Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,537,664 (T)6→7 coding (323/375 nt) yfeK → protein YfeK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,537,6581.T98.6% 248.7 / ‑5.9 71P106L (CCT→CTT) yfeKprotein YfeK
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base T (37/32);  minor base A (0/1);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTCTGAAGCTCGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑C‑TTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGA  >  NC_000913/2537521‑2537785
                                                                                                                                           |                                                                                                                               
gCGTCTGAAGCTCGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑C‑tt                                                                                                                               >  2:542618/1‑140 (MQ=255)
   tCTGAAGCTCGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑C‑ttttt                                                                                                                            >  1:1162951/1‑140 (MQ=255)
   tCTGAAGCTCGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑C‑ttttt                                                                                                                            >  2:429879/1‑140 (MQ=255)
      gAAGCTCGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑C‑ttttttta                                                                                                                         <  2:539374/140‑3 (MQ=255)
      gAAGCTCGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑C‑ttttttta                                                                                                                         <  1:619485/140‑3 (MQ=255)
      gAAGCTCGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑C‑ttttttta                                                                                                                         <  1:607056/140‑3 (MQ=255)
        aGCTCGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑C‑tttttttaca                                                                                                                       <  1:768894/140‑5 (MQ=255)
            cGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCg                                                                                                                    >  2:709055/1‑140 (MQ=255)
             ggCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGt                                                                                                                   >  2:280491/1‑140 (MQ=255)
                     ccGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTgcg                                                                                                           <  2:754980/140‑1 (MQ=255)
                      cGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTgcgc                                                                                                          <  2:924052/140‑1 (MQ=255)
                       gTAACCGCATTGACACTGCCGAGCAGTTTATTGGTAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCa                                                                                                         >  2:730802/1‑140 (MQ=255)
                          aCCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGAc                                                                                                      <  2:803561/140‑1 (MQ=255)
                          aCCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGAc                                                                                                      <  1:61868/140‑1 (MQ=255)
                            cGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACgg                                                                                                    >  2:1156218/1‑140 (MQ=255)
                              cATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGAt                                                                                                  >  2:685497/1‑140 (MQ=255)
                               aTTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATa                                                                                                 >  1:598493/1‑140 (MQ=255)
                               aTTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCATTAATTGCGCAGACGGATa                                                                                                 >  2:362103/1‑140 (MQ=255)
                                           gAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTg                                                                                     <  1:134726/140‑1 (MQ=255)
                                                   tATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCggaagga                                                                             >  2:903602/1‑140 (MQ=255)
                                                     ttGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGaaa                                                                           <  2:785699/140‑1 (MQ=255)
                                                        aTAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTa                                                                        >  1:524604/1‑140 (MQ=255)
                                                         tAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTaa                                                                       <  1:685497/140‑1 (MQ=255)
                                                          aaGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAAt                                                                      <  1:903602/140‑1 (MQ=255)
                                                          aaGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCATTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAAt                                                                      <  1:362103/140‑1 (MQ=255)
                                                           aGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGATGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATc                                                                     <  1:454668/140‑1 (MQ=255)
                                                           aGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATc                                                                     >  1:914764/1‑140 (MQ=255)
                                                           aGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATc                                                                     >  2:454668/1‑140 (MQ=255)
                                                           aGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATc                                                                     >  1:1093303/1‑140 (MQ=255)
                                                           aGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATc                                                                     >  1:741430/1‑140 (MQ=255)
                                                                gCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCtt                                                                >  1:371064/1‑140 (MQ=255)
                                                                gCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCt                                                                 <  2:335724/139‑1 (MQ=255)
                                                                gCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCt                                                                 >  1:335724/1‑139 (MQ=255)
                                                                  ttCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTg                                                              >  2:644438/1‑140 (MQ=255)
                                                                    cgtcgtCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTggg                                                            >  2:62182/1‑140 (MQ=255)
                                                                    cgtcgtCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTggg                                                            <  1:511602/140‑1 (MQ=255)
                                                                    cgtcgtCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTggg                                                            <  2:659145/140‑1 (MQ=255)
                                                                       cgtcATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAg                                                         <  1:1156218/140‑1 (MQ=255)
                                                                       cgtcATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAg                                                         <  1:454433/140‑1 (MQ=255)
                                                                       cgtcATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAg                                                         <  1:131253/140‑1 (MQ=255)
                                                                        gtcATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAgg                                                        <  2:598493/140‑1 (MQ=255)
                                                                        gtcATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAgg                                                        >  1:220232/1‑140 (MQ=255)
                                                                           aTCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCAt                                                     <  2:953133/140‑1 (MQ=255)
                                                                           aTCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCAt                                                     <  2:384536/140‑1 (MQ=255)
                                                                              gATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTa                                                  <  1:940015/140‑1 (MQ=255)
                                                                               aTTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTAc                                                 <  2:348457/140‑1 (MQ=255)
                                                                               aTTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTAc                                                 <  1:542618/140‑1 (MQ=255)
                                                                                ttACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACa                                                <  2:741430/140‑1 (MQ=255)
                                                                                ttACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACa                                                >  1:619952/1‑140 (MQ=255)
                                                                                      ggAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGt                                          >  2:485020/1‑140 (MQ=255)
                                                                                       gAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTa                                         <  1:410109/140‑1 (MQ=255)
                                                                                        aaGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTaa                                        >  2:216335/1‑140 (MQ=255)
                                                                                          gCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACa                                      >  2:930646/1‑140 (MQ=255)
                                                                                          gCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACa                                      >  2:492876/1‑140 (MQ=255)
                                                                                                   tgtgAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGaaa                                 <  2:773256/136‑1 (MQ=255)
                                                                                                   tgtgAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGaaa                                 >  1:773256/1‑136 (MQ=255)
                                                                                                    gtgAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACg                            <  1:485020/140‑1 (MQ=255)
                                                                                                     tgAAGATCCCCGGTAAGAGCGATGAGAACGCACAGCTT‑TTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGc                           >  1:982002/1‑140 (MQ=255)
                                                                                                     tgAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGt                                          >  2:953791/1‑125 (MQ=255)
                                                                                                     tgAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGt                                          <  1:953791/125‑1 (MQ=255)
                                                                                                     tgAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGc                           >  2:648166/1‑140 (MQ=255)
                                                                                                     tgAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGc                           >  1:787294/1‑140 (MQ=255)
                                                                                                      gAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCt                          <  2:220232/140‑1 (MQ=255)
                                                                                                      gAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCt                          <  1:266441/140‑1 (MQ=255)
                                                                                                          aTCCCCGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCtttat                      >  2:939835/1‑140 (MQ=255)
                                                                                                            ccccGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCtttattt                    >  2:521138/1‑140 (MQ=255)
                                                                                                            ccccGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCtttattt                    >  1:372510/1‑140 (MQ=255)
                                                                                                             cccGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGaaaa                                <  2:297900/127‑1 (MQ=255)
                                                                                                             cccGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGaaaa                                >  1:297900/1‑127 (MQ=255)
                                                                                                             cccGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCtttattta                   >  2:959988/1‑140 (MQ=255)
                                                                                                             cccGGTAAGAGCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCtttattta                   >  1:665142/1‑140 (MQ=255)
                                                                                                                ggTAAGAGCGATGAGAACGCACAGC‑CATTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTCCAGGAGGTAACATGAAAAAACGCTTTATTTATCa                <  2:1093303/140‑1 (MQ=255)
                                                                                                                    agagCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGAt            >  1:934196/1‑140 (MQ=255)
                                                                                                                      agCGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGa          >  1:472266/1‑140 (MQ=255)
                                                                                                                        cGATGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGaaa        >  2:157739/1‑140 (MQ=255)
                                                                                                                          aTGAGAACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGaaaaa      >  2:681885/1‑140 (MQ=255)
                                                                                                                              gaACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGa  <  1:939835/140‑1 (MQ=255)
                                                                                                                              gaACGCACAGC‑CTTTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGa  <  2:619952/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                               
GCGTCTGAAGCTCGGCAATACCCGTAACCGCATTGACACTGCCGAGCAGTTTATTGATAAGGTTGCTTCGTCGTCATCGATTACTGGGAAGCCGTATATTGTGAAGATCCCCGGTAAGAGCGATGAGAACGCACAGC‑C‑TTTTTTACATGCGTTAATTGCGCAGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGA  >  NC_000913/2537521‑2537785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑