:451630/1‑136 (MQ=255)                                                                             aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAAt                                                       <  1:969526/140‑1 (MQ=255)                                                                              tttCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATa                                                      <  1:523266/140‑1 (MQ=255)                                                                               ttcttcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCAAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATAc                                                     >  2:1104033/1‑140 (MQ=255)                                                                                tcttcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACa                                                    >  1:1143106/1‑140 (MQ=255)                                                                                   tcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCa                                                 <  1:178938/140‑1 (MQ=255)                                                                                            aTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg                                        <  2:569356/140‑1 (MQ=255)                                                                                             ttGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGc                                       <  2:37200/140‑1 (MQ=255)                                                                                              tGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCACAATACAGCAATGCTGACGCCa                                     <  1:202133/140‑1 (MQ=255)                                                                                               gTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCa                                     <  1:599554/140‑1 (MQ=255)                                                                                                 aaGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCAtt                                   <  2:706977/140‑1 (MQ=255)                                                                                                      aGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTg                             <  1:597110/140‑1 (MQ=255)                                                                                                       ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTg                             >  1:564941/1‑140 (MQ=255)                                                                                                       ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCC‑CACCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTg                             <  2:1143106/140‑1 (MQ=255)                                                                                                             gAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGgc                       <  1:103434/140‑1 (MQ=255)                                                                                                                  tCGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCtt                  >  1:1162028/1‑140 (MQ=255)                                                                                                                   cGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCttt                 >  2:1163359/1‑140 (MQ=255)                                                                                                                   cGAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGACCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCttt                 >  1:68393/1‑140 (MQ=255)                                                                                                                    gAGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTa                <  1:1163359/140‑1 (MQ=255)                                                                                                                     aGGGGAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTAt               >  2:878320/1‑140 (MQ=255)                                                                                                                        ggAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATgcg            >  1:250264/1‑140 (MQ=255)                                                                                                                        ggAAATTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAGGCAAGGCTATAAACCCCTTCCCCCATACACCAATTATCCCGACATTTACCTGTAAGGGTGCCTTATgcg            >  2:1048350/1‑140 (MQ=255)                                                                                                                          aaaTTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCt          <  1:252825/140‑1 (MQ=255)                                                                                                                           aaTTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTa         <  2:1162028/140‑1 (MQ=255)                                                                                                                            aTTCCGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTac        <  1:396908/140‑1 (MQ=255)                                                                                                                               ccGAGACGAGAGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACAcc     <  1:1226288/140‑1 (MQ=255)                                                                                                                                  agacgagaGCGCCCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACACCATc  >  2:634744/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                   GTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCG‑CCC‑C‑CCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACACCATC  >  NC_000913/3432849‑3433116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑