Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	910,772	A→G	V93A (GTT→GCT)	poxB ←	pyruvate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	910,772	0	A	G	100.0%	173.8 / NA	48	V93A (GTT→GCT)	poxB	pyruvate oxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/20);  total (28/20)

ATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGC  >  NC_000913/910640‑910907                                                                                                                                     |                                                                                                                                        aTCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGAtt                                                                                                                                  >  1:1230110/1‑140 (MQ=255) aTCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGAtt                                                                                                                                  >  1:255254/1‑140 (MQ=255)    tGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCg                                                                                                                               >  1:960965/1‑140 (MQ=255)     gCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCgg                                                                                                                              >  2:238538/1‑140 (MQ=255)     gCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCgg                                                                                                                              <  1:3438/140‑1 (MQ=255)       tCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTg                                                                                                                            >  1:1089689/1‑140 (MQ=255)                gAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGaa                                                                                                                   >  2:1083140/1‑140 (MQ=255)                gAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGaa                                                                                                                   >  1:588979/1‑140 (MQ=255)                 aaaCCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAAc                                                                                                                  <  2:1230110/140‑1 (MQ=255)                     cAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGc                                                                                                              <  2:62191/140‑1 (MQ=255)                      aGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGcc                                                                                                             <  1:136619/140‑1 (MQ=255)                                  tGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTg                                                                                                 >  2:42487/1‑140 (MQ=255)                                     cTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAg                                                                                              <  1:42487/140‑1 (MQ=255)                                     cTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAg                                                                                              >  2:251995/1‑140 (MQ=255)                                            cgcgGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCg                                                                                       >  2:1055037/1‑140 (MQ=255)                                                    aGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCa                                                                               <  1:1055037/140‑1 (MQ=255)                                                        ctTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGAt                                                                           <  1:238538/140‑1 (MQ=255)                                                           gtgggtggGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCg                                                                        >  1:2166/1‑140 (MQ=255)                                                           gtgggtggGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCg                                                                        >  2:1151781/1‑140 (MQ=255)                                                           gtgggtggGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCg                                                                        >  1:664945/1‑140 (MQ=255)                                                              ggtgggtTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGgcg                                                                     >  2:961708/1‑140 (MQ=255)                                                                   gtTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGAc                                                                >  1:848123/1‑140 (MQ=255)                                                                      tCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGc                                                             <  2:741339/140‑1 (MQ=255)                                                                        cTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCa                                                           >  2:915468/1‑140 (MQ=255)                                                                          ggAAATAGCCGCTGCCAATTTCGCTGGAGGGACTATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCATGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGt                                                         <  2:1089689/140‑1 (MQ=255)                                                                           gAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGtt                                                        <  2:588979/140‑1 (MQ=255)                                                                              aTAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGtt                                                        <  1:864004/137‑1 (MQ=255)                                                                              aTAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGtt                                                        >  2:864004/1‑137 (MQ=255)                                                                                aGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCg                                                   <  2:848123/140‑1 (MQ=255)                                                                                aGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCg                                                   <  1:961708/140‑1 (MQ=255)                                                                                 gCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGc                                                  >  1:345856/1‑140 (MQ=255)                                                                                  ccGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCt                                                 <  1:1083140/140‑1 (MQ=255)                                                                                  ccGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCt                                                 <  2:887506/140‑1 (MQ=255)                                                                                            tttCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCt                                       <  1:116012/140‑1 (MQ=255)                                                                                            tttCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCt                                       >  2:139814/1‑140 (MQ=255)                                                                                                    gAGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCGGCGcc                               <  1:1151781/140‑1 (MQ=255)                                                                                                     aGGGAATATGAGCGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCa                              <  2:255254/140‑1 (MQ=255)                                                                                                                 cGGCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgcc                  >  1:1111474/1‑140 (MQ=255)                                                                                                                   gCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAg                     >  2:645351/1‑135 (MQ=255)                                                                                                                   gCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAg                     <  1:645351/135‑1 (MQ=255)                                                                                                                   gCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccgc                >  1:191909/1‑140 (MQ=255)                                                                                                                   gCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccgc                >  2:819430/1‑140 (MQ=255)                                                                                                                   gCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccgc                >  1:28175/1‑140 (MQ=255)                                                                                                                   gCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccgc                >  2:402810/1‑140 (MQ=255)                                                                                                                   gCAATCGCCAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCCCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccgc                >  2:856706/1‑140 (MQ=255)                                                                                                                           cAGTACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATACGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCActtctt        >  2:552689/1‑140 (MQ=255)                                                                                                                              tACCGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGt     >  1:178041/1‑140 (MQ=255)                                                                                                                                 cGGAGCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGc  <  1:767863/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        ATCTGCTCCGGGCTGGAAACCAGCTCGCAATAGTGACTACATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGC  >  NC_000913/910640‑910907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑