Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,935,010	G→A	M411I (ATG→ATA)	rbsA →	ribose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,935,010	0	G	A	100.0%	250.1 / NA	70	M411I (ATG→ATA)	rbsA	ribose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/38);  total (32/38)

TGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAG  >  NC_000913/3934874‑3935146                                                                                                                                         |                                                                                                                                         tGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAaca                                                                                                                                       <  2:454869/140‑1 (MQ=255)        tGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCa                                                                                                                                >  1:435026/1‑140 (MQ=255)        tGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGT‑GCGATTGCCCGCGGTCTGATAACACGCCCCaa                                                                                                                               >  2:1181063/1‑140 (MQ=255)         gCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCaa                                                                                                                               <  2:435026/140‑1 (MQ=255)         gCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCaa                                                                                                                               >  1:983092/1‑140 (MQ=255)                        gCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTtga                                                                                                                >  1:203031/1‑140 (MQ=255)                        gCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTtga                                                                                                                >  1:449663/1‑140 (MQ=255)                          tgtgAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGtt                                                                                                                         >  2:1140506/1‑129 (MQ=255)                          tgtgAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGtt                                                                                                                         <  1:1140506/129‑1 (MQ=255)                          tgtgAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTtgatg                                                                                                              <  2:803621/140‑1 (MQ=255)                          tgtgAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTtgatg                                                                                                              >  1:455326/1‑140 (MQ=255)                          tgtgAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTtgatg                                                                                                              >  1:93695/1‑140 (MQ=255)                            tgagtgaTTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGaa                                                                                                            >  2:662301/1‑140 (MQ=255)                              agtgaTTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAAcc                                                                                                          <  2:498459/140‑1 (MQ=255)                                 gaTTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTAc                                                                                                       <  2:203031/140‑1 (MQ=255)                                  aTTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTAcc                                                                                                      >  1:96515/1‑140 (MQ=255)                                  aTTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTAcc                                                                                                      >  2:533301/1‑140 (MQ=255)                                   tttCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTAccc                                                                                                     >  2:512104/1‑140 (MQ=255)                                    ttCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCg                                                                                                    <  1:533301/140‑1 (MQ=255)                                      cATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTg                                                                                                  >  1:653961/1‑140 (MQ=255)                                      cATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTg                                                                                                  >  1:523068/1‑140 (MQ=255)                                              tGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTAccc                                                                                                     <  1:1103050/129‑1 (MQ=255)                                              tGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCACGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTAccc                                                                                                     >  2:1103050/1‑129 (MQ=255)                                                     tgtgAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGgcg                                                                                   >  2:190402/1‑140 (MQ=255)                                                     tgtgAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGgcg                                                                                   >  1:1132684/1‑140 (MQ=255)                                                          aaaCTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGaaa                                                                              >  1:334393/1‑140 (MQ=255)                                                          aaaCTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGaaa                                                                              >  2:483832/1‑140 (MQ=255)                                                          aaaCTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGaaa                                                                              >  2:925974/1‑140 (MQ=255)                                                             cTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGaaaaaa                                                                           <  2:523068/140‑1 (MQ=255)                                                             cTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGG‑‑TGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAaga                                                                         <  1:353675/140‑1 (MQ=255)                                                               ccGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAaga                                                                         <  2:444179/140‑1 (MQ=255)                                                               ccGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAaga                                                                         <  2:238038/140‑1 (MQ=255)                                                               ccGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAaga                                                                         >  1:1039299/1‑140 (MQ=255)                                                               ccGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAaga                                                                         <  1:995472/140‑1 (MQ=255)                                                               ccGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAaga                                                                         <  1:249181/140‑1 (MQ=255)                                                               ccGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAaga                                                                         <  1:925974/140‑1 (MQ=255)                                                                cGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAagag                                                                        >  2:958429/1‑140 (MQ=255)                                                                 gTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAaga                                                                         >  2:11395/1‑138 (MQ=255)                                                                 gTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAaga                                                                         <  1:11395/138‑1 (MQ=255)                                                                 gTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAgaga                                                                       >  2:947173/1‑140 (MQ=255)                                                                 gTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAgaga                                                                       >  2:651003/1‑140 (MQ=255)                                                                        gAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCa                                                                <  2:684271/140‑1 (MQ=255)                                                                        gAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCa                                                                <  2:983092/140‑1 (MQ=255)                                                                        gAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCa                                                                <  1:190402/140‑1 (MQ=255)                                                                          aCAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGGTCTATCAAc                                                              <  1:90222/140‑1 (MQ=255)                                                                             ggCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGa                                                           <  1:717681/140‑1 (MQ=255)                                                                                aaTTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCa                                                                <  2:712943/132‑1 (MQ=255)                                                                                aaTTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCa                                                                >  1:712943/1‑132 (MQ=255)                                                                                        tGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTc                                                >  1:1165241/1‑140 (MQ=255)                                                                                        tGCTTTCCGGTGGCAATCAGCAAAAAGT‑GCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCa                                               <  1:1181063/140‑1 (MQ=255)                                                                                         gCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCa                                               <  2:653961/140‑1 (MQ=255)                                                                                          cTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCaa                                              <  1:483832/140‑1 (MQ=255)                                                                                            ttCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAg                                            <  2:648593/140‑1 (MQ=255)                                                                                            ttCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAg                                            <  2:455326/140‑1 (MQ=255)                                                                                             tCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGc                                           >  2:501359/1‑140 (MQ=255)                                                                                             tCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGc                                           >  2:856015/1‑140 (MQ=255)                                                                                               cGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCg                                         <  1:947173/140‑1 (MQ=255)                                                                                                    gCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGc                                    >  2:535327/1‑140 (MQ=255)                                                                                                      aaTCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCtt                                  <  2:1132684/140‑1 (MQ=255)                                                                                                      aaTCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCtt                                  <  2:713204/140‑1 (MQ=255)                                                                                                       aTCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTg                                 <  2:1165241/140‑1 (MQ=255)                                                                                                                aaGTGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCAtt                        <  2:93695/140‑1 (MQ=255)                                                                                                                   tGGCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTg                     <  2:334393/140‑1 (MQ=255)                                                                                                                     gCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGgt                   <  1:501359/140‑1 (MQ=255)                                                                                                                     gCGATTGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGgt                   <  2:449663/140‑1 (MQ=255)                                                                                                                          tGCCCGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCAt              >  1:87175/1‑140 (MQ=255)                                                                                                                             ccGCGGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCgg           >  1:86453/1‑140 (MQ=255)                                                                                                                              cgcgGTCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGga          <  1:525382/140‑1 (MQ=255)                                                                                                                                   tCTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGc     <  2:936599/140‑1 (MQ=255)                                                                                                                                    cTGATAACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGcc    <  2:519553/140‑1 (MQ=255)                                                                                                                                      gataaCACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAg  <  1:856015/140‑1 (MQ=255)                                                                                                                                      gataaCACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAg  <  1:958429/140‑1 (MQ=255)                                                                                                                                      gataaCACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTAGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAg  >  1:309814/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TGAAGCATGCCGATGAACAGCAGGCTGTGAGTGATTTCATTCGTCTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAG  >  NC_000913/3934874‑3935146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑