Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,140,782	A→G	L250L (TTA→TTG)	flgL →	flagellar hook‑filament junction protein 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,140,782	0	A	G	100.0%	251.1 / NA	68	L250L (TTA→TTG)	flgL	flagellar hook‑filament junction protein 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/33);  total (35/33)

TGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCAGCAAACG  >  NC_000913/1140650‑1140917                                                                                                                                     |                                                                                                                                        tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGc                                                                                                                                  >  2:976227/1‑140 (MQ=255) tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGc                                                                                                                                  >  1:592710/1‑140 (MQ=255) tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGc                                                                                                                                  >  2:90005/1‑140 (MQ=255) tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGc                                                                                                                                  >  1:305110/1‑140 (MQ=255)   ccATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAg                                                                                                                                >  2:464515/1‑140 (MQ=255)   ccATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAg                                                                                                                                >  1:187543/1‑140 (MQ=255)      tCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTg                                                                                                                             >  2:1077860/1‑140 (MQ=255)      tCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTg                                                                                                                             >  1:229249/1‑140 (MQ=255)      tCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTg                                                                                                                             >  2:197707/1‑140 (MQ=255)           gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaacga                                                                                                                        >  2:433287/1‑140 (MQ=255)           gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaacga                                                                                                                        >  2:724063/1‑140 (MQ=255)           gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaacga                                                                                                                        >  1:399211/1‑140 (MQ=255)           gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaa                                                                                                                           <  2:829318/137‑1 (MQ=255)           gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaa                                                                                                                           >  1:829318/1‑137 (MQ=255)            cccTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaacgaa                                                                                                                       >  1:761185/1‑140 (MQ=255)              cTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACt                                                                                                                     >  2:435873/1‑140 (MQ=255)                gAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTgg                                                                                                                   <  1:976227/140‑1 (MQ=255)                  aaaCGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAg                                                                                                                 <  1:464515/140‑1 (MQ=255)                      gCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGc                                                                                                             >  2:959667/1‑140 (MQ=255)                      gCCGGTCCCGGATAGCGAAGCGGATAAAGAAACCGCCGCCGCCGCGCTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGCATTGGGCACGCAGCTGAACGAACTGGAGCCGc                                                                                                             >  2:742264/1‑140 (MQ=255)                        cGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTg                                                                                                           <  1:372536/140‑1 (MQ=255)                         ggTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTTGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                          <  1:1077860/140‑1 (MQ=255)                         ggTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                          <  2:187543/140‑1 (MQ=255)                         ggTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                          <  1:1111464/140‑1 (MQ=255)                         ggTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                          <  2:788910/140‑1 (MQ=255)                           tCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGAt                                                                                                        <  1:79498/140‑1 (MQ=255)                           tCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGAt                                                                                                        <  1:1092775/140‑1 (MQ=255)                           tCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGAt                                                                                                        <  1:197707/140‑1 (MQ=255)                              cgGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCa                                                                                                     <  2:553268/140‑1 (MQ=255)                                    gCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGt                                                                                               <  2:399211/140‑1 (MQ=255)                                          cGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGAt                                                                                         >  1:115378/1‑140 (MQ=255)                                            gATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATcg                                                                                       <  2:761185/140‑1 (MQ=255)                                             aTAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATcgc                                                                                      >  1:1017667/1‑140 (MQ=255)                                                    aaCCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAg                                                                               >  1:35658/1‑140 (MQ=255)                                                       cgccgcTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGc                                                                            >  1:659709/1‑140 (MQ=255)                                                               cggcgTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACgcag                                                                    <  2:605551/140‑1 (MQ=255)                                                                 gcgTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACgcagca                                                                  >  2:848687/1‑140 (MQ=255)                                                                    ttAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACgcag                                                                    <  1:443713/135‑1 (MQ=255)                                                                    ttAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACgcag                                                                    >  2:443713/1‑135 (MQ=255)                                                                    ttAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGAt                                                               <  1:175607/140‑1 (MQ=255)                                                                       gATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAg                                                            <  2:229249/140‑1 (MQ=255)                                                                       gATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAg                                                            <  1:532305/140‑1 (MQ=255)                                                                       gATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAg                                                            <  2:524116/140‑1 (MQ=255)                                                                          aaaaCCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGa                                                         <  1:435873/140‑1 (MQ=255)                                                                          aaaaCCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGa                                                         <  2:882079/140‑1 (MQ=255)                                                                          aaaaCCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGa                                                         <  1:959667/140‑1 (MQ=255)                                                                            aaccaaccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATc                                                       >  2:508450/1‑140 (MQ=255)                                                                               caaccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTgg                                                    <  2:659709/140‑1 (MQ=255)                                                                                aaccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGt                                                   <  2:1017667/140‑1 (MQ=255)                                                                                aaccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGt                                                   <  1:90005/140‑1 (MQ=255)                                                                                 accGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGtt                                                  >  2:444564/1‑140 (MQ=255)                                                                                 accGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATGCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGtt                                                  >  2:1074850/1‑140 (MQ=255)                                                                                    gcgGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGAt                                               <  1:848687/140‑1 (MQ=255)                                                                                         cTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGa                                          <  1:1074850/140‑1 (MQ=255)                                                                                            aaaaaCTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTg                                       >  1:878514/1‑140 (MQ=255)                                                                                             aaaaCTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTgg                                      >  2:484351/1‑140 (MQ=255)                                                                                               aaCTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGaa                                    <  2:35658/140‑1 (MQ=255)                                                                                                      tGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCaa                               <  2:769362/138‑1 (MQ=255)                                                                                                      tGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCaa                               >  1:769362/1‑138 (MQ=255)                                                                                                            aTGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCa                       >  1:543561/1‑140 (MQ=255)                                                                                                                   gACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAca                <  1:508450/140‑1 (MQ=255)                                                                                                                    aCTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAcat               >  2:783054/1‑140 (MQ=255)                                                                                                                    aCTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAcat               >  2:418159/1‑140 (MQ=255)                                                                                                                       gTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAcatcat            >  2:548836/1‑140 (MQ=255)                                                                                                                       gTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGAT‑‑GCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATgc          >  1:666554/1‑140 (MQ=255)                                                                                                                        ttCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATg           >  1:1184623/1‑140 (MQ=255)                                                                                                                         tCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATgc          >  2:534504/1‑140 (MQ=255)                                                                                                                          cgcgcgGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATgca         <  2:827325/140‑1 (MQ=255)                                                                                                                                 aaTTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCAGCAAACg  <  2:115378/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCAGCAAACG  >  NC_000913/1140650‑1140917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑