Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,326,4500TG21.3% 110.0 / 11.6 47G114G (GGT→GGG) atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (2/35);  new base G (10/0);  total (12/35)
Fisher's exact test for biased strand distribution p-value = 1.27e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGAT  >  NC_000913/2326314‑2326587
                                                                                                                                        |                                                                                                                                          
cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATg                                                                                                                                         <  1:992641/140‑1 (MQ=255)
   tGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaa                                                                                                                                      <  1:356385/140‑1 (MQ=255)
        aaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAatat                                                                                                                                 <  1:1139996/140‑1 (MQ=255)
        aaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAatat                                                                                                                                 <  1:13287/140‑1 (MQ=255)
             cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTa                                                                                                                                           <  1:343033/125‑1 (MQ=255)
               ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTa                                                                                                                          <  1:1071864/140‑1 (MQ=255)
               ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTa                                                                                                                          >  2:169460/1‑140 (MQ=255)
               ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGCGtttt                                                                                                                          >  2:768516/1‑139 (MQ=255)
                 cTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGc                                                                                                                        <  1:1013908/140‑1 (MQ=255)
                  tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGcc                                                                                                                       <  1:169460/140‑1 (MQ=255)
                  tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGcc                                                                                                                       <  2:183135/140‑1 (MQ=255)
                  tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGcc                                                                                                                       <  1:1074563/140‑1 (MQ=255)
                   ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccc                                                                                                                      <  2:529243/140‑1 (MQ=255)
                    gCAGAAATGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGcccc                                                                                                                     <  2:90521/140‑1 (MQ=255)
                              tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGttt                                                                                                                           <  2:700277/124‑1 (MQ=255)
                              tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTa                                                                                                                          <  2:862709/125‑1 (MQ=255)
                              tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTc                                                                                                           <  1:345129/140‑1 (MQ=255)
                              tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGAGTTTTGCCCCCTTCTTACTc                                                                                                           >  2:908805/1‑140 (MQ=255)
                              tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGttt                                                                                                                           >  1:700277/1‑124 (MQ=255)
                              tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTa                                                                                                                          >  1:862709/1‑125 (MQ=255)
                                        cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAg                                                                                                 <  1:470932/140‑1 (MQ=255)
                                          cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCa                                                                                               <  2:781040/140‑1 (MQ=255)
                                           cagcAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAc                                                                                              <  1:637168/137‑1 (MQ=255)
                                              cAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGct                                                                                           <  2:533332/140‑1 (MQ=255)
                                                     gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                                                                                    <  2:1098456/140‑1 (MQ=255)
                                                     gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                                                                                    <  1:710094/140‑1 (MQ=255)
                                                         gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                                                                                    <  2:75709/136‑1 (MQ=255)
                                                         gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGAAAAAATGAGTTTTACCCCCCACTTACTCGATGCAAAAACACGCCCTGGttttttt                                                                                >  2:637168/1‑135 (MQ=255)
                                                         gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                                                                                    >  1:75709/1‑136 (MQ=255)
                                                         gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGGTTAGCCCCCCACTTACTCGATGCAAAAACACGCCCTGGTTATCgg                                                                                >  2:465634/1‑139 (MQ=255)
                                                             ttCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTTCTCGATGCAAAAAAACGCCCTGGTTTTCGTCttt                                                                            >  2:438057/1‑139 (MQ=255)
                                                               cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGTGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGga                                                                          >  2:356046/1‑140 (MQ=255)
                                                                gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCCCTGGTTATCGTCTTTgag                                                                         >  2:883005/1‑140 (MQ=255)
                                                                gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCCCTGGGTATCGTCTTGgag                                                                         >  1:1098426/1‑140 (MQ=255)
                                                                     ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa                                                                    <  1:933208/140‑1 (MQ=255)
                                                                      agaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc                                                                   <  1:134349/138‑1 (MQ=255)
                                                                            tgtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGttt                                                            >  2:1127902/1‑140 (MQ=255)
                                                                             gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTa                                                           <  1:566292/140‑1 (MQ=255)
                                                                             gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGgttt                                                           >  1:635639/1‑139 (MQ=255)
                                                                              tgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCCACTTTCTCGATGCAAAAACACGCTCTGGTTATCGTCTTGGAGACGGGC‑AGGGTTAt                                                          >  2:566292/1‑140 (MQ=255)
                                                                               ggCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATTAGTTTTGCCCCCCCCTTTCTCCATGCAAAAAAACGCCCTTGTTATCGTCTTGGGGACGGACAAGGttttt                                                          >  2:933208/1‑138 (MQ=255)
                                                                                  gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACg                                                      <  1:438057/140‑1 (MQ=255)
                                                                                   cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCTCTGGGTATCGTCTTGGGGACGGGC‑AGGGTTATTAcct                                                     >  2:134349/1‑138 (MQ=255)
                                                                                              ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGTGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGcgcg                                          <  1:356046/140‑1 (MQ=255)
                                                                                              ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGGTATCGTCTTTGAGACGGAC‑AGGGTTATGACGTAATCCTGcgcg                                          >  2:856137/1‑140 (MQ=255)
                                                                                                        gcaggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGAAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGAt                                <  1:910819/140‑1 (MQ=255)
                                                                                                                 gcgcAGAGCATTGTGGCGGGGGGGGTGGGAAAAAAAAGGTTTGGCCCCCCCCTACTCCATGCAAAAAAACCCCCTTGTTTTTGTCTTTGGGACGGGC‑AGGGTTTTTAAGTAAACCTGCGCGGTGGGCCGCTGTGCGcccc                       >  2:604870/1‑138 (MQ=255)
                                                                                                                 gcgcAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCCCTGGTTATTGTCTTTGGGACGGAC‑AGGGTTATTACGTAATCCTGCGCGGTGGCCCGGTGTGCGcccc                       >  2:188395/1‑138 (MQ=255)
                                                                                                                    gagacagttGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCa                    <  1:980881/133‑1 (MQ=255)
                                                                                                                    cAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCa                    <  2:532428/140‑1 (MQ=255)
                                                                                                                       agCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATgg                 <  2:626248/140‑1 (MQ=255)
                                                                                                                       agCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATgg                 <  1:883005/140‑1 (MQ=255)
                                                                                                                          aTTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTa              <  1:908805/140‑1 (MQ=255)
                                                                                                                           ttGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTTCTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGGTTATTACGTAATCCTGCGCGATGGGCTGATGTGCGCCACCCATGGTTAt             >  2:135586/1‑140 (MQ=255)
                                                                                                                                 cGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATg       <  1:768516/140‑1 (MQ=255)
                                                                                                                                      ggTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGaa  <  1:856137/140‑2 (MQ=255)
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CACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC‑AGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGAT  >  NC_000913/2326314‑2326587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑