Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	975,341	A→G	E341E (GAA→GAG)	mukF →	chromosome partitioning protein MukF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	975,341	0	A	G	100.0%	246.2 / NA	66	E341E (GAA→GAG)	mukF	chromosome partitioning protein MukF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/34);  total (32/34)

TGCTCAGCGGTTACGTCAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAACTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCTGGTGGTACGCGAATATCTGT  >  NC_000913/975209‑975477                                                                                                                                     |                                                                                                                                         tGCTCAGCGGTTACGTCAGTCGGTACAATCCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTg                                                                                                                                   <  1:734511/140‑1 (MQ=255) tGCTCAGCGGTTACGTCAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTg                                                                                                                                   <  1:1032749/140‑1 (MQ=255) tGCTCAGCGGTTACGTCAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTg                                                                                                                                   <  1:401816/140‑1 (MQ=255)    tCAGCGGTTACGTCAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAgg                                                                                                                                <  1:972499/140‑1 (MQ=255)        cGGTTACGTCAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCt                                                                                                                            >  2:524936/1‑140 (MQ=255)        cGGTTACGTCAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCt                                                                                                                            >  2:478884/1‑140 (MQ=255)           ttACGTCAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGa                                                                                                                         >  1:1214999/1‑140 (MQ=255)                 cAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGa                                                                                                                   <  2:104431/140‑1 (MQ=255)                 cAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGa                                                                                                                   <  2:929905/140‑1 (MQ=255)                  aGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGaa                                                                                                                  >  2:38882/1‑140 (MQ=255)                      ggTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGt                                                                                                              <  2:199372/140‑1 (MQ=255)                                  ttttGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATcc                                                                                                  >  2:648671/1‑140 (MQ=255)                                    ttGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCcgc                                                                                                >  1:533548/1‑140 (MQ=255)                                       atgaGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGt                                                                                                              >  2:955397/1‑123 (MQ=255)                                       atgaGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGt                                                                                                              <  1:955397/123‑1 (MQ=255)                                         gaGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACga                                                                                                       <  2:7800/128‑1 (MQ=255)                                         gaGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACga                                                                                                       >  1:7800/1‑128 (MQ=255)                                         gaGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACa                                                                                           >  1:984889/1‑140 (MQ=255)                                            ccGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCt                                                                                        >  2:713838/1‑140 (MQ=255)                                                  gcgcTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTggcggc                                                                                  <  1:142454/140‑1 (MQ=255)                                                     cTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGat                                                                               >  2:1183968/1‑140 (MQ=255)                                                      tAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAg                                                                                          >  2:159003/1‑128 (MQ=255)                                                      tAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAg                                                                                          <  1:159003/128‑1 (MQ=255)                                                      tAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGatc                                                                              >  1:683617/1‑140 (MQ=255)                                                      tAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGatc                                                                              >  1:173926/1‑140 (MQ=255)                                                      tAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGatc                                                                              >  2:1039986/1‑140 (MQ=255)                                                      tAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGatc                                                                              >  1:988232/1‑140 (MQ=255)                                                           tATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCga                                                                         >  2:1008715/1‑140 (MQ=255)                                                                  aTGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGTGGCGATCATCGAAGaacaa                                                                  >  1:277838/1‑140 (MQ=255)                                                                  aTGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGatcat                                                                            <  1:703820/130‑1 (MQ=255)                                                                  aTGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGatcat                                                                            >  2:703820/1‑130 (MQ=255)                                                                  aTGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGat                                                                               >  1:181866/1‑127 (MQ=255)                                                                  aTGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGat                                                                               <  2:181866/127‑1 (MQ=255)                                                                  aTGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGaacaa                                                                  >  2:746640/1‑140 (MQ=255)                                                                  aTGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGaacaa                                                                  >  2:286595/1‑140 (MQ=255)                                                                   tGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGaacaac                                                                 <  1:943471/140‑1 (MQ=255)                                                                    gCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACt                                                                <  1:38882/140‑1 (MQ=255)                                                                      cGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTg                                                              <  1:478884/140‑1 (MQ=255)                                                                      cGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTg                                                              <  2:1214999/140‑1 (MQ=255)                                                                      cGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGAGATGAAGAAGTGACTGGGGAGCTTCCTGAGGACCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTg                                                              <  2:1147277/140‑1 (MQ=255)                                                                        aTCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGTGGCGATCATCGAAGAACAACTTGcc                                                            <  2:277838/140‑1 (MQ=255)                                                                        aTCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGcc                                                            >  2:662663/1‑140 (MQ=255)                                                                        aTCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGcc                                                            <  1:524936/140‑1 (MQ=255)                                                                        aTCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGcc                                                            <  1:1183968/140‑1 (MQ=255)                                                                        aTCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACAGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGcc                                                            <  1:1074133/140‑1 (MQ=255)                                                                                ctgGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACaa                                                    <  1:662663/140‑1 (MQ=255)                                                                                  ggATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACaaaa                                                  <  1:746640/140‑1 (MQ=255)                                                                                        gCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTAGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGAc                                            >  2:243222/1‑140 (MQ=255)                                                                                             aCGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTg                                       >  1:289717/1‑140 (MQ=255)                                                                                                aaGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCg                                    <  1:713838/140‑1 (MQ=255)                                                                                                  gagaTGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCt                                  <  1:648671/140‑1 (MQ=255)                                                                                                      tGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGAt                              <  2:208656/140‑1 (MQ=255)                                                                                                      tGGCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGAt                              <  2:173926/140‑1 (MQ=255)                                                                                                       ggCACTGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATc                             <  2:702425/140‑1 (MQ=255)                                                                                                            tGCGCGATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGt                        >  1:422524/1‑140 (MQ=255)                                                                                                              cgcgATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGa                               >  1:759768/1‑131 (MQ=255)                                                                                                              cgcgATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGa                               <  2:759768/131‑1 (MQ=255)                                                                                                              cgcgATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCt                      >  1:735840/1‑140 (MQ=255)                                                                                                              cgcgATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCt                      >  2:383424/1‑140 (MQ=255)                                                                                                              cgcgATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCt                      >  2:352642/1‑140 (MQ=255)                                                                                                              cgcgATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCt                      >  2:945820/1‑140 (MQ=255)                                                                                                                 gATGAAGAAGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCtggt                   >  1:349345/1‑140 (MQ=255)                                                                                                                    gaagaaGTGACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCTggtggt                <  2:988232/140‑1 (MQ=255)                                                                                                                            gACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCTGGTGGTACGCGAat        <  2:683617/140‑1 (MQ=255)                                                                                                                            gACTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCTGGTGGTACGCGAat        <  1:945820/140‑1 (MQ=255)                                                                                                                             aCTGGGGAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCTGGTGGTACGCGAata       >  1:211054/1‑140 (MQ=255)                                                                                                                                  ggAGCTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCTGGTGGTACGCGAATATCTGt  <  2:984889/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TGCTCAGCGGTTACGTCAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCCGATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGGGAACTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCGATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCTGGTGGTACGCGAATATCTGT  >  NC_000913/975209‑975477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑