Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,440,941	(A)7→8	intergenic (‑157/‑117)	ydbK ← / → ydbJ	putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,440,934	1	.	A	100.0%	153.3 / NA	44	intergenic (‑150/‑124)	ydbK/ydbJ	putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (19/25);  total (19/25)

CATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑AAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGAGCAGC  >  NC_000913/1440797‑1441068                                                                                                                                           |                                                                                                                                       cATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑aa                                                                                                                                      <  1:1021592/140‑1 (MQ=255)      cgcAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑aaaaaaa                                                                                                                                 >  2:209994/1‑140 (MQ=255)      cgcAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑aaaaaaa                                                                                                                                 >  2:1125990/1‑140 (MQ=255)      cgcAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑a                                                                                                                                       >  2:43194/1‑134 (MQ=255)      cgcAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑a                                                                                                                                       <  1:43194/134‑1 (MQ=255)                gggCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATaa                                                                                                                        >  1:1052637/1‑140 (MQ=255)                     cACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAg                                                                                                                   >  1:1019651/1‑140 (MQ=255)                                    cgcCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATg                                                                                                           >  1:10099/1‑133 (MQ=255)                                    cgcCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATg                                                                                                           <  2:10099/133‑1 (MQ=255)                                    cgcCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCa                                                                                                    <  1:209994/140‑1 (MQ=255)                                    cgcCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCa                                                                                                    >  1:713834/1‑140 (MQ=255)                                         agtagtAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAAcgcg                                                                                               <  2:181458/140‑1 (MQ=255)                                            agtaATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAg                                                                                            <  2:969780/140‑1 (MQ=255)                                                aTCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACa                                                                                        <  2:374730/140‑1 (MQ=255)                                                 tCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGAAAAAATGCGGGGCAACGCGCAGGACAg                                                                                       >  1:586713/1‑140 (MQ=255)                                                     tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  2:611314/1‑140 (MQ=255)                                                     tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  1:67915/1‑140 (MQ=255)                                                     tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  1:1087568/1‑140 (MQ=255)                                                     tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  2:1159368/1‑140 (MQ=255)                                                     tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   >  2:855413/1‑140 (MQ=255)                                                     tCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGctct                                                                                   <  1:20428/140‑1 (MQ=255)                                                        aTTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGa                                                                                >  1:1129913/1‑140 (MQ=255)                                                          tttAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGAACAGCTCTCGAcg                                                                              <  2:636048/140‑1 (MQ=255)                                                            tAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGAcgcc                                                                            <  2:586713/140‑1 (MQ=255)                                                            tAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGAcgcc                                                                            <  2:1056043/140‑1 (MQ=255)                                                               cAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACgccgcc                                                                         <  2:1019651/140‑1 (MQ=255)                                                                aaaTGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGccc                                                                        <  1:856273/140‑1 (MQ=255)                                                                 aaTGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCct                                                                       <  1:953045/140‑1 (MQ=255)                                                                   tGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCctct                                                                     >  2:51229/1‑140 (MQ=255)                                                                      cTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCtgtg                                                                  <  1:51229/140‑1 (MQ=255)                                                                        ttcttcTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGtgct                                                                <  2:67915/140‑1 (MQ=255)                                                                          cttctGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGtgctgc                                                              <  2:1129913/140‑1 (MQ=255)                                                                          cttctGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGtgctgc                                                              <  1:855413/140‑1 (MQ=255)                                                                            tctGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCt                                                            >  2:812498/1‑140 (MQ=255)                                                                             ctGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCta                                                           <  2:439410/140‑1 (MQ=255)                                                                               gCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCtatt                                                         <  2:1058691/140‑1 (MQ=255)                                                                                          tttGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCgg                                              >  1:946856/1‑140 (MQ=255)                                                                                                 cccccACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGtt                                       >  2:949076/1‑140 (MQ=255)                                                                                                 cccccACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGtt                                       <  2:49282/140‑1 (MQ=255)                                                                                                       caTCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCtt                                 >  2:266682/1‑140 (MQ=255)                                                                                                       caTCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCtt                                 >  2:1210119/1‑140 (MQ=255)                                                                                                        aTCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTg                                <  1:1210119/140‑1 (MQ=255)                                                                                                        aTCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTg                                <  2:1052637/140‑1 (MQ=255)                                                                                                                   gaatgTTTTGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAgg                     <  1:360629/140‑1 (MQ=255)                                                                                                                          ttGATCAAACAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGaaaa              >  1:331208/1‑140 (MQ=255)                                                                                                                                 aaCAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGa       <  2:946856/140‑1 (MQ=255)                                                                                                                                  aCAGAGGGCAAAAAAAATAGCCATAAAAAAGTAAAAGTGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGag      >  1:1082263/1‑140 (MQ=255)                                                                                                                                    agagGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGagca    <  2:331208/140‑1 (MQ=255)                                                                                                                                      agGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGagcagc  <  2:55336/140‑1 (MQ=255)                                                                                                                                      agGGCAAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGagcagc  <  1:31469/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       CATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGC‑AAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAGAAAAGATGCGAGCAGC  >  NC_000913/1440797‑1441068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑