Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,363	G→A	intergenic (‑230/+150)	metT ← / ← asnB	tRNA‑Met/asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,363	0	G	A	100.0%	207.2 / NA	57	intergenic (‑230/+150)	metT/asnB	tRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/35);  total (22/35)

GAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCG  >  NC_000913/697227‑697495                                                                                                                                         |                                                                                                                                     gAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGaaaa                                                                                                                                   <  1:1053839/140‑1 (MQ=255)      tGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAAt                                                                                                                              <  1:709099/140‑1 (MQ=255)      tGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAAt                                                                                                                              <  2:781764/140‑1 (MQ=255)             ttGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATaaa                                                                                                                       >  1:856787/1‑140 (MQ=255)                gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATa                                                                                                                    <  2:891808/140‑1 (MQ=255)                gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATa                                                                                                                    <  2:369124/140‑1 (MQ=255)                gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATa                                                                                                                    <  2:376897/140‑1 (MQ=255)                gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATa                                                                                                                    <  2:132526/140‑1 (MQ=255)                gTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATAAAATa                                                                                                                    <  2:856787/140‑1 (MQ=255)                     gTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAACATTAATCTATGAAATACTGGc                                                                                                               <  2:875207/140‑1 (MQ=255)                      ttGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGcc                                                                                                              >  2:284278/1‑140 (MQ=255)                       tGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCt                                                                                                             <  1:667200/140‑1 (MQ=255)                        gCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTa                                                                                                            <  2:210848/140‑1 (MQ=255)                        gCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTa                                                                                                            >  1:438846/1‑140 (MQ=255)                        gCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTa                                                                                                            <  1:1138232/140‑1 (MQ=255)                        gCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTa                                                                                                            <  1:989982/140‑1 (MQ=255)                          gAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACa                                                                                                          <  2:661553/140‑1 (MQ=255)                           aaCAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAg                                                                                                         <  1:923857/140‑1 (MQ=255)                               gCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGtgat                                                                                                     >  1:463056/1‑140 (MQ=255)                                   ggCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGt                                                                                                 <  1:961321/140‑1 (MQ=255)                                   ggCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGt                                                                                                 >  1:909345/1‑140 (MQ=255)                                     cgcTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTg                                                                                               <  1:284278/140‑1 (MQ=255)                                        tataTTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCa                                                                                            <  2:150589/140‑1 (MQ=255)                                          tatTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCaaa                                                                                          <  1:56418/140‑1 (MQ=255)                                                tCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGa                                                                                    >  1:848915/1‑140 (MQ=255)                                                 cAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGAt                                                                                   <  2:1096688/140‑1 (MQ=255)                                                  aaTTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAg                                                                                                  >  1:47350/1‑124 (MQ=255)                                                  aaTTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAg                                                                                                  <  2:47350/124‑1 (MQ=255)                                                    ttattGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCa                                                                                            >  2:1205616/1‑128 (MQ=255)                                                    ttattGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCa                                                                                            <  1:1205616/128‑1 (MQ=255)                                                       ttGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCa                                                                             <  1:156952/140‑1 (MQ=255)                                                              cTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCgg                                                                      >  1:1048345/1‑140 (MQ=255)                                                                 tgtgTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAAc                                                                   >  2:210684/1‑140 (MQ=255)                                                                       ttttCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTcc                                                             <  2:1048345/140‑1 (MQ=255)                                                                             cACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTa                                                          >  1:496810/1‑137 (MQ=255)                                                                             cACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTa                                                          <  2:496810/137‑1 (MQ=255)                                                                               cccTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCa                                                     >  2:875662/1‑140 (MQ=255)                                                                                  tACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCatat                                                  >  1:132533/1‑140 (MQ=255)                                                                                     aGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAg                                               <  2:132533/140‑1 (MQ=255)                                                                                     aGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAg                                               <  2:473063/140‑1 (MQ=255)                                                                                          ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTGCAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAAt                                            >  2:1073629/1‑138 (MQ=255)                                                                                          ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAAt                                            <  1:1073629/138‑1 (MQ=255)                                                                                          ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATca                                          >  2:234961/1‑140 (MQ=255)                                                                                          ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATca                                          >  2:299614/1‑140 (MQ=255)                                                                                          ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATca                                          >  2:314465/1‑140 (MQ=255)                                                                                          ttCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATca                                          >  2:1207859/1‑140 (MQ=255)                                                                                           tCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATcaa                                         >  2:169069/1‑140 (MQ=255)                                                                                           tCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATcaa                                         >  2:710044/1‑140 (MQ=255)                                                                                            cTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATcaac                                        <  2:188043/140‑1 (MQ=255)                                                                                             ttttGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATcaaca                                       <  1:314465/140‑1 (MQ=255)                                                                                               ttGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAg                                     <  1:1207859/140‑1 (MQ=255)                                                                                                tGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGc                                    <  1:875662/140‑1 (MQ=255)                                                                                                      aCAGGATGAAATTCGGAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCa                              <  1:299614/140‑1 (MQ=255)                                                                                                                    ggAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCa                              >  2:212892/1‑126 (MQ=255)                                                                                                                    ggAATTTAACAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCa                              <  1:212892/126‑1 (MQ=255)                                                                                                                           aaCAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGAt         <  2:909345/140‑1 (MQ=255)                                                                                                                             cAATAGTGGTGGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGAttt       <  1:169069/140‑1 (MQ=255)                                                                                                                                  gtggtgGAAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCg  >  2:104580/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     GAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCG  >  NC_000913/697227‑697495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑