Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,892	A→G	S8G (AGC→GGC)	mdlA →	ABC transporter family protein MdlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,892	0	A	G	100.0%	311.8 / NA	83	S8G (AGC→GGC)	mdlA	ABC transporter family protein MdlA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/38);  total (45/38)

AACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAGAACAACACTTTACTA  >  NC_000913/468758‑469027                                                                                                                                       |                                                                                                                                         aaCAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTgg                                                                                                                                     >  1:911305/1‑140 (MQ=255)   cAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTa                                                                                                                                   <  1:412214/140‑1 (MQ=255)      cGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCt                                                                                                                                       <  2:23330/133‑1 (MQ=255)      cGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCt                                                                                                                                       >  1:23330/1‑133 (MQ=255)      cGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTAttt                                                                                                                                >  1:804862/1‑140 (MQ=255)      cGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTAttt                                                                                                                                >  1:166618/1‑140 (MQ=255)      cGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTAttt                                                                                                                                <  2:804862/140‑1 (MQ=255)      cGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTAttt                                                                                                                                >  1:1020200/1‑140 (MQ=255)        tGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTcc                                                                                                                              >  2:1096867/1‑140 (MQ=255)                     ggACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCg                                                                                                                 <  2:473515/140‑1 (MQ=255)                                 cAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCg‑g                                                                                                    >  2:681488/1‑140 (MQ=255)                                   gCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCg‑ggg                                                                                                  <  2:1020200/140‑1 (MQ=255)                                    cTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGc                                                                                                 >  1:467989/1‑140 (MQ=255)                                       cgcgATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGt                                                                                              >  2:58332/1‑140 (MQ=255)                                       cgcgATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGt                                                                                              >  2:846244/1‑140 (MQ=255)                                       cgcgATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGt                                                                                              >  2:771801/1‑140 (MQ=255)                                        gcgATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTc                                                                                             >  1:419887/1‑140 (MQ=255)                                         cgATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCg                                                                                            <  1:58815/140‑1 (MQ=255)                                            tGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCct                                                                                         <  2:467989/140‑1 (MQ=255)                                                aCAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCcttgct                                                                                     <  2:641037/140‑1 (MQ=255)                                                aCAGATTAAATACACCACTTCTTTACCCATCGAATAAACATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCcttgct                                                                                     <  1:249849/140‑1 (MQ=255)                                                    aTTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCct                                                                                         <  2:503277/132‑1 (MQ=255)                                                    aTTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCct                                                                                         >  1:503277/1‑132 (MQ=255)                                                           acacCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATcg                                                                          <  1:1096867/140‑1 (MQ=255)                                                            caccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATcgc                                                                         <  2:259131/140‑1 (MQ=255)                                                            caccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATcgc                                                                         >  2:613152/1‑140 (MQ=255)                                                             accacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATcgcg                                                                        >  2:46572/1‑140 (MQ=255)                                                             accacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATcgcg                                                                        >  1:271421/1‑140 (MQ=255)                                                              ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCcttgc                                                                                      <  2:1055570/125‑1 (MQ=255)                                                              ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCcttgc                                                                                      >  1:1055570/1‑125 (MQ=255)                                                              ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGa                                                                       >  2:686110/1‑140 (MQ=255)                                                              ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGa                                                                       >  1:295805/1‑140 (MQ=255)                                                              ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGa                                                                       >  1:362251/1‑140 (MQ=255)                                                              ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGa                                                                       >  1:729364/1‑140 (MQ=255)                                                              ccacTTCGTTACCCATCGAATAAATATCCAGACTCACGTCAGGACACAACCCGTGCGATTATTTGCTCAACTAGGCTGGTATCCCCGTCGGGAATGGCGTCCCTATCTCG‑GCGCTGTCGCCTTGCTTGTCATTATCGCGa                                                                       <  2:732376/140‑1 (MQ=255)                                                                acTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGAtg                                                                     <  2:295805/140‑1 (MQ=255)                                                                acTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGAtg                                                                     <  2:166618/140‑1 (MQ=255)                                                                    cttTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGTGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGAtgctgc                                                                 >  2:945466/1‑140 (MQ=255)                                                                    cttTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGAtgctgc                                                                 >  1:47738/1‑140 (MQ=255)                                                                            aTCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGtt                                                         >  1:479352/1‑140 (MQ=255)                                                                            aTCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGtt                                                         >  1:1173467/1‑140 (MQ=255)                                                                            aTCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGtt                                                         >  2:797935/1‑140 (MQ=255)                                                                            aTCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGtt                                                         >  1:934412/1‑140 (MQ=255)                                                                              cGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGAt                                                                      <  1:281727/125‑1 (MQ=255)                                                                              cGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGAt                                                                      >  2:281727/1‑125 (MQ=255)                                                                              cGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTcc                                                       >  1:145348/1‑140 (MQ=255)                                                                                     atatCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCaaaa                                                <  1:613152/140‑1 (MQ=255)                                                                                       atCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGt                                              <  1:686110/140‑1 (MQ=255)                                                                                        tCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGtg                                             <  2:271421/140‑1 (MQ=255)                                                                                          cAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGtggt                                           <  1:797935/140‑1 (MQ=255)                                                                                             aaTCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGtggttgg                                        <  2:729364/140‑1 (MQ=255)                                                                                               tcaggtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTa                                      <  1:46572/140‑1 (MQ=255)                                                                                               tcaggtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTa                                      <  1:771801/140‑1 (MQ=255)                                                                                               tcaggtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTACGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTa                                      >  2:341388/1‑140 (MQ=255)                                                                                                 aggtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTAtt                                    <  2:419887/140‑1 (MQ=255)                                                                                                   gtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGtggt                                           <  2:894215/131‑1 (MQ=255)                                                                                                   gtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGtggt                                           >  1:894215/1‑131 (MQ=255)                                                                                                   gtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTAttgt                                  >  2:919557/1‑140 (MQ=255)                                                                                                   gtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTAttgt                                  >  1:740983/1‑140 (MQ=255)                                                                                                   gtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTAttgt                                  >  2:562771/1‑140 (MQ=255)                                                                                                   gtcaggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTAttgt                                  >  2:283403/1‑140 (MQ=255)                                                                                                       ggACACAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTc                              <  1:681488/140‑1 (MQ=255)                                                                                                         acacAACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGa                            <  2:934412/140‑1 (MQ=255)                                                                                                            caACGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATgg                         <  2:595345/140‑1 (MQ=255)                                                                                                             aaCGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGc                        >  2:151427/1‑140 (MQ=255)                                                                                                             aaCGCGTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGc                        >  2:834926/1‑140 (MQ=255)                                                                                                             aaCGCGTGCGATTATTTGCTAAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGc                        >  2:158700/1‑140 (MQ=255)                                                                                                             aaCGCGTG‑GATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTc                              >  1:721383/1‑133 (MQ=255)                                                                                                             aaCGCGTG‑GATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTc                              <  2:721383/133‑1 (MQ=255)                                                                                                               cgcgTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGt                      >  2:337609/1‑140 (MQ=255)                                                                                                                 cgTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGa                    >  1:1233458/1‑140 (MQ=255)                                                                                                                 cgTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGa                    <  2:740983/140‑1 (MQ=255)                                                                                                                  gTGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGAc                   >  1:515419/1‑140 (MQ=255)                                                                                                                   tGCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACa                  >  2:1087800/1‑140 (MQ=255)                                                                                                                    gCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAg                 <  2:1173467/140‑1 (MQ=255)                                                                                                                    gCGATTATTTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAg                 <  1:58332/140‑1 (MQ=255)                                                                                                                         tattTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAGaacaa            <  1:170996/140‑1 (MQ=255)                                                                                                                         tattTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAGaacaa            <  2:1233458/140‑1 (MQ=255)                                                                                                                         tattTGCTCAATTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAGaacaa            <  2:479352/140‑1 (MQ=255)                                                                                                                         tattTGCTCAATTAGGCTGGTATTTACGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAGaacaa            <  1:341388/140‑1 (MQ=255)                                                                                                                                  aaTTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGTATGCCGTCTTC‑TGCTTGAAAAAAAAAAGATGCTGCAACTGGTTCCGCCAAAAGGGGTTGGGATTGTTGTCGATGGCGTGACAGAACAACACTTtact   >  1:776200/1‑140 (MQ=255)                                                                                                                                  aaTTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAGaa               <  1:5277/128‑1 (MQ=255)                                                                                                                                  aaTTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAGaa               >  2:5277/1‑128 (MQ=255)                                                                                                                                   aTTAGGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAGAACAACACTTtacta  <  2:911305/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCG‑GGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCGATGGCGTGACAGAACAACACTTTACTA  >  NC_000913/468758‑469027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑