Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,580,0950AC22.9% 103.7 / 12.5 48G456G (GGT→GGG) hsdStype I restriction enzyme EcoKI specificity protein
Reads supporting (aligned to +/- strand):  ref base A (30/7);  new base C (0/11);  total (30/18)
Fisher's exact test for biased strand distribution p-value = 1.41e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAATAGAATAAAATGGGCAATTTCTGGAATGATTTAAATATATTTATGTGGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTGCCAGGATGGA  >  NC_000913/4579958‑4580229
                                                                                                                                         |                                                                                                                                      
aaaTAGAATAAAATGGGCAATTTCTGGAATGATTTAAATATATTTATGTGGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTAcc                                                                                                                                      <  1:86331/140‑1 (MQ=255)
           aaTGGGCAATTTCTGGAATGATTTAAATATATTTATGTGGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTg                                                                                                                           >  1:841682/1‑140 (MQ=255)
                gCAATTTCTGGAATGATTTAAATATATTTATGTGGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGt                                                                                                                      >  2:634092/1‑140 (MQ=255)
                    tttCTGGAATGATTTAAATATATTTATGTGGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAg                                                                                                                  >  2:559089/1‑140 (MQ=255)
                    tttCTGGAATGATTTAAATATATTTATGTGGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAg                                                                                                                  >  2:589342/1‑140 (MQ=255)
                               aTTTAAATATATTTATGTGGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGAttttt                                                                                                       >  1:758459/1‑140 (MQ=255)
                                          tttATGTGGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAAcgc                                                                                            >  1:705980/1‑140 (MQ=255)
                                              tgtgGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACgcggcg                                                                                        >  2:1025080/1‑140 (MQ=255)
                                                      aTGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGgcgc                                                                                    >  1:1129110/1‑136 (MQ=255)
                                                      aTGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGgcgc                                                                                    <  2:1129110/136‑1 (MQ=255)
                                                            ggCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTcc                                                                          >  1:1009370/1‑140 (MQ=255)
                                                             gCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCg                                                                         >  1:454430/1‑140 (MQ=255)
                                                             gCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCg                                                                         >  2:1235192/1‑140 (MQ=255)
                                                             gCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCg                                                                         >  2:623796/1‑140 (MQ=255)
                                                              cGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGc                                                                        >  2:1227751/1‑140 (MQ=255)
                                                                               cgcACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGttt                                                       >  2:163018/1‑140 (MQ=255)
                                                                               cgcACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGC‑TTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGtttt                                                      <  2:595313/140‑1 (MQ=255)
                                                                                  cccGAAAAGGTGCGCAAAAAAATAATGTTCAGGATTTTTTACGTGAGGTTTTTTTCCCCCCGCTAGCGGCGCGTTCAGTTTTGTTTTTTCCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGTTTTTCg                                                    <  2:1009370/139‑1 (MQ=255)
                                                                                     ggAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGcc                                                 <  2:705980/140‑1 (MQ=255)
                                                                                          ggTGCGCAAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCa                                            <  1:457900/140‑1 (MQ=255)
                                                                                            tGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACt                                          >  2:503180/1‑140 (MQ=255)
                                                                                            tGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACt                                          >  1:418125/1‑140 (MQ=255)
                                                                                              cgcCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTgg                                        >  2:137504/1‑140 (MQ=255)
                                                                                              cgcCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTgg                                        >  2:69388/1‑140 (MQ=255)
                                                                                                ccAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACt                                          <  1:198523/136‑1 (MQ=255)
                                                                                                ccAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACt                                          >  2:198523/1‑136 (MQ=255)
                                                                                                 cAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGTTTTTCGGCCCGCCACTGGGCg                                     <  1:1227751/140‑1 (MQ=255)
                                                                                                 cAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCg                                     <  1:137504/140‑1 (MQ=255)
                                                                                                 cAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCg                                     >  1:247869/1‑140 (MQ=255)
                                                                                                   aaaaaTAATGTTCAGGATTTTTTACGTGAGGCTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGt                                   <  1:69388/139‑1 (MQ=255)
                                                                                                     aaaTAATGTTCAGGATTTTTTACGTGAGGCTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTgg                                        <  1:865136/133‑1 (MQ=255)
                                                                                                     aaaTAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTgg                                        >  2:865136/1‑133 (MQ=255)
                                                                                                        aaatGTTCAGGATTTTTTACGTGAGGTTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGTTTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGtt                              <  2:370964/139‑1 (MQ=255)
                                                                                                           tGTTCAGGATTTTTTACGTGAGGCTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATAAAACCCGGGTTTCCGGCCCGCCACTGGGCGGTAAGTTcac                           <  1:1235192/140‑1 (MQ=255)
                                                                                                             ttCAGGATTTTTTACGTGAGGCTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTTTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGt                                   <  2:319537/130‑1 (MQ=255)
                                                                                                             ttCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGt                                   >  1:319537/1‑130 (MQ=255)
                                                                                                                aGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACgg                      >  2:625462/1‑140 (MQ=255)
                                                                                                                  gATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGaa                    >  1:290363/1‑140 (MQ=255)
                                                                                                                       tttACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCtt               >  2:868908/1‑140 (MQ=255)
                                                                                                                          aCGTGAGGTTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGTTTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGTTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTg            <  2:591925/140‑1 (MQ=255)
                                                                                                                            gTGAGGCTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTGcc          <  1:1025080/140‑1 (MQ=255)
                                                                                                                               aGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTGCCAgg       >  1:822915/1‑140 (MQ=255)
                                                                                                                                ggCTTTTTTCCCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGc                 <  1:1220928/129‑1 (MQ=255)
                                                                                                                                ggCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGc                 >  2:1220928/1‑129 (MQ=255)
                                                                                                                                 gCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTGCCAggat     >  1:941177/1‑140 (MQ=255)
                                                                                                                                 gCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTGCCAggat     >  1:1148302/1‑140 (MQ=255)
                                                                                                                                   ttttttACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTGCCAggatgg   <  1:868908/140‑1 (MQ=255)
                                                                                                                                    tttttCCCCCCGTTAGCGGCGCTTTCAGTTTTGATTTTTCCCAGCAGCGGGGCGGCGCTGTTTTCCCCGTTGATAAAATCGGGTTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTGCCAggatgga  <  2:180105/140‑1 (MQ=255)
                                                                                                                                    tttttCCCCCCGCTAGCTGCGCGTTCAGCTTTGTTTTTTCCCAGCACCGCGGCGGCGCTGTTTTCCCCGCTGATCAAACCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTGCCAggatgga  <  2:290363/140‑1 (MQ=255)
                                                                                                                                     ttttACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTg            >  2:1173006/1‑129 (MQ=255)
                                                                                                                                     ttttACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTg            <  1:1173006/129‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
AAATAGAATAAAATGGGCAATTTCTGGAATGATTTAAATATATTTATGTGGGTTATGATTGGCGTGAAATAATAAAAAGCGCACCGGAAAGGTGCGCCAGAAAATAATGTTCAGGATTTTTTACGTGAGGCTTTTTTACCCCCGCTAGCTGCGCGTTCAGCTTTGATTTTTTCCAGCAACGCGGCGGCGCTGTTTTCTCCGCTGATCAAATCCGGGTTTTCGGCCCGCCACTGGGCGGTAAGTTCACCACGGAACGCTTTTGCCAGGATGGA  >  NC_000913/4579958‑4580229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑