Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,445,549	A→G	T480A (ACC→GCC)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,445,549	0	A	G	100.0%	301.9 / NA	82	T480A (ACC→GCC)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/45);  total (37/45)

CGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAA  >  NC_000913/4445419‑4445685                                                                                                                                   |                                                                                                                                         cGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGa                                                                                                                                 <  2:164714/140‑1 (MQ=255)     tGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGa                                                                                                                             <  1:192427/140‑1 (MQ=255)       gCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACg                                                                                                                           >  2:1147029/1‑140 (MQ=255)       gCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACg                                                                                                                           <  2:263298/140‑1 (MQ=255)              gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGcc                                                                                                                       >  1:381908/1‑137 (MQ=255)              gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGcc                                                                                                                       <  2:381908/137‑1 (MQ=255)              gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGa                                                                                                                    >  1:279421/1‑140 (MQ=255)                 aTTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGtt                                                                                                                 >  2:331386/1‑140 (MQ=255)                     gTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAg                                                                                                             <  2:74396/140‑1 (MQ=255)                        ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCt                                                                                                           >  1:665681/1‑139 (MQ=255)                        ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCt                                                                                                           <  2:665681/139‑1 (MQ=255)                        ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTg                                                                                                          >  1:728565/1‑140 (MQ=255)                        ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTg                                                                                                          >  1:923125/1‑140 (MQ=255)                             ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  1:253362/140‑1 (MQ=255)                              gTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  2:840625/139‑1 (MQ=255)                              gTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     >  1:840625/1‑139 (MQ=255)                              gTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGt                                                                                                    <  1:190079/140‑1 (MQ=255)                                  gCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACg                                                                                                <  2:946004/140‑1 (MQ=255)                                      aCGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTaa                                                                                            >  2:378596/1‑140 (MQ=255)                                       cGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     >  1:419171/1‑130 (MQ=255)                                       cGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  2:419171/130‑1 (MQ=255)                                         cTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACa                                                                                         >  1:487796/1‑140 (MQ=255)                                           tAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGTTGGCCGTGCCGGAGTTGAAGCTGACCGGTAACGATACACAGa                                                                                       >  2:774570/1‑140 (MQ=255)                                           tAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGa                                                                                       >  1:1049273/1‑140 (MQ=255)                                            accGGCATTAACACCGCCAAAGAGTACCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAACCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGaa                                                                                      <  1:774570/138‑1 (MQ=255)                                            aaCGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGaa                                                                                      >  1:510008/1‑140 (MQ=255)                                            aaCGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGaa                                                                                      <  1:153889/140‑1 (MQ=255)                                             aCGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACTGGTAACGTTAAACAGAAc                                                                                     >  2:1038120/1‑140 (MQ=255)                                                 cATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAg                                                                                 >  2:827820/1‑140 (MQ=255)                                                 cATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAg                                                                                 <  2:923125/140‑1 (MQ=255)                                                    tAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGa                                                                              <  2:423800/140‑1 (MQ=255)                                                    tAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGa                                                                              <  2:775428/140‑1 (MQ=255)                                                    tAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGa                                                                              >  1:1010085/1‑140 (MQ=255)                                                         ccGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGtt                                                                         >  1:800569/1‑140 (MQ=255)                                                           gCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGa                                                                       >  1:866205/1‑140 (MQ=255)                                                           gCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGa                                                                       >  1:1125040/1‑140 (MQ=255)                                                                agagTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCa                                                                  >  1:1063390/1‑140 (MQ=255)                                                                    ttCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCt                                                              >  1:245963/1‑140 (MQ=255)                                                                        cGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGaaa                                                          <  2:800569/140‑1 (MQ=255)                                                                           aCTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                       <  2:1125040/140‑1 (MQ=255)                                                                            cTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCa                                                      >  2:509393/1‑140 (MQ=255)                                                                             tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  2:1010085/140‑1 (MQ=255)                                                                             tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  2:245963/140‑1 (MQ=255)                                                                             tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  2:866205/140‑1 (MQ=255)                                                                             tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  2:510008/140‑1 (MQ=255)                                                                             tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  2:729033/140‑1 (MQ=255)                                                                              ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGa                                                            >  2:526020/1‑132 (MQ=255)                                                                              ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGa                                                            <  1:526020/132‑1 (MQ=255)                                                                              ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     >  2:915061/1‑139 (MQ=255)                                                                              ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:915061/139‑1 (MQ=255)                                                                              ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAAc                                                    >  2:153641/1‑140 (MQ=255)                                                                              ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAAc                                                    >  1:1204894/1‑140 (MQ=255)                                                                              ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAAc                                                    >  2:272889/1‑140 (MQ=255)                                                                               gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGGGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACa                                                   >  2:775160/1‑140 (MQ=255)                                                                               gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACa                                                   >  2:455607/1‑140 (MQ=255)                                                                               gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACa                                                   >  1:1132728/1‑140 (MQ=255)                                                                                   tCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTta                                               >  1:198920/1‑140 (MQ=255)                                                                                    cGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGGGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTtat                                              <  1:775160/140‑1 (MQ=255)                                                                                     gAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:1168484/132‑1 (MQ=255)                                                                                     gAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     >  2:1168484/1‑132 (MQ=255)                                                                                     gAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTtata                                             <  1:608765/140‑1 (MQ=255)                                                                                     gAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTtata                                             <  2:464701/140‑1 (MQ=255)                                                                                       aaCTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATg                                           <  1:827820/140‑1 (MQ=255)                                                                                         cTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCa                                         >  2:1201630/1‑140 (MQ=255)                                                                                          tCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAg                                        <  1:153641/140‑1 (MQ=255)                                                                                             aTGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTgg                                     <  1:455607/140‑1 (MQ=255)                                                                                               ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACTGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  2:279421/140‑1 (MQ=255)                                                                                               ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  2:902140/140‑1 (MQ=255)                                                                                               ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  1:272889/140‑1 (MQ=255)                                                                                               ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  2:728565/140‑1 (MQ=255)                                                                                                   tGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATc                               >  2:518436/1‑140 (MQ=255)                                                                                                    gcttAAAACCCGCGGTACCCTGTACGGCCGCGCCTGGCAGATGGACGTGCCGGAGATGAAGCGGACCGGAAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATcc                              >  2:872597/3‑140 (MQ=255)                                                                                                    gATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATcc                              <  2:1132728/140‑1 (MQ=255)                                                                                                     aTTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATccc                             <  2:649438/140‑1 (MQ=255)                                                                                                      ttAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCa                            <  1:213883/140‑1 (MQ=255)                                                                                                      ttAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCa                            >  1:887661/1‑140 (MQ=255)                                                                                                          aaCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGc                        <  2:487796/140‑1 (MQ=255)                                                                                                             ccGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTc                     <  2:1049273/140‑1 (MQ=255)                                                                                                               gcgGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCAt                   >  1:596766/1‑140 (MQ=255)                                                                                                               gcgGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCAt                   >  1:955438/1‑140 (MQ=255)                                                                                                                      ccTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  2:1045987/140‑1 (MQ=255)                                                                                                                      ccTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  1:378596/140‑1 (MQ=255)                                                                                                                      ccTGTACGGCGGCGCCTGGCAGATGGACGTGCCGAAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  1:1147029/140‑1 (MQ=255)                                                                                                                                ggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACTGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCCTCATCTGGAACTCGGGCCaaa  <  1:1038120/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         CGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAA  >  NC_000913/4445419‑4445685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑