Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,683,627	T→C	intergenic (‑180/+3)	dctA ← / ← pdeK	C4 dicarboxylate/orotate:H(+) symporter/putative c‑di‑GMP phosphodiesterase PdeK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,683,627	0	T	C	100.0%	219.0 / NA	59	intergenic (‑180/+3)	dctA/pdeK	C4 dicarboxylate/orotate:H(+) symporter/putative c‑di‑GMP phosphodiesterase PdeK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/34);  total (25/34)

TACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCAGTCTCCACGCCTTCGGCAA  >  NC_000913/3683492‑3683763                                                                                                                                        |                                                                                                                                         tACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCt                                                                                                                                      <  2:193381/140‑1 (MQ=255) tACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCt                                                                                                                                      <  2:1156685/140‑1 (MQ=255)  aCCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTa                                                                                                                                     >  2:467022/1‑140 (MQ=255)  aCCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTa                                                                                                                                     >  1:705803/1‑140 (MQ=255)  aCCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTa                                                                                                                                     >  1:632571/1‑140 (MQ=255)   ccTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTAc                                                                                                                                    >  1:316636/1‑140 (MQ=255)     tGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACtt                                                                                                                                  <  2:203618/140‑1 (MQ=255)     tGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACtt                                                                                                                                  <  1:467022/140‑1 (MQ=255)      gCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGTATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACttt                                                                                                                                 >  2:1151755/1‑140 (MQ=255)      gCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACttt                                                                                                                                 >  2:973070/1‑140 (MQ=255)      gCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACttt                                                                                                                                 <  2:511481/140‑1 (MQ=255)       cgtccTTAAAATAACACCCAGCCAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACtttt                                                                                                                                >  2:983152/4‑140 (MQ=255)            ttAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTcc                                                                                                                           <  2:307062/140‑1 (MQ=255)             tAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCt                                                                                                                                      <  2:859398/128‑1 (MQ=255)             tAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCt                                                                                                                                      >  1:859398/1‑128 (MQ=255)             tAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCa                                                                                                                          >  1:269333/1‑140 (MQ=255)                  tAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTaa                                                                                                                     <  2:1069574/140‑1 (MQ=255)                    acacCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAAct                                                                                                                   <  2:466222/140‑1 (MQ=255)                          aGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTc                                                                                                             >  2:64973/1‑140 (MQ=255)                           gACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCg                                                                                                            >  2:851120/1‑140 (MQ=255)                           gACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCg                                                                                                            <  1:1151755/140‑1 (MQ=255)                             caacaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                                                          <  2:953246/140‑1 (MQ=255)                             caacaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                                                          >  2:590784/1‑140 (MQ=255)                               acaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                        <  2:316636/140‑1 (MQ=255)                               acaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                        <  1:851120/140‑1 (MQ=255)                               acaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                        <  1:990841/140‑1 (MQ=255)                                 aTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGAtt                                                                                                      <  2:269333/140‑1 (MQ=255)                                  tCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGAttt                                                                                                     >  1:559473/1‑140 (MQ=255)                                     caGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCaa                                                                                                  <  2:632571/140‑1 (MQ=255)                                      aGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAAt                                                                                                 >  2:290836/1‑140 (MQ=255)                                                 tGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGc                                                                                      <  1:1067717/140‑1 (MQ=255)                                                       aTAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCaa                                                                                >  2:7860/1‑140 (MQ=255)                                                         aaTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCaaaa                                                                              >  1:700585/1‑140 (MQ=255)                                                            tGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                           <  1:590784/140‑1 (MQ=255)                                                            tGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                           <  1:782172/140‑1 (MQ=255)                                                            tGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                           <  2:700585/140‑1 (MQ=255)                                                            tGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                           <  1:1104784/140‑1 (MQ=255)                                                                   aaaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCt                                                                    >  2:874269/1‑140 (MQ=255)                                                                    aaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTg                                                                   <  1:874269/140‑1 (MQ=255)                                                                      aGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTggg                                                                 <  1:973070/140‑1 (MQ=255)                                                                             aaaGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATAcc                                                          <  2:49305/140‑1 (MQ=255)                                                                                 tttGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACg                                                      >  2:1039656/1‑140 (MQ=255)                                                                                  ttGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGc                                                     >  2:920450/1‑140 (MQ=255)                                                                                  ttGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGc                                                     <  2:598141/140‑1 (MQ=255)                                                                                                acaAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTgccagc                                       <  2:243843/140‑1 (MQ=255)                                                                                                acaAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTgccagc                                       <  1:1039656/140‑1 (MQ=255)                                                                                                   aaCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGccagccag                                    <  2:559473/140‑1 (MQ=255)                                                                                                              tttAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTgccagc                                       <  1:266796/126‑1 (MQ=255)                                                                                                              tttAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTgccagc                                       >  2:266796/1‑126 (MQ=255)                                                                                                              tttAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGTCAGTCGCGTTGTGc                         >  2:777219/1‑140 (MQ=255)                                                                                                              tttAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTtg                            <  1:955929/137‑1 (MQ=255)                                                                                                              tttAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTtg                            >  2:955929/1‑137 (MQ=255)                                                                                                              tttAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGc                         >  2:344857/1‑140 (MQ=255)                                                                                                              tttAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGc                         >  2:189513/1‑140 (MQ=255)                                                                                                              tttAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGc                         >  1:1205602/1‑140 (MQ=255)                                                                                                               ttAAAGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCt                        <  1:613434/140‑1 (MQ=255)                                                                                                                  aaGTTTTGTAATCAGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCa                     <  1:718052/140‑1 (MQ=255)                                                                                                                               aGTTTGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCAGTCTCCACGCCtt        >  2:674393/1‑140 (MQ=255)                                                                                                                                 tttGGGGCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCAGTCTCCACGCCTTCg      <  2:1205602/140‑1 (MQ=255)                                                                                                                                     gggCAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCAGTCTCCACGCCTTCGGCaa  <  1:7860/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCAGTCTCCACGCCTTCGGCAA  >  NC_000913/3683492‑3683763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑