Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,344	C→T	A97V (GCG→GTG)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,344	0	C	T	100.0%	261.0 / NA	73	A97V (GCG→GTG)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/42);  total (31/42)

CGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGG  >  NC_000913/498207‑498475                                                                                                                                          |                                                                                                                                    cgTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAgtgc                                                                                                                                   >  2:951319/1‑140 (MQ=255) cgTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAgtgc                                                                                                                                   >  2:1002934/1‑140 (MQ=255) cgTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAgtgc                                                                                                                                   >  1:856961/1‑140 (MQ=255)  gTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCt                                                                                                                                  <  2:596290/140‑1 (MQ=255)  gTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCt                                                                                                                                  <  1:1128998/140‑1 (MQ=255)       tttGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGaa                                                                                                                             <  1:722229/140‑1 (MQ=255)           ccGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAg                                                                                                                         >  2:379099/1‑140 (MQ=255)             gCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCt                                                                                                                       >  2:1184946/1‑140 (MQ=255)                  gcTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGc                                                                                                                  <  2:609400/140‑1 (MQ=255)                      gccgcGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGc                                                                                                              <  1:1002934/140‑1 (MQ=255)                      gccgcGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGc                                                                                                              <  1:379099/140‑1 (MQ=255)                        cgcgTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCa                                                                                                            <  1:287848/140‑1 (MQ=255)                           gtgtGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCa                                                                                                         <  2:890858/140‑1 (MQ=255)                                        gTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCg                                                                                            <  1:951319/140‑1 (MQ=255)                                        gTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCg                                                                                            >  2:878551/1‑140 (MQ=255)                                             ccTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGAt                                                                                       <  1:15724/140‑1 (MQ=255)                                             ccTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGAt                                                                                       <  1:752757/140‑1 (MQ=255)                                                ctgtctgGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGaa                                                                                    >  1:247437/1‑140 (MQ=255)                                                ctgtctgGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGaa                                                                                    >  2:193117/1‑140 (MQ=255)                                                  gtctgGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACt                                                                                  >  1:880566/1‑140 (MQ=255)                                                   tctgGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAg                                                                                                 >  2:324665/1‑124 (MQ=255)                                                   tctgGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAg                                                                                                 <  1:324665/124‑1 (MQ=255)                                                   tctgGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTc                                                                                 >  2:1117762/1‑140 (MQ=255)                                                       gatggaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTgg                                                                             <  1:193117/140‑1 (MQ=255)                                                        atggaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGc                                                                            >  1:1195042/1‑140 (MQ=255)                                                        atggaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGc                                                                            <  2:283649/140‑1 (MQ=255)                                                        atggaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGc                                                                            >  1:773883/1‑140 (MQ=255)                                                         tggaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCa                                                                           <  1:213203/140‑1 (MQ=255)                                                         tggaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCa                                                                           <  2:856961/140‑1 (MQ=255)                                                          ggaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCag                                                                          <  1:878551/140‑1 (MQ=255)                                                          ggaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCag                                                                          <  1:329166/140‑1 (MQ=255)                                                           gaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCaga                                                                         <  1:140786/140‑1 (MQ=255)                                                           gaAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCaga                                                                         <  2:773883/140‑1 (MQ=255)                                                             aGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGc                                                                            <  1:657344/135‑1 (MQ=255)                                                             aGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGc                                                                            >  2:657344/1‑135 (MQ=255)                                                             aGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCag                                                                          <  2:709052/137‑1 (MQ=255)                                                             aGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGc                                                                       >  2:911410/1‑140 (MQ=255)                                                             aGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCATAGATGAACTCCTGGCag                                                                          >  1:709052/1‑137 (MQ=255)                                                               gTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCAt                                                                     <  1:609317/140‑1 (MQ=255)                                                                 ggCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGt                                                                   <  2:1195042/140‑1 (MQ=255)                                                                 ggCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGt                                                                   >  1:430788/1‑140 (MQ=255)                                                                 ggCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGt                                                                   <  1:1184946/140‑1 (MQ=255)                                                                  gCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTa                                                                  >  2:920908/1‑140 (MQ=255)                                                                  gCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTa                                                                  >  1:356132/1‑140 (MQ=255)                                                                  gCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTa                                                                  >  1:1089389/1‑140 (MQ=255)                                                                    tCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGa                                                                <  1:1117762/140‑1 (MQ=255)                                                                    tCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGa                                                                <  1:318972/140‑1 (MQ=255)                                                                      gccgcTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATc                                                              >  2:736996/1‑140 (MQ=255)                                                                      gccgcTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATc                                                              <  2:247437/140‑1 (MQ=255)                                                                       ccgcTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCa                                                             <  1:8366/140‑1 (MQ=255)                                                                          cTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCa                                                                           <  2:516334/123‑1 (MQ=255)                                                                          cTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCa                                                                           >  1:516334/1‑123 (MQ=255)                                                                            gATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATtg                                                        <  2:430788/140‑1 (MQ=255)                                                                              tGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATtgtg                                                      <  2:356132/140‑1 (MQ=255)                                                                                gTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTgtggt                                                    <  1:125769/140‑1 (MQ=255)                                                                                       gccgccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCg                                             >  1:1181200/1‑140 (MQ=255)                                                                                        ccgccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGc                                            >  1:101320/1‑140 (MQ=255)                                                                                          gccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCtt                                          >  2:511456/1‑140 (MQ=255)                                                                                           ccAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCttt                                         <  2:1181200/140‑1 (MQ=255)                                                                                                aaCAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCg                                    <  2:1089389/140‑1 (MQ=255)                                                                                                  cagcagGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCa                                  >  1:665743/1‑140 (MQ=255)                                                                                                  cagcagGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCa                                  >  1:723842/1‑140 (MQ=255)                                                                                                   agcagGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCaa                                 <  2:101320/140‑1 (MQ=255)                                                                                                        gcgcTGGCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATtctc                            <  1:911410/140‑1 (MQ=255)                                                                                                             ggCACAACGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGtt                       >  2:662536/1‑140 (MQ=255)                                                                                                                    cGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAcggt                <  2:723842/140‑1 (MQ=255)                                                                                                                    cGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAcggt                <  1:920908/140‑1 (MQ=255)                                                                                                                    cGTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAcggt                <  2:880566/140‑1 (MQ=255)                                                                                                                     gTTTACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAcggtc               <  1:736996/140‑1 (MQ=255)                                                                                                                        tACCGGAGATGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAcggtcggt            <  2:412191/140‑1 (MQ=255)                                                                                                                                aTGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATg    >  2:695760/1‑140 (MQ=255)                                                                                                                                aTGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATg    >  2:148301/1‑140 (MQ=255)                                                                                                                                aTGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATg    >  1:23989/1‑140 (MQ=255)                                                                                                                                 tGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATgg   >  1:1057422/1‑140 (MQ=255)                                                                                                                                 tGCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATgg   >  2:857110/1‑140 (MQ=255)                                                                                                                                  gCCCGTAGTGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATggg  >  2:554773/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    CGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTACGGTCGGTGCGGTATGGG  >  NC_000913/498207‑498475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑