Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,479,687	(G)6→7	coding (550/1758 nt)	ynbC →	hydrolase/methyltransferase domain‑containing protein YnbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,479,681	1	.	G	100.0%	212.0 / NA	57	R182G (CGG→GGG)	ynbC	hydrolase/methyltransferase domain‑containing protein YnbC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (29/28);  total (29/28)

GGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTC  >  NC_000913/1479542‑1479810                                                                                                                                             |                                                                                                                                  ggTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAc                                                                                                                                    >  2:363625/1‑140 (MQ=255)       ggCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑gggggg                                                                                                                             <  1:749093/140‑1 (MQ=255)             tcCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGt                                                                                                                        >  1:832076/1‑140 (MQ=255)             tcCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGt                                                                                                                        >  1:611551/1‑140 (MQ=255)             tcCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGt                                                                                                                        >  2:294580/1‑140 (MQ=255)               cGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGttt                                                                                                                      >  2:331714/1‑140 (MQ=255)                  ccTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCaa                                                                                                                   >  2:1041970/1‑140 (MQ=255)                    tttAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCaata                                                                                                                 <  1:265974/140‑1 (MQ=255)                      tAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCaataat                                                                                                               >  2:1147272/1‑140 (MQ=255)                          gTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATc                                                                                                           <  1:294580/140‑1 (MQ=255)                              aaTTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCt                                                                                                       >  1:488327/1‑140 (MQ=255)                                     tgtgCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATcaca                                                                                                >  2:704604/1‑140 (MQ=255)                                          cGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGc                                                                                           >  2:358768/1‑140 (MQ=255)                                            cTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGa                                                                                         <  2:488327/140‑1 (MQ=255)                                            cTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGa                                                                                         <  1:961383/140‑1 (MQ=255)                                             tGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTTTTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGAt                                                                                        >  1:98386/1‑140 (MQ=255)                                               gCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTg                                                                                      <  1:358768/140‑1 (MQ=255)                                               gCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTg                                                                                      <  2:832076/140‑1 (MQ=255)                                                cACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGc                                                                                     <  1:1041970/140‑1 (MQ=255)                                                 aCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGcc                                                                                    >  1:941387/1‑140 (MQ=255)                                                         cgcTGGCGTTATGGCATCGTCTGCGTGGTGTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAatat                                                                            >  2:1168626/1‑140 (MQ=255)                                                           cTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGAtt                                                                                       >  1:333799/1‑127 (MQ=255)                                                           cTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGAtt                                                                                       <  2:333799/127‑1 (MQ=255)                                                              gCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGa                                                                                         <  2:126326/122‑1 (MQ=255)                                                              gCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGa                                                                                         >  1:126326/1‑122 (MQ=255)                                                                 ttATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCg                                                                    <  1:941997/140‑1 (MQ=255)                                                                   aTGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGAt                                                                  <  1:363625/140‑1 (MQ=255)                                                                   aTGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGAt                                                                  <  1:1182795/140‑1 (MQ=255)                                                                       cATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATctct                                                              >  1:817493/1‑140 (MQ=255)                                                                       cATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATctct                                                              <  2:494327/140‑1 (MQ=255)                                                                            tCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACaaa                                                         >  1:374438/1‑140 (MQ=255)                                                                              tGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAAc                                                       >  2:598290/1‑140 (MQ=255)                                                                                 gTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTc                                                    >  2:81369/1‑140 (MQ=255)                                                                                     tCTGTTTTTTTTTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGaa                                                <  2:98386/140‑1 (MQ=255)                                                                                     tCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGaa                                                <  2:611551/140‑1 (MQ=255)                                                                                     tCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGaa                                                >  2:360338/1‑140 (MQ=255)                                                                                       tGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACaaaa                                                        >  1:861570/1‑130 (MQ=255)                                                                                       tGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACaaaa                                                        <  2:861570/130‑1 (MQ=255)                                                                                       tGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                              <  2:402378/140‑1 (MQ=255)                                                                                       tGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCCGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                              <  1:360338/140‑1 (MQ=255)                                                                                          tttttATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtat                                           <  1:331714/140‑1 (MQ=255)                                                                                               attaATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                      >  2:256318/1‑140 (MQ=255)                                                                                                       cTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcg                              <  2:1095581/140‑1 (MQ=255)                                                                                                         aTGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggc                            <  2:374438/140‑1 (MQ=255)                                                                                                              aaaGGACGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGa                       >  2:895164/1‑140 (MQ=255)                                                                                                                   aCGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                  <  1:704604/140‑1 (MQ=255)                                                                                                                   aCGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                  <  1:1147272/140‑1 (MQ=255)                                                                                                                    cGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGc                 >  1:1168966/1‑140 (MQ=255)                                                                                                                    cGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGc                 >  2:443067/1‑140 (MQ=255)                                                                                                                    cGCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGc                 >  2:1165512/1‑140 (MQ=255)                                                                                                                     gCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCt                >  2:298295/1‑140 (MQ=255)                                                                                                                     gCTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCt                <  1:298295/140‑1 (MQ=255)                                                                                                                      cTATTTGACCCACGATCGGCAACGGGGGGGCGAGTTTAAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAATGTCTGAACGTATTGTTAGAGATGCGGCGGCGATTGCGCTc               >  2:1091805/1‑140 (MQ=255)                                                                                                                            gACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACg         <  1:443067/140‑1 (MQ=255)                                                                                                                            gACCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACg         <  1:1091805/140‑1 (MQ=255)                                                                                                                             aCCCACGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGc        <  2:1022247/140‑1 (MQ=255)                                                                                                                                 aCGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACt    >  2:226533/1‑140 (MQ=255)                                                                                                                                  cGATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACtt   <  2:216270/140‑1 (MQ=255)                                                                                                                                   gATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTc  >  2:1118109/1‑140 (MQ=255)                                                                                                                                   gATCGGCAACGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTc  >  1:434848/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                  GGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAAC‑GGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTC  >  NC_000913/1479542‑1479810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑