Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,173,650	T→C	T583A (ACT→GCT)	parE ←	DNA topoisomerase IV subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,173,650	0	T	C	100.0%	264.7 / NA	72	T583A (ACT→GCT)	parE	DNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/32);  total (40/32)

ATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCG  >  NC_000913/3173514‑3173786                                                                                                                                         |                                                                                                                                         aTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGtt                                                                                                                                       <  1:543250/140‑1 (MQ=255) aTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGtt                                                                                                                                       <  1:366736/140‑1 (MQ=255)  tctcCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTc                                                                                                                                      >  2:224493/1‑140 (MQ=255)  tctcCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTc                                                                                                                                      >  2:969535/1‑140 (MQ=255)      cGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGAt                                                                                                                                  >  1:1027617/1‑140 (MQ=255)           tGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGc                                                                                                                             >  1:887248/1‑140 (MQ=255)            gTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCg                                                                                                                            >  2:103689/1‑140 (MQ=255)            gTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCg                                                                                                                            <  1:514555/140‑1 (MQ=255)                    tctcTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTcg                                                                                                                    <  2:1027617/140‑1 (MQ=255)                              ccAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCa                                                                                                          <  1:186179/140‑1 (MQ=255)                               cAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCAt                                                                                                         <  1:1198054/140‑1 (MQ=255)                               cAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCAt                                                                                                         <  1:432902/140‑1 (MQ=255)                                         gATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCAtt                                                                                               <  2:1060460/140‑1 (MQ=255)                                          aTCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCAttt                                                                                              >  2:834780/1‑140 (MQ=255)                                          aTCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCAttt                                                                                              >  1:1039193/1‑140 (MQ=255)                                              tCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTcccc                                                                                          <  2:887248/140‑1 (MQ=255)                                                cGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCa                                                                                        <  1:541188/140‑1 (MQ=255)                                                cGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCAGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCa                                                                                        <  2:757282/140‑1 (MQ=255)                                                   gCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGAc                                                                                     <  1:641101/140‑1 (MQ=255)                                                       ttcttcGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCttt                                                                                 >  1:52379/1‑140 (MQ=255)                                                             gCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACg                                                                           <  1:97748/140‑1 (MQ=255)                                                               cagcagCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCt                                                                         <  1:103689/140‑1 (MQ=255)                                                                agcagcATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTg                                                                        <  2:52379/140‑1 (MQ=255)                                                                  cagcaTATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGa                                                                      >  1:712811/1‑140 (MQ=255)                                                                  cagcaTATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGa                                                                      >  2:690390/1‑140 (MQ=255)                                                                     caTATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGTACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGt                                                                   <  2:477489/140‑1 (MQ=255)                                                                     caTATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGt                                                                   >  2:391540/1‑140 (MQ=255)                                                                     caTATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGt                                                                   >  2:698625/1‑140 (MQ=255)                                                                      atatCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGtt                                                                  <  2:660069/140‑1 (MQ=255)                                                                       tatCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTc                                                                 >  1:221163/1‑140 (MQ=255)                                                                           catcatCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCt                                                             <  1:698625/140‑1 (MQ=255)                                                                           catcatCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCt                                                             <  1:391540/140‑1 (MQ=255)                                                                             tcatcGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTg                                                           >  2:918113/1‑140 (MQ=255)                                                                              catcGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGc                                                          <  1:969535/140‑1 (MQ=255)                                                                              catcGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGc                                                          <  1:224493/140‑1 (MQ=255)                                                                               atcGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACg                                                                    >  2:499173/1‑129 (MQ=255)                                                                               atcGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACg                                                                    <  1:499173/129‑1 (MQ=255)                                                                               atcGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGcc                                                         >  1:832163/1‑140 (MQ=255)                                                                                tcGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCt                                                        <  1:690390/140‑1 (MQ=255)                                                                                   cgTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTc                                                     >  2:45306/1‑140 (MQ=255)                                                                                   cgTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTc                                                     >  1:1073524/1‑140 (MQ=255)                                                                                       aGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGc                                                 >  1:208063/1‑140 (MQ=255)                                                                                         tACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGt                                               <  2:208063/140‑1 (MQ=255)                                                                                                aTCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAAtt                                        >  2:610555/1‑140 (MQ=255)                                                                                                aTCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAAtt                                        >  1:1234103/1‑140 (MQ=255)                                                                                                aTCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAAtt                                        >  2:489726/1‑140 (MQ=255)                                                                                                aTCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAAtt                                        >  2:57880/1‑140 (MQ=255)                                                                                                   gTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCt                                     >  1:415698/1‑140 (MQ=255)                                                                                                   gTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCt                                     >  2:120584/1‑140 (MQ=255)                                                                                                   gTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCt                                     >  2:45731/1‑140 (MQ=255)                                                                                                          tcatcGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTAc                              >  2:1176153/1‑140 (MQ=255)                                                                                                           catcGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACg                             <  2:352959/140‑1 (MQ=255)                                                                                                             ccGATAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGcc                           <  1:918113/139‑1 (MQ=255)                                                                                                                aTAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCctc                        >  1:1099024/1‑140 (MQ=255)                                                                                                                 tAGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCctct                       <  1:1176153/140‑1 (MQ=255)                                                                                                                  aGTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCtt                      >  1:538880/1‑140 (MQ=255)                                                                                                                   gTCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCttt                     >  1:689409/1‑140 (MQ=255)                                                                                                                    tCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGcc                           >  1:694494/1‑133 (MQ=255)                                                                                                                    tCAACTGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGcc                           <  2:694494/133‑1 (MQ=255)                                                                                                                         tGCACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCtcttc               >  2:381446/1‑140 (MQ=255)                                                                                                                           cACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTcttctt             >  1:991518/1‑140 (MQ=255)                                                                                                                           cACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTcttctt             >  1:672235/1‑140 (MQ=255)                                                                                                                           cACCAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTcttctt             >  1:349794/1‑140 (MQ=255)                                                                                                                             ccAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTtct                   >  1:687765/1‑132 (MQ=255)                                                                                                                             ccAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTtct                   <  2:687765/132‑1 (MQ=255)                                                                                                                             ccAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTcc           >  1:142696/1‑140 (MQ=255)                                                                                                                              cAGACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCg          >  2:994270/1‑140 (MQ=255)                                                                                                                                gACGGCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTc        >  1:898630/1‑140 (MQ=255)                                                                                                                                   ggCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAgc     >  2:172186/1‑140 (MQ=255)                                                                                                                                    gCGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAgcg    <  2:1057820/140‑1 (MQ=255)                                                                                                                                     cGAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAgcgc   <  1:994270/140‑1 (MQ=255)                                                                                                                                      gAGCGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGcgcg  <  1:489726/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGTTTCGCGCAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCG  >  NC_000913/3173514‑3173786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑