Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,685	A→G	intergenic (‑163/‑48)	ettA ← / → slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,685	0	A	G	100.0%	257.3 / NA	69	intergenic (‑163/‑48)	ettA/slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/40);  total (29/40)

GATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTC  >  NC_000913/4630550‑4630818                                                                                                                                        |                                                                                                                                      gATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGcgcg                                                                                                                                   >  1:962403/1‑140 (MQ=255) gATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGcgcg                                                                                                                                   >  2:542964/1‑140 (MQ=255)  aTAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCgg                                                                                                                                  >  2:368543/1‑140 (MQ=255)  aTAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCgg                                                                                                                                  >  1:192345/1‑140 (MQ=255)   tAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGc                                                                                                                                 >  1:842982/1‑140 (MQ=255)    aaTGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCa                                                                                                                                <  2:164504/140‑1 (MQ=255)    aaTGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCa                                                                                                                                <  1:1023841/140‑1 (MQ=255)    aaTGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCa                                                                                                                                <  1:526578/140‑1 (MQ=255)     aTGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCAt                                                                                                                               >  1:734391/1‑140 (MQ=255)       gAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGa                                                                                                                             <  2:573632/140‑1 (MQ=255)       gAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGa                                                                                                                             <  1:368543/140‑1 (MQ=255)         gTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACg                                                                                                                           <  1:37349/140‑1 (MQ=255)           tttCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCt                                                                                                                         <  1:475903/140‑1 (MQ=255)            ttCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTg                                                                                                                        <  2:962403/140‑1 (MQ=255)             tCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCTCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGc                                                                                                                       >  2:465732/1‑140 (MQ=255)             tCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCa                                                                                                                                >  2:972191/1‑131 (MQ=255)             tCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCa                                                                                                                                <  1:972191/131‑1 (MQ=255)              cAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCa                                                                                                                      <  2:525940/140‑1 (MQ=255)              cAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCa                                                                                                                      <  2:319920/140‑1 (MQ=255)              cAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCa                                                                                                                      <  1:454899/140‑1 (MQ=255)              cAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCa                                                                                                                      <  1:430801/140‑1 (MQ=255)                  ggCGAAGTGTAGCCTTTTTCCCTGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGa                                                                                                                  <  2:53454/140‑1 (MQ=255)                  ggCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGa                                                                                                                  <  2:60853/140‑1 (MQ=255)                  ggCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGa                                                                                                                  <  1:542964/140‑1 (MQ=255)                    cGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGT‑AAAGTAAAAAAGTGTCCGTAACGTGGCGTGAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGt                                                                                                               >  2:501124/1‑140 (MQ=255)                       aGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTAt                                                                                                             <  1:109811/140‑1 (MQ=255)                       aGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTAt                                                                                                             >  1:700391/1‑140 (MQ=255)                        gtgtAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTAtt                                                                                                            <  1:153896/140‑1 (MQ=255)                          gtAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTa                                                                                                          <  2:700391/140‑1 (MQ=255)                          gtAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTa                                                                                                          <  1:37272/140‑1 (MQ=255)                          gtAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTa                                                                                                          <  2:1008722/140‑1 (MQ=255)                              ccTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACt                                                                                                      <  1:784021/140‑1 (MQ=255)                                  tttCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTa                                                                                                          >  2:429734/1‑132 (MQ=255)                                  tttCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTa                                                                                                          <  1:429734/132‑1 (MQ=255)                                   ttCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTAttt                                                                                                           <  1:307258/130‑1 (MQ=255)                                   ttCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTAttt                                                                                                           >  2:307258/1‑130 (MQ=255)                                     ccccGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGa                                                                                               <  2:68040/140‑1 (MQ=255)                                          cccaaTCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATgcgc                                                                                          <  1:1038951/137‑1 (MQ=255)                                                    gCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGaaaa                                                                                >  1:311927/1‑140 (MQ=255)                                                     ccGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGTAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGaaaaa                                                                               >  1:420233/1‑140 (MQ=255)                                                         tCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCa                                                                           >  1:395782/1‑140 (MQ=255)                                                         tCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCa                                                                           >  2:649098/1‑140 (MQ=255)                                                         tCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCa                                                                           >  2:42809/1‑140 (MQ=255)                                                           gATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCaaa                                                                         >  2:1235122/1‑140 (MQ=255)                                                                  cTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTa                                                                  <  1:1039952/140‑1 (MQ=255)                                                                   tCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTAc                                                                 >  1:244497/1‑140 (MQ=255)                                                                     gCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCt                                                               <  2:192345/140‑1 (MQ=255)                                                                      cAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTg                                                              >  1:860992/1‑140 (MQ=255)                                                                             agtaaaaAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGTATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTg                                                       <  2:842982/140‑1 (MQ=255)                                                                                 aaaaaGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTgg                                                   <  2:860992/140‑1 (MQ=255)                                                                                  aaaaGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGc                                                  <  2:1083003/140‑1 (MQ=255)                                                                                     aGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGc                                               <  1:649098/140‑1 (MQ=255)                                                                                       tgtCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCg                                             >  2:810929/1‑140 (MQ=255)                                                                                         tCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGgt                                           >  1:450878/1‑140 (MQ=255)                                                                                         tCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGgt                                           <  1:1028330/140‑1 (MQ=255)                                                                                         tCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGgt                                           >  1:239159/1‑140 (MQ=255)                                                                                         tCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGgt                                           >  1:502064/1‑140 (MQ=255)                                                                                          ccGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGgtg                                          <  1:42809/140‑1 (MQ=255)                                                                                                   ggCGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTctgc                                 <  1:1235122/140‑1 (MQ=255)                                                                                                     cGTAAACGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAACTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTctgctg                               <  2:395782/140‑1 (MQ=255)                                                                                                          aCGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGgtgt                                         <  2:1231321/125‑1 (MQ=255)                                                                                                          aCGGCAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGgtgt                                         >  1:1231321/1‑125 (MQ=255)                                                                                                              cAATGACTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTcagc                      >  2:161165/1‑140 (MQ=255)                                                                                                                   aCTGGTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGt                 >  2:499961/1‑140 (MQ=255)                                                                                                                      ggTTAGCATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGgc              >  2:678056/1‑140 (MQ=255)                                                                                                                            cATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGc        <  1:501124/140‑1 (MQ=255)                                                                                                                            cATAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGc        <  2:244497/140‑1 (MQ=255)                                                                                                                             aTAAATCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGcc       >  1:656239/1‑140 (MQ=255)                                                                                                                                  tCTATTGCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTc  <  2:930225/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTC  >  NC_000913/4630550‑4630818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑