Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	95,385	A→G	S246G (AGC→GGC)	murF →	D‑alanyl‑D‑alanine‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	95,385	0	A	G	100.0%	178.2 / NA	49	S246G (AGC→GGC)	murF	D‑alanyl‑D‑alanine‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/23);  total (26/23)

AGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTG  >  NC_000913/95249‑95520                                                                                                                                         |                                                                                                                                        agcgaaagGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCg                                                                                                                                      >  1:434327/1‑140 (MQ=255) agcgaaagGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCg                                                                                                                                      >  1:672408/1‑140 (MQ=255)        ggTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCAc                                                                                                                               <  2:1160413/140‑1 (MQ=255)          tGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGACCTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCg                                                                                                                             <  1:888631/140‑1 (MQ=255)          tGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCg                                                                                                                             <  1:432007/140‑1 (MQ=255)          tGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGAGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCg                                                                                                                             >  2:1006530/1‑140 (MQ=255)           gAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGc                                                                                                                            <  2:211291/140‑1 (MQ=255)           gAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGc                                                                                                                            <  2:243967/140‑1 (MQ=255)                   tAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCTAACGGCGATTTCACCGCCACCAata                                                                                                                    >  1:675457/1‑140 (MQ=255)                   tAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAata                                                                                                                    <  1:517624/140‑1 (MQ=255)                    aGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAACGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAatat                                                                                                                   >  2:1120681/1‑140 (MQ=255)                         ccTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCAtg                                                                                                              <  1:884737/140‑1 (MQ=255)                           tGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCAtgtg                                                                                                            >  2:817487/1‑140 (MQ=255)                                   aaCGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCa                                                                                                    <  2:458434/140‑1 (MQ=255)                                   aaCGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCa                                                                                                    <  1:817487/140‑1 (MQ=255)                                   aaCGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCa                                                                                                    <  2:233374/140‑1 (MQ=255)                                   aaCGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGAGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCa                                                                                                    <  1:1006530/140‑1 (MQ=255)                                                 tATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTa                                                                                      <  2:723329/140‑1 (MQ=255)                                                 tATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTa                                                                                      <  1:1120681/140‑1 (MQ=255)                                                  aTGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTAc                                                                                     >  2:745061/1‑140 (MQ=255)                                                   tGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTAcc                                                                                    <  2:645818/140‑1 (MQ=255)                                                        gCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACa                                                                               >  2:526090/1‑140 (MQ=255)                                                        gCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACa                                                                               >  1:833839/1‑140 (MQ=255)                                                           gACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAAc                                                                            >  1:1019969/1‑140 (MQ=255)                                                                          cTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGt                                                             >  1:941271/1‑140 (MQ=255)                                                                           tGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTc                                                            >  1:876940/1‑140 (MQ=255)                                                                                   cAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTct                                                    >  1:951022/1‑140 (MQ=255)                                                                                   cAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTct                                                    >  2:274752/1‑140 (MQ=255)                                                                                          tAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCg                                             <  1:526090/140‑1 (MQ=255)                                                                                                 cTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCTAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgg                                      <  2:675457/140‑1 (MQ=255)                                                                                                   cACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgggg                                    <  2:876940/140‑1 (MQ=255)                                                                                                     cGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgggg                                    <  2:17438/138‑1 (MQ=255)                                                                                                     cGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgggg                                    >  1:17438/1‑138 (MQ=255)                                                                                                     cGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCg                                  <  2:951022/140‑1 (MQ=255)                                                                                                     cGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCg                                  >  1:639254/1‑140 (MQ=255)                                                                                                     cGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCg                                  >  2:622457/1‑140 (MQ=255)                                                                                                              tgGCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAatat                         >  1:1068573/1‑140 (MQ=255)                                                                                                                gCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgggg                                    <  2:1205215/127‑1 (MQ=255)                                                                                                                gCGTTTCTCACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgggg                                    >  1:1205215/1‑127 (MQ=255)                                                                                                                      ctcACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATg                 >  2:597312/1‑140 (MQ=255)                                                                                                                      ctcACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATg                 >  2:566981/1‑140 (MQ=255)                                                                                                                      ctcACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATg                 >  2:541369/1‑140 (MQ=255)                                                                                                                      ctcACCCAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATg                 >  2:250794/1‑140 (MQ=255)                                                                                                                           ccAATGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTg            >  2:413647/1‑140 (MQ=255)                                                                                                                             aaTGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGc          >  2:552809/1‑140 (MQ=255)                                                                                                                             aaTGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGc          <  2:941271/140‑1 (MQ=255)                                                                                                                              aTGCCGCCAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCa         >  2:798222/1‑140 (MQ=255)                                                                                                                                  cgccAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCg     <  2:1068573/140‑1 (MQ=255)                                                                                                                                     cAACGGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTg  <  1:566981/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTG  >  NC_000913/95249‑95520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑