Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,127,049	A→G	T82A (ACG→GCG)	yceM →	putative oxidoreductase YceM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,127,049	0	A	G	100.0%	138.4 / NA	38	T82A (ACG→GCG)	yceM	putative oxidoreductase YceM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/11);  total (27/11)

GCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCA  >  NC_000913/1126913‑1127183                                                                                                                                         |                                                                                                                                       gCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGt                                                                                                                                     >  1:558181/1‑140 (MQ=255)  ccTACGCGCGCGAAAGCCCTGCCAATTTGGGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGtt                                                                                                                                    >  2:289645/1‑140 (MQ=255)   cTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTa                                                                                                                                   <  2:558181/140‑1 (MQ=255)     aCGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCATTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACt                                                                                                                                 <  1:800224/140‑1 (MQ=255)           gcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGc                                                                                                                           <  2:220360/140‑1 (MQ=255)                      gCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATg                                                                                                                <  2:486283/140‑1 (MQ=255)                          aTTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCtg                                                                                                            <  1:289645/140‑1 (MQ=255)                                        gcgcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGc                                                                                              >  1:1016743/1‑140 (MQ=255)                                             ttCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCa                                                                                         >  1:23167/1‑140 (MQ=255)                                                     gCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCt                                                                                 >  2:477927/1‑140 (MQ=255)                                                         aTTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgcgc                                                                             >  1:688147/1‑140 (MQ=255)                                                          ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGcgcg                                                                            >  1:329648/1‑140 (MQ=255)                                                          ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGcgcg                                                                            >  1:391808/1‑140 (MQ=255)                                                          ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGcgcg                                                                            >  2:610909/1‑140 (MQ=255)                                                          ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGcgcg                                                                            >  1:490741/1‑140 (MQ=255)                                                           tCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGa                                                                           <  1:388155/140‑1 (MQ=255)                                                                    aGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACg                                                                  >  1:1072131/1‑140 (MQ=255)                                                                      ccTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGc                                                                >  1:1142119/1‑140 (MQ=255)                                                                             gccAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGa                                                         <  1:477927/140‑1 (MQ=255)                                                                                            gTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGaa                                          >  2:1036837/1‑140 (MQ=255)                                                                                            gTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGaa                                          <  2:490741/140‑1 (MQ=255)                                                                                                  gtgCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACt                                    >  2:346202/1‑140 (MQ=255)                                                                                                         ccAGCACCGCCAGCCACTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTg                             <  1:1104673/140‑1 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGTAAAAACTGACGTTGATGGTCGGt                    >  2:1006442/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:565618/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:715295/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:463344/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:769045/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:102474/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  2:143160/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:858797/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:480242/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:460649/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:199189/1‑140 (MQ=255)                                                                                                                  ccagccaCTTTGACGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGAGCGGAAAAAACTGACGTTGATGGTCGGt                    >  1:68919/1‑140 (MQ=255)                                                                                                                               cGTGGTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGtt       >  1:109487/1‑140 (MQ=255)                                                                                                                                  ggTCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCg    <  2:1142119/140‑1 (MQ=255)                                                                                                                                    tCAGTGCGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGca  <  1:610909/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCA  >  NC_000913/1126913‑1127183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑