Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,135	A→G	T6A (ACT→GCT)	htpG →	chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,135	0	A	G	100.0%	203.5 / NA	55	T6A (ACT→GCT)	htpG	chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/26);  total (29/26)

ATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCG  >  NC_000913/494999‑495269                                                                                                                                         |                                                                                                                                       aTTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTc                                                                                                                                     >  2:970882/1‑140 (MQ=255)     gTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTgg                                                                                                                                 >  1:1171059/1‑140 (MQ=255)     gTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTgg                                                                                                                                 >  2:70538/1‑140 (MQ=255)                      agCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGa                                                                                                                >  2:299760/1‑140 (MQ=255)                           gATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGaa                                                                                                               >  2:955186/1‑136 (MQ=255)                           gATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGaa                                                                                                               <  1:955186/136‑1 (MQ=255)                                ccTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCt                                                                                                      >  2:544995/1‑140 (MQ=255)                                 cTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTg                                                                                                     <  1:299760/140‑1 (MQ=255)                                 cTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTg                                                                                                     <  1:723063/140‑1 (MQ=255)                                    ctctCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTg                                                                                                     <  2:132446/137‑1 (MQ=255)                                    ctctCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTg                                                                                                     >  1:132446/1‑137 (MQ=255)                                         gCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCtgat                                                                                             >  2:1118407/1‑140 (MQ=255)                                         gCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCtgat                                                                                             <  1:70538/140‑1 (MQ=255)                                         gCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCtgat                                                                                             <  2:220842/140‑1 (MQ=255)                                           ttGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCtgatga                                                                                           <  2:521893/140‑1 (MQ=255)                                            tGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTgatgat                                                                                          <  1:544995/140‑1 (MQ=255)                                                attattCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCAt                                                                                      <  2:239903/140‑1 (MQ=255)                                                attattCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCAt                                                                                      <  1:609288/140‑1 (MQ=255)                                                 ttattCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCAtt                                                                                     <  1:740215/140‑1 (MQ=255)                                                   attCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATtct                                                                                   <  1:1118407/140‑1 (MQ=255)                                                         ccTTGTCCCCATCTCTCCCACGTCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCa                                                                                       <  1:102307/130‑1 (MQ=255)                                                         ccTTGTCCCCATCTCTCCCACGTCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCa                                                                                       >  2:102307/1‑130 (MQ=255)                                                             gTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCa                                                                         <  1:83382/140‑1 (MQ=255)                                                             gTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCa                                                                         <  2:433247/140‑1 (MQ=255)                                                                   aTCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCGTTCTCTCTATTCCAATAAAg                                                                   >  2:996538/1‑140 (MQ=255)                                                                   aTCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAg                                                                   >  1:552259/1‑140 (MQ=255)                                                                   aTCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAg                                                                   >  2:644444/1‑140 (MQ=255)                                                                         cccACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCt                                                             >  1:66381/1‑140 (MQ=255)                                                                             caTCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCt                                                         >  2:44970/1‑140 (MQ=255)                                                                               tCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGc                                                       <  2:332320/140‑1 (MQ=255)                                                                                 cTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCGTTCTCTCTATTCCAATAAAGAAATCTTCCTGCGt                                                     <  1:996538/140‑1 (MQ=255)                                                                                  tGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTg                                                    >  1:302424/1‑140 (MQ=255)                                                                                  tGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTg                                                    >  2:710115/1‑140 (MQ=255)                                                                                    tttttAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAg                                                  <  2:302424/140‑1 (MQ=255)                                                                                       ttAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCtt                                               <  1:1041485/140‑1 (MQ=255)                                                                                             ttAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                         <  1:710115/140‑1 (MQ=255)                                                                                                aaaTGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAAc                                      <  2:559225/140‑1 (MQ=255)                                                                                                           ttGAGGTAGACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgc                           <  1:644444/140‑1 (MQ=255)                                                                                                                   gACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACa                   >  2:227244/1‑140 (MQ=255)                                                                                                                   gACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACa                   <  1:227244/140‑1 (MQ=255)                                                                                                                   gACCTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACa                   >  1:877147/1‑140 (MQ=255)                                                                                                                   gACCTACATGAAAGGACAAGAAGCTCGCGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACa                   <  1:1100360/140‑1 (MQ=255)                                                                                                                     ccTACATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAg                 >  1:412797/1‑140 (MQ=255)                                                                                                                         cATGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGc             >  2:624130/1‑140 (MQ=255)                                                                                                                          aTGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCg            >  1:440965/1‑140 (MQ=255)                                                                                                                          aTGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCg            >  2:570818/1‑140 (MQ=255)                                                                                                                           tGAAAGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGt           >  2:1154743/1‑140 (MQ=255)                                                                                                                              aaGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTc        >  1:703992/1‑140 (MQ=255)                                                                                                                              aaGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTc        >  1:967210/1‑140 (MQ=255)                                                                                                                               aGGACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTcc       >  2:755589/1‑140 (MQ=255)                                                                                                                                 gACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCg            <  2:805889/133‑1 (MQ=255)                                                                                                                                 gACAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCg            >  1:805889/1‑133 (MQ=255)                                                                                                                                   cAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgc   >  2:622777/1‑140 (MQ=255)                                                                                                                                   cAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgc   <  2:66381/140‑1 (MQ=255)                                                                                                                                   cAAGAAGCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgc   >  2:1011712/1‑140 (MQ=255)                                                                                                                                    aagaagCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgcg  <  2:552259/140‑1 (MQ=255)                                                                                                                                    aagaagCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgcg  <  1:767120/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       ATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCG  >  NC_000913/494999‑495269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑