Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,518,178	G→A	H20Y (CAT→TAT)	fecI ←	RNA polymerase sigma factor FecI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,518,178	0	G	A	100.0%	320.5 / NA	88	H20Y (CAT→TAT)	fecI	RNA polymerase sigma factor FecI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/52);  total (36/52)

AGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAAT  >  NC_000913/4518042‑4518300                                                                                                                                         |                                                                                                                           aggaaggaGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGAtatgt                                                                                                                         >  1:216940/1‑140 (MQ=255)   gaaggaGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTAACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGc                                                                                                                       >  2:790012/1‑140 (MQ=255)      ggaGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCAt                                                                                                                    >  1:1049043/1‑140 (MQ=255)      ggaGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCAt                                                                                                                    >  1:1142079/1‑140 (MQ=255)      ggaGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCAt                                                                                                                    >  1:532168/1‑140 (MQ=255)        aGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATaa                                                                                                                  >  2:988173/1‑140 (MQ=255)        aGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATaa                                                                                                                  >  2:541432/1‑140 (MQ=255)           gAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGc                                                                                                               >  1:639738/1‑140 (MQ=255)             ggATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGa                                                                                                             >  1:118387/1‑140 (MQ=255)             ggATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGa                                                                                                             >  2:125414/1‑140 (MQ=255)             ggATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGa                                                                                                             <  2:639738/140‑1 (MQ=255)               aTCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACt                                                                                                           <  2:413432/140‑1 (MQ=255)                tCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTc                                                                                                          <  2:216940/140‑1 (MQ=255)                tCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTc                                                                                                          <  1:64601/140‑1 (MQ=255)                tCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTc                                                                                                          <  1:586973/140‑1 (MQ=255)                 cgcgGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCg                                                                                                         <  1:541432/140‑1 (MQ=255)                 cgcgGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCg                                                                                                         >  1:1133778/1‑140 (MQ=255)                 cgcgGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCg                                                                                                         <  1:624891/140‑1 (MQ=255)                  gcgGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGTAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGa                                                                                                        <  1:792242/140‑1 (MQ=255)                  gcgGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGa                                                                                                        >  2:611670/1‑140 (MQ=255)                  gcgGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGa                                                                                                        >  2:542646/1‑140 (MQ=255)                  gcgGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGa                                                                                                        <  2:100327/140‑1 (MQ=255)                        cgtcgAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGaa                                                                                                       >  1:1154160/1‑135 (MQ=255)                        cgtcgAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGaa                                                                                                       <  2:1154160/135‑1 (MQ=255)                        cgtcgAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTa                                                                                                  <  2:532168/140‑1 (MQ=255)                         gtcgAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTaa                                                                                                 <  1:385779/140‑1 (MQ=255)                          tcgAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAg                                                                                                <  1:350446/140‑1 (MQ=255)                          tcgAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAg                                                                                                <  2:1049043/140‑1 (MQ=255)                          tcgAGAGCGTTTCGCTAACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAg                                                                                                <  1:790012/140‑1 (MQ=255)                           cgAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAg                                                                                                >  2:174025/1‑139 (MQ=255)                           cgAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAg                                                                                                <  1:174025/139‑1 (MQ=255)                            gagaGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGa                                                                                              <  1:542646/140‑1 (MQ=255)                            gagaGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGa                                                                                              <  1:1180413/140‑1 (MQ=255)                            gagaGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCCTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGa                                                                                              <  2:334238/140‑1 (MQ=255)                             agagCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGaa                                                                                             <  1:627895/140‑1 (MQ=255)                              gagCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAg                                                                                                >  2:518556/1‑136 (MQ=255)                              gagCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAg                                                                                                <  1:518556/136‑1 (MQ=255)                              gagCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAg                                                                                            <  2:75207/140‑1 (MQ=255)                                 cGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCtg                                                                                         <  1:660307/140‑1 (MQ=255)                                  gTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGa                                                                                              <  2:153328/134‑1 (MQ=255)                                  gTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGa                                                                                              >  1:153328/1‑134 (MQ=255)                                   tttCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAgg                                                                                               >  2:489532/1‑132 (MQ=255)                                   tttCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAgg                                                                                               <  1:489532/132‑1 (MQ=255)                                   tttCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCtgtg                                                                                       >  1:739419/1‑140 (MQ=255)                                           aCCATTACCCGCAAAAAAGTGTTCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGgc                                                                               <  1:872553/140‑1 (MQ=255)                                           aCCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGgc                                                                               <  2:714701/140‑1 (MQ=255)                                             cATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGgcgc                                                                             <  1:1147939/140‑1 (MQ=255)                                                tACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGt                                                                          >  2:1132681/1‑140 (MQ=255)                                                     gCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGAc                                                                     >  2:840222/1‑140 (MQ=255)                                                     gCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGAc                                                                     <  1:417343/140‑1 (MQ=255)                                                     gCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGAc                                                                     >  2:208485/1‑140 (MQ=255)                                                     gCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGAc                                                                     >  1:29844/1‑140 (MQ=255)                                                          aaaGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCg                                                                <  1:810955/140‑1 (MQ=255)                                                             gtgtCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAg                                                             <  2:883721/140‑1 (MQ=255)                                                             gtgtCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAg                                                             >  2:129096/1‑140 (MQ=255)                                                                   tGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATc                                                       >  1:443469/1‑140 (MQ=255)                                                                     ggCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCg                                                                <  1:1045149/129‑1 (MQ=255)                                                                     ggCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCg                                                                >  2:1045149/1‑129 (MQ=255)                                                                     ggCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCaa                                                     <  1:988173/140‑1 (MQ=255)                                                                     ggCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCaa                                                     <  1:208485/140‑1 (MQ=255)                                                                          tGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCg                                                                >  2:302322/1‑124 (MQ=255)                                                                          tGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCg                                                                <  1:302322/124‑1 (MQ=255)                                                                          tGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGtt                                                >  1:1200236/1‑140 (MQ=255)                                                                          tGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGtt                                                >  2:1920/1‑140 (MQ=255)                                                                          tGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGtt                                                >  2:679469/1‑140 (MQ=255)                                                                            tCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTaa                                              >  2:582870/1‑140 (MQ=255)                                                                              aTCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAAtt                                            <  1:1920/140‑1 (MQ=255)                                                                                  gCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCa                                        <  1:230768/140‑1 (MQ=255)                                                                                  gCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCa                                        <  2:922280/140‑1 (MQ=255)                                                                                    aTCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACg                                      >  2:521780/1‑140 (MQ=255)                                                                                    aTCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACg                                      <  1:1132681/140‑1 (MQ=255)                                                                                    aTCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACg                                      <  2:739419/140‑1 (MQ=255)                                                                                      cAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTa                                    <  2:456373/140‑1 (MQ=255)                                                                                       aaaaGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAg                                   <  1:611670/140‑1 (MQ=255)                                                                                       aaaaGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTAAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAg                                   <  1:126522/140‑1 (MQ=255)                                                                                         aaGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTc                                 <  1:521780/140‑1 (MQ=255)                                                                                         aaGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTc                                 <  1:204139/140‑1 (MQ=255)                                                                                            cAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCata                              <  1:85308/140‑1 (MQ=255)                                                                                             aGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCatat                             >  1:1214941/1‑140 (MQ=255)                                                                                                cTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTaa                          <  2:1142079/140‑1 (MQ=255)                                                                                                      tttGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATgag                    <  2:1200236/140‑1 (MQ=255)                                                                                                       ttGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATgaga                   <  2:443469/140‑1 (MQ=255)                                                                                                         gcgcGTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAAt                 >  2:571707/1‑140 (MQ=255)                                                                                                          cgcgTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATg                >  2:1204944/1‑140 (MQ=255)                                                                                                            cgTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGt              <  1:129096/140‑1 (MQ=255)                                                                                                             gTCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGtt             <  2:831557/140‑1 (MQ=255)                                                                                                              tCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTa            >  2:832635/1‑140 (MQ=255)                                                                                                              tCAGCCAGCTTTTCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTa            <  1:370103/140‑1 (MQ=255)                                                                                                                        tttCAACCAGCCGTGATATGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAAt  >  2:1127577/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                           AGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAAT  >  NC_000913/4518042‑4518300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑