Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,292,624	A→G	V100A (GTT→GCT)	hns ←	DNA‑binding transcriptional dual regulator H‑NS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,292,624	0	A	G	100.0%	127.7 / NA	35	V100A (GTT→GCT)	hns	DNA‑binding transcriptional dual regulator H‑NS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/13);  total (22/13)

AGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATTGCTGC  >  NC_000913/1292491‑1292749                                                                                                                                      |                                                                                                                              aGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCg                                                                                                                             <  2:700951/136‑1 (MQ=255) aGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCg                                                                                                                             >  1:700951/1‑136 (MQ=255)  gTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCt                                                                                                                        >  2:694509/1‑140 (MQ=255)             aaaaGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCg                                                                                                                             >  2:413181/1‑124 (MQ=255)             aaaaGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCg                                                                                                             <  1:516020/140‑1 (MQ=255)               aaGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGa                                                                                                           >  2:93805/1‑140 (MQ=255)                    tattGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTg                                                                                                      >  2:744498/1‑140 (MQ=255)                    tattGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGC‑TTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGa                                                                                                     >  1:47920/1‑140 (MQ=255)                                aGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGc                                                                                          >  1:1188609/1‑140 (MQ=255)                                  gAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTCGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCtt                                                                                        >  1:882748/1‑140 (MQ=255)                                       cgtcgAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTg                                                                                   >  1:295892/1‑140 (MQ=255)                                            aGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGa                                                                              >  1:727888/1‑140 (MQ=255)                                                         gCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGgcagca                                                                 >  1:790963/1‑140 (MQ=255)                                                          cTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCaa                                                                >  1:797306/1‑140 (MQ=255)                                                               ccATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTa                                                           >  1:155141/1‑140 (MQ=255)                                                                  ttGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTc                                                        >  2:356149/1‑140 (MQ=255)                                                                   tGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTca                                                       >  1:1009414/1‑140 (MQ=255)                                                                    gCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTcag                                                      >  2:905825/1‑140 (MQ=255)                                                                             aTTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             <  2:155141/140‑1 (MQ=255)                                                                             aTTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             <  2:1188609/140‑1 (MQ=255)                                                                             aTTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             <  1:905825/140‑1 (MQ=255)                                                                                      ggAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAAt                                    <  2:47920/140‑1 (MQ=255)                                                                                      cgAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAAt                                    <  2:882748/139‑1 (MQ=255)                                                                                        aGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             <  1:931935/129‑1 (MQ=255)                                                                                        aGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             >  2:931935/1‑129 (MQ=255)                                                                                        aGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATAc                                  <  2:936845/140‑1 (MQ=255)                                                                                                 ttGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGa                         >  2:33807/1‑140 (MQ=255)                                                                                                             ggTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTcgc             <  2:797306/140‑1 (MQ=255)                                                                                                              gTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTAATCAGCAGTTCGTTCGGGTCAATACCGTCa                             >  1:439463/1‑123 (MQ=255)                                                                                                              gTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTAATCAGCAGTTCGTTCGGGTCAATACCGTCa                             <  2:439463/123‑1 (MQ=255)                                                                                                               ttttAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGa           <  2:295892/140‑1 (MQ=255)                                                                                                               ttttAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGa           <  1:33807/140‑1 (MQ=255)                                                                                                                   aGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATAtt       >  2:175764/1‑140 (MQ=255)                                                                                                                        cGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATtgctgc  >  2:51349/1‑140 (MQ=255)                                                                                                                        cGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATtgctgc  >  1:895492/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                              AGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATTGCTGC  >  NC_000913/1292491‑1292749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑