Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	467,823	(A)7→8	coding (412/819 nt)	cof →	HMP‑PP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	467,816	1	.	A	100.0%	183.2 / NA	51	V135V (GTC→GTA)	cof	HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (24/27);  total (24/27)

TGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC‑AAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTG  >  NC_000913/467680‑467947                                                                                                                                          |                                                                                                                                    tGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC‑aaa                                                                                                                                  <  1:609676/140‑1 (MQ=255)  ggTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC‑aaaa                                                                                                                                 >  1:134931/1‑140 (MQ=255)      cTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC‑aaaaaaaa                                                                                                                             <  1:769063/140‑2 (MQ=255)           tCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCCGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCa                                                                                                                         <  2:741133/140‑1 (MQ=255)               cAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCg                                                                                                                     >  1:1137136/1‑140 (MQ=255)               cAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCg                                                                                                                     <  2:590912/140‑1 (MQ=255)                aaTGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCgg                                                                                                                    <  2:134931/140‑1 (MQ=255)                   gggATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAg                                                                                                                 >  1:313849/1‑140 (MQ=255)                   gggATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAg                                                                                                                 >  1:791015/1‑140 (MQ=255)                   gggATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAg                                                                                                                 >  1:1077094/1‑140 (MQ=255)                   gggATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAg                                                                                                                 >  1:548296/1‑140 (MQ=255)                   gggATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAg                                                                                                                 >  1:697481/1‑140 (MQ=255)                    ggATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGc                                                                                                                <  2:1077094/140‑1 (MQ=255)                      aTACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCCATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGt                                                                                                              <  2:668434/140‑1 (MQ=255)                        aCCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCa                                                                                                            <  2:878834/140‑1 (MQ=255)                         cccGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCAc                                                                                                           <  2:697481/140‑1 (MQ=255)                           cGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCa                                                                                                         <  2:791015/140‑1 (MQ=255)                            gAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCaa                                                                                                        >  1:497453/1‑140 (MQ=255)                              gCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGa                                                                                                      >  1:545051/1‑140 (MQ=255)                               ccAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGAt                                                                                                     >  1:111137/1‑140 (MQ=255)                                cAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATc                                                                                                    <  2:911775/140‑1 (MQ=255)                                    atgcatATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCg                                                                                                               >  1:925975/1‑125 (MQ=255)                                    atgcatATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCg                                                                                                               <  2:925975/125‑1 (MQ=255)                                     tgcatATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCtt                                                                                               <  2:195018/140‑1 (MQ=255)                                         tatCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgt                                                                                           <  2:532125/140‑1 (MQ=255)                                                aaTGACGACGGTTGGTTTACCGGGAAAGAGATCCTTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgtg                                                                                          >  1:341122/1‑134 (MQ=255)                                                aaTGACGACGGTTGGTTTACCGGGAAAGAGATCCTTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgtg                                                                                          <  2:341122/134‑1 (MQ=255)                                                aaTGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc                                                                                    >  1:536855/1‑140 (MQ=255)                                                aaTGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc                                                                                    >  1:294192/1‑140 (MQ=255)                                                aaTGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc                                                                                    >  1:995764/1‑140 (MQ=255)                                                  tGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCac                                                                                  >  1:125663/1‑140 (MQ=255)                                                    acgacgGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCacga                                                                                >  1:1217950/1‑140 (MQ=255)                                                    acgacgGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCacga                                                                                <  2:294192/140‑1 (MQ=255)                                                      gacgGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCacgacg                                                                              <  2:548296/140‑1 (MQ=255)                                                      gacgGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCacgacg                                                                              <  2:1217950/140‑1 (MQ=255)                                                       acgGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGa                                                                                      <  2:94846/131‑1 (MQ=255)                                                       acgGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGa                                                                                      >  1:94846/1‑131 (MQ=255)                                                        cgGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGAt                                                                            >  1:233516/1‑140 (MQ=255)                                                              gttTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTaca                                                                      <  2:233516/140‑1 (MQ=255)                                                                ttACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTCTAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACg                                                                    <  2:1137136/140‑1 (MQ=255)                                                                          agagATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATc                                                          <  2:313849/140‑1 (MQ=255)                                                                             gaTCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAg                                                       <  2:536855/140‑1 (MQ=255)                                                                                              aGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAgg                                      <  2:1172117/140‑1 (MQ=255)                                                                                              aGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAgg                                      <  2:497453/140‑1 (MQ=255)                                                                                                 cATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGa                                   <  2:545051/140‑1 (MQ=255)                                                                                                     tGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGt                               <  2:396099/140‑1 (MQ=255)                                                                                                       tCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGc                             <  2:111137/140‑1 (MQ=255)                                                                                                              cGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTtg                      >  2:562421/1‑140 (MQ=255)                                                                                                              cGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTtg                      >  1:211569/1‑140 (MQ=255)                                                                                                              cGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTtg                      >  1:1198643/1‑140 (MQ=255)                                                                                                                gTTTTCGTTATCAGATAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTtgtg                    >  1:297445/1‑140 (MQ=255)                                                                                                                              aTAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACgg      >  1:1183183/1‑140 (MQ=255)                                                                                                                               tAATCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGa     <  2:1198643/140‑1 (MQ=255)                                                                                                                                  tCGATGTCAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTg  >  2:334049/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    TGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC‑AAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTG  >  NC_000913/467680‑467947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑