| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,134,936 | A→G | T70A (ACG→GCG) | flgG → | flagellar basal‑body rod protein FlgG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,134,936 | 0 | A | G | 96.7% | 207.2 / ‑2.9 | 61 | T70A (ACG→GCG) | flgG | flagellar basal‑body rod protein FlgG |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (28/31); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
ACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCAT > NC_000913/1134799‑1135062 | acaacCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGcgcg > 1:203446/1‑140 (MQ=255) caacCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCgg > 2:118312/1‑140 (MQ=255) tAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTg < 1:1073604/140‑1 (MQ=255) tttAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCtt > 2:976326/1‑140 (MQ=255) tttAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCtt > 2:896466/1‑140 (MQ=255) tttAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCtt > 2:799385/1‑140 (MQ=255) tttAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCtt > 2:422894/1‑140 (MQ=255) tAAGCGTCAGCGCGCGGCGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTac > 2:921961/1‑140 (MQ=255) aaGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTaca < 2:1215170/140‑1 (MQ=255) aaGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTaca < 1:118312/140‑1 (MQ=255) aaGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTaca < 1:752775/140‑1 (MQ=255) cGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCTGTCGCCACTGAACGCTTACACAg < 2:975553/140‑1 (MQ=255) cAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAg < 1:16221/137‑1 (MQ=255) cAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAg > 2:16221/1‑137 (MQ=255) cgcgGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGaa > 1:494018/1‑140 (MQ=255) ggTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAAcc < 1:921961/140‑1 (MQ=255) tgtTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTg > 1:636763/1‑140 (MQ=255) tGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGc < 1:592770/140‑1 (MQ=255) aGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGa < 2:617206/140‑1 (MQ=255) tCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAAcc > 2:280945/1‑128 (MQ=255) tCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAAcc < 1:280945/128‑1 (MQ=255) tCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGc > 2:221831/1‑134 (MQ=255) tCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGc < 1:221831/134‑1 (MQ=255) tCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACca > 2:497965/1‑140 (MQ=255) tCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACca > 2:892558/1‑140 (MQ=255) tCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACca > 1:1219201/1‑140 (MQ=255) tCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACca > 1:476739/1‑140 (MQ=255) tGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACcaac > 1:32380/1‑140 (MQ=255) tGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACcaac < 2:32380/140‑1 (MQ=255) tGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACcaac < 1:892558/140‑1 (MQ=255) tGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACcaac > 2:225883/1‑140 (MQ=255) gCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACcaaca < 2:636763/140‑1 (MQ=255) cTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACcaacaa > 2:797878/1‑140 (MQ=255) cTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACcaacaa < 1:799385/140‑1 (MQ=255) tCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCa > 2:1203529/1‑140 (MQ=255) ccATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGAt > 1:1218287/1‑140 (MQ=255) aTTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGt < 2:476739/140‑1 (MQ=255) ttCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTc > 1:1140545/1‑140 (MQ=255) ttCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTc > 1:655300/1‑140 (MQ=255) cGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTcgc < 2:297828/140‑1 (MQ=255) ccGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTaa > 1:1149625/1‑140 (MQ=255) aGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCGACAACAGCAAAGATGTCGCGATTAAagggcagggc > 2:529481/1‑140 (MQ=255) aaCAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAg > 1:639054/1‑140 (MQ=255) aCAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAgg > 2:308975/1‑140 (MQ=255) cAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGt < 1:968175/140‑1 (MQ=255) aaaCCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTg > 1:1023677/1‑140 (MQ=255) aaaCCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTg < 2:655300/140‑1 (MQ=255) aaaCCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTg < 1:976326/140‑1 (MQ=255) aaCCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGa < 2:1023677/140‑1 (MQ=255) ccTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTg < 1:896466/140‑1 (MQ=255) ccTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTg < 2:1218287/140‑1 (MQ=255) tACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCa < 2:1219201/140‑1 (MQ=255) cccTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGa < 1:802417/140‑1 (MQ=255) ccTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGAt > 2:143668/1‑140 (MQ=255) ccTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGAt < 1:1203529/140‑1 (MQ=255) tCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATgg > 2:1228362/1‑140 (MQ=255) cGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGtt > 1:190936/1‑140 (MQ=255) aTTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTtcat < 1:797878/140‑1 (MQ=255) aTTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTtcat < 2:36777/140‑1 (MQ=255) aTTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTtcat < 1:854414/140‑1 (MQ=255) aTTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTtcat < 1:497965/140‑1 (MQ=255) aTTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTtcat < 1:308975/140‑1 (MQ=255) aTTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTtcat < 1:225883/140‑1 (MQ=255) | ACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCAT > NC_000913/1134799‑1135062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |