Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	423,359	A→G	Y281C (TAC→TGC)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	423,359	0	A	G	100.0%	254.5 / NA	70	Y281C (TAC→TGC)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/30);  total (40/30)

GCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCG  >  NC_000913/423228‑423495                                                                                                                                    |                                                                                                                                         gcagcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTa                                                                                                                                  <  1:103046/140‑1 (MQ=255)  cagcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAg                                                                                                                                 >  2:580839/1‑140 (MQ=255)     cTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGtga                                                                                                                              >  2:475275/1‑140 (MQ=255)     cTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGtga                                                                                                                              >  2:1063396/1‑140 (MQ=255)     cTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGtga                                                                                                                              >  2:718633/1‑140 (MQ=255)     cTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGtga                                                                                                                              >  1:17066/1‑140 (MQ=255)             gCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCa                                                                                                                      <  1:665792/140‑1 (MQ=255)                      gCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCg                                                                                                             <  1:718633/140‑1 (MQ=255)                       cTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGa                                                                                                            <  2:971164/140‑1 (MQ=255)                       cTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGa                                                                                                            <  1:569669/140‑1 (MQ=255)                       cTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGa                                                                                                            <  2:848531/140‑1 (MQ=255)                              gCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACtggctt                                                                                                     >  1:697878/1‑140 (MQ=255)                               cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                                                                                    >  1:866901/1‑140 (MQ=255)                                         cacaGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAg                                                                                          <  2:126074/140‑1 (MQ=255)                                           caGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCt                                                                                        >  2:868636/1‑140 (MQ=255)                                             gTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTg                                                                                      >  1:946028/1‑140 (MQ=255)                                             gTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTg                                                                                      >  2:1173326/1‑140 (MQ=255)                                             gTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTg                                                                                      >  2:872848/1‑140 (MQ=255)                                             gTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTg                                                                                      >  2:240651/1‑140 (MQ=255)                                              tGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGc                                                                                     >  1:197160/1‑140 (MQ=255)                                                 cGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGa                                                                                  >  2:684359/1‑140 (MQ=255)                                                  gATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGaa                                                                                 <  1:868636/140‑1 (MQ=255)                                                   aTTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAg                                                                                <  2:197160/140‑1 (MQ=255)                                                   aTTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAg                                                                                <  1:514258/140‑1 (MQ=255)                                                    ttCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGc                                                                               >  2:482392/1‑140 (MQ=255)                                                     tCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCt                                                                              >  2:375295/1‑140 (MQ=255)                                                        cATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTTGACTGGCTTGGCTATGCCAGCTTGCCGAAGc                                                                               <  2:314048/136‑1 (MQ=255)                                                        cATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTTGACTGGCTTGGCTATGCCAGCTTGCCGAAGc                                                                               >  1:314048/1‑136 (MQ=255)                                                         aTGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGAt                                                                          <  2:697878/140‑1 (MQ=255)                                                               ggTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAg                                                                    >  1:537472/1‑140 (MQ=255)                                                                gTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGt                                                                   >  1:853920/1‑140 (MQ=255)                                                                 tttGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTc                                                                  >  1:921562/1‑140 (MQ=255)                                                                    gACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGa                                                               <  1:240651/140‑1 (MQ=255)                                                                      cAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGaaa                                                             >  2:437964/1‑140 (MQ=255)                                                                           aTAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTg                                                        <  2:921562/140‑1 (MQ=255)                                                                               tCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGtga                                                    <  1:437964/140‑1 (MQ=255)                                                                               tCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGtga                                                    >  1:66530/1‑140 (MQ=255)                                                                               tCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGt                                                      <  2:85124/138‑1 (MQ=255)                                                                               tCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGt                                                      >  1:85124/1‑138 (MQ=255)                                                                                cGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGtgaa                                                   <  1:684359/140‑1 (MQ=255)                                                                                   ggCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGtgaatga                                                >  1:92213/1‑140 (MQ=255)                                                                                          gtggtgCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTAt                                         >  2:721678/1‑140 (MQ=255)                                                                                               gCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGGCTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCgg                                    >  2:1121849/1‑140 (MQ=255)                                                                                               gCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCgg                                    <  1:1173326/140‑1 (MQ=255)                                                                                                cTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCggg                                   <  1:324507/140‑1 (MQ=255)                                                                                                  tGTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGTGGa                                 <  2:17066/140‑1 (MQ=255)                                                                                                   gTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGaa                                >  1:1148218/1‑140 (MQ=255)                                                                                                   gTCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGaa                                >  2:1068495/1‑140 (MQ=255)                                                                                                    tCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGa                                 >  1:186713/1‑138 (MQ=255)                                                                                                    tCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGa                                 <  2:186713/138‑1 (MQ=255)                                                                                                    tCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAg                               >  1:209118/1‑140 (MQ=255)                                                                                                    tCACAACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAg                               >  2:503347/1‑140 (MQ=255)                                                                                                      acaACCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGAc                             <  1:1197412/140‑1 (MQ=255)                                                                                                        aaCCCCGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAg                           >  1:698024/1‑140 (MQ=255)                                                                                                          ccccGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTa                         <  2:960650/140‑1 (MQ=255)                                                                                                             cGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTa                         >  2:976498/1‑137 (MQ=255)                                                                                                             cGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTa                         <  2:709370/137‑1 (MQ=255)                                                                                                             cGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTa                         <  1:976498/137‑1 (MQ=255)                                                                                                             cGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTa                         >  1:709370/1‑137 (MQ=255)                                                                                                             cGAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTg                      >  1:240787/1‑140 (MQ=255)                                                                                                              gAATCGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGt                     >  1:194265/1‑140 (MQ=255)                                                                                                                  cGCCCTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGc                 <  1:872848/140‑1 (MQ=255)                                                                                                                    cccTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGc                 <  2:375988/138‑1 (MQ=255)                                                                                                                    cccTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGc                 >  1:375988/1‑138 (MQ=255)                                                                                                                    cccTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCa               >  1:363184/1‑140 (MQ=255)                                                                                                                     ccTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCAc              <  1:482392/140‑1 (MQ=255)                                                                                                                      cTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACt             >  2:911255/1‑140 (MQ=255)                                                                                                                      cTGGCGCGACTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACt             <  2:946028/140‑1 (MQ=255)                                                                                                                              aCTGGTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGaaa     >  2:220970/1‑140 (MQ=255)                                                                                                                                 ggTGCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAACGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAgcg  <  2:853920/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         GCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCG  >  NC_000913/423228‑423495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑