Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,997,140	(C)5→6	coding (675/753 nt)	tcyN ←	cystine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,997,135	1	.	C	100.0%	67.0 / NA	20	A227G (GCC→GGC)	tcyN	cystine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (8/12);  total (8/12)

TTTATCAGGCTTGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGG‑CCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGAC  >  NC_000913/1996999‑1997268                                                                                                                                          |                                                                                                                                      tttATCAGGCTTGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGG‑ccc                                                                                                                                    <  1:21333/140‑1 (MQ=255)           ttGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGa                                                                                                                           <  2:493404/140‑1 (MQ=255)           ttGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGa                                                                                                                           <  1:847292/140‑1 (MQ=255)                aTGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATc                                                                                                                      <  2:408977/140‑1 (MQ=255)                      tCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGcccc                                                                                                                <  1:673763/140‑1 (MQ=255)                       ccAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCt                                                                                                               <  1:137469/140‑1 (MQ=255)                                        aTCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGcc                                                                                              <  1:198595/140‑1 (MQ=255)                                              ggggCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGGCGGCAAAAAAAGCTTTTGCGGCCCCCCCCCTCGACTATCCGCCCCCGGGCCCTAAAAAACGCCCCGTcc                                                                                        >  2:922515/1‑140 (MQ=255)                                                          ttCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCgg                                                                            <  1:795257/140‑1 (MQ=255)                                                              tattatTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCaa                                                                        <  2:253884/140‑1 (MQ=255)                                                                       agcagAAACTTCTCGAGGAACTGGCGGGGGCGAGGCTGCTCGGGGGCGGCAAATAACGCTTTTGCGGCCCCCCCGCTCGACTATCCGCCCCTGGGCCATAAAAATCGCCCCGGCCGCCACATCCCCGGCAAAGCTCAttt                                                               >  2:225504/1‑140 (MQ=255)                                                                         cagAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAAGATCCCGGGCAAAGCTCATTTCg                                                             >  1:354529/1‑140 (MQ=255)                                                                           gAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGGCGGCAAATAAAGCTTTTTCGGCCCCCCCGCTCGACTATCCGCCCCCGGGCCATAAAAATCGCCCCGGCCGCAACATCCCCGGCAAAGCTCATTTCGTg                                                           >  2:673911/1‑140 (MQ=255)                                                                             aaCTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCg                                                         <  1:922515/140‑1 (MQ=255)                                                                             aaCTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCg                                                         <  1:1036630/140‑1 (MQ=255)                                                                                     gAGGAACTGGCGGGTGCGAGGCTTCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGGCCATAAAGATCGCCCCGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATc                                                 >  1:1130045/1‑140 (MQ=255)                                                                                                           tgctCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTgcg                           <  1:819523/140‑1 (MQ=255)                                                                                                             ctCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGcc                         >  2:117780/1‑140 (MQ=255)                                                                                                             ctCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGcc                         >  1:1048644/1‑140 (MQ=255)                                                                                                                       gCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCCCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGa               >  1:328878/1‑140 (MQ=255)                                                                                                                                   tttGCGGCCCCCCTGCTCGACTATCCGCCCCTGATCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGa   <  2:1068677/140‑1 (MQ=255)                                                                                                                                    ttGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGAc  >  2:1106578/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TTTATCAGGCTTGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGG‑CCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGAC  >  NC_000913/1996999‑1997268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑