Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,014,720	A→G	M488V (ATG→GTG)	pqiB →	intermembrane transport protein PqiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,014,720	0	A	G	100.0%	249.6 / NA	68	M488V (ATG→GTG)	pqiB	intermembrane transport protein PqiB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/31);  total (37/31)

CGCTTTCTGAAAGTCAGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCATGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGAGAAGAGTAACGCGCTGG  >  NC_000913/1014586‑1014846                                                                                                                                       |                                                                                                                               cGCTTTCTGAAAGTCAGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACGAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAg                                                                                                                           >  2:852337/1‑140 (MQ=255) cGCTTTCTGAAAGTCAGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAg                                                                                                                           >  2:738809/1‑140 (MQ=255)      tCTGAAAGTCAGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCtt                                                                                                                      >  2:440855/1‑140 (MQ=255)      tCTGAAAGTCAGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCtt                                                                                                                      >  1:213526/1‑140 (MQ=255)       cTGAAAGTCAGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTc                                                                                                                     <  1:1096353/140‑1 (MQ=255)              tCAGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAg                                                                                                                           <  1:583462/127‑1 (MQ=255)              tCAGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAg                                                                                                                           >  2:583462/1‑127 (MQ=255)                aGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGc                                                                                                            >  1:975031/1‑140 (MQ=255)                aGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGc                                                                                                            >  2:772532/1‑140 (MQ=255)                aGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGc                                                                                                            >  2:500466/1‑140 (MQ=255)                    cacaATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCg                                                                                                        <  2:213526/140‑1 (MQ=255)                        aTGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGc                                                                                                    >  2:816305/1‑140 (MQ=255)                         tGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGcc                                                                                                   <  2:447172/140‑1 (MQ=255)                           aaaaaCCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTa                                                                                                 <  1:440855/140‑1 (MQ=255)                           aaaaaCCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTa                                                                                                 <  2:1218056/140‑1 (MQ=255)                                 cTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCg                                                                                                        >  2:622060/1‑127 (MQ=255)                                 cTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCg                                                                                                        <  1:622060/127‑1 (MQ=255)                                   gCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGa                                                                                         >  1:120182/1‑140 (MQ=255)                                   gCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGa                                                                                         >  2:915663/1‑140 (MQ=255)                                       acgacgCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGAtggt                                                                                     <  1:772532/140‑1 (MQ=255)                                         gacgCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGAtggtgg                                                                                   <  1:852337/140‑1 (MQ=255)                                          acgCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAg                                                                                          <  1:135455/132‑1 (MQ=255)                                          acgCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAg                                                                                          >  2:135455/1‑132 (MQ=255)                                             cTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGa                                                                               <  1:580090/140‑1 (MQ=255)                                              tGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGat                                                                              <  2:62838/140‑1 (MQ=255)                                                   aGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCa                                                                         <  1:1222189/140‑1 (MQ=255)                                                        gAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGcc                                                                    <  1:500466/140‑1 (MQ=255)                                                        gAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGcc                                                                    <  2:854740/140‑1 (MQ=255)                                                          aCAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCa                                                                         <  1:596963/133‑1 (MQ=255)                                                          aCAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCa                                                                         >  2:596963/1‑133 (MQ=255)                                                          aCAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCtt                                                                  >  1:88893/1‑140 (MQ=255)                                                           cAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTg                                                                 <  1:915663/140‑1 (MQ=255)                                                               aTCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCa                                                             >  2:937151/1‑140 (MQ=255)                                                               aTCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCa                                                             >  2:209772/1‑140 (MQ=255)                                                                tCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAg                                                            <  1:151513/140‑1 (MQ=255)                                                                   tCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGgtg                                                         >  1:472882/1‑140 (MQ=255)                                                                   tCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGgtg                                                         >  1:673237/1‑140 (MQ=255)                                                                   tCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGgtg                                                         >  1:677542/1‑140 (MQ=255)                                                                      cTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAgcg                                                                      <  1:678422/124‑1 (MQ=255)                                                                      cTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAgcg                                                                      >  2:678422/1‑124 (MQ=255)                                                                        aGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCg                                                    >  1:212762/1‑140 (MQ=255)                                                                         gCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCga                                                   <  2:120182/140‑1 (MQ=255)                                                                          ccAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCgag                                                  >  2:366768/1‑140 (MQ=255)                                                                           cAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCgaga                                                 <  1:209772/140‑1 (MQ=255)                                                                              tCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCga                                                   <  1:656039/135‑1 (MQ=255)                                                                              tCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCga                                                   >  2:656039/1‑135 (MQ=255)                                                                              tCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACt                                              >  1:149536/1‑140 (MQ=255)                                                                                   gcagcaGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAAc                                         >  2:525113/1‑140 (MQ=255)                                                                                        agTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTg                                    >  2:728885/1‑140 (MQ=255)                                                                                            gCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGa                                >  2:81835/1‑140 (MQ=255)                                                                                             ccGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGaa                               <  1:525113/140‑1 (MQ=255)                                                                                             ccGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGaa                               <  2:677542/140‑1 (MQ=255)                                                                                               gACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGaaaa                             >  1:45229/1‑140 (MQ=255)                                                                                                 cGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACg                           <  1:816305/140‑1 (MQ=255)                                                                                                  ggATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGc                          <  2:591508/140‑1 (MQ=255)                                                                                                       tGCAGTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAAt                     <  2:472882/140‑1 (MQ=255)                                                                                                           gTCAACGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATgaga                 >  2:110875/1‑140 (MQ=255)                                                                                                              aaCGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGagaaga              >  1:613221/1‑140 (MQ=255)                                                                                                              aaCGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGagaa                >  2:585178/1‑138 (MQ=255)                                                                                                              aaCGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGagaa                <  1:585178/138‑1 (MQ=255)                                                                                                                cGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAAt                     >  2:1169371/1‑131 (MQ=255)                                                                                                                cGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAAt                     <  1:1169371/131‑1 (MQ=255)                                                                                                                cGTTGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGAGAAGAGt            >  2:391905/1‑140 (MQ=255)                                                                                                                  ttGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGAGAAGAGTaa          <  2:128151/140‑1 (MQ=255)                                                                                                                   tGCGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGAGAAGAGTAAc         >  1:27795/1‑140 (MQ=255)                                                                                                                     cGTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGAGAAGAGTAAcgc       >  2:168008/1‑140 (MQ=255)                                                                                                                      gTGAATTGAATCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGAGAAGAGTAAcgcg      <  2:212762/140‑1 (MQ=255)                                                                                                                          attgaatCGCAGCGTGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGAGAAGAGTAACGCGCTgg  >  1:166738/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                               CGCTTTCTGAAAGTCAGCGCACAATGAAAAACCTGCAAACGACGCTGGATAGCATGAACAAGATCCTCGCTAGCCAGTCGATGCAGCAGTTGCCGACGGATATGCAGTCAACGTTGCGTGAATTGAATCGCAGCATGCAGGGCTTCCAGCCTGGCTCCGCAGCCTACAACAAGATGGTGGCGGATATGCAGCGCCTTGATCAGGTGTTGCGAGAACTGCAACCGGTGCTGAAAACGCTCAATGAGAAGAGTAACGCGCTGG  >  NC_000913/1014586‑1014846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑