Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,827	A→G	intergenic (+160/‑4)	ymiC → / → acnA	protein YmiC/aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,827	0	A	G	100.0%	222.2 / NA	60	intergenic (+160/‑4)	ymiC/acnA	protein YmiC/aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/34);  total (26/34)

TTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGT  >  NC_000913/1335691‑1335951                                                                                                                                         |                                                                                                                             ttGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCt                                                                                                                           <  2:254953/140‑1 (MQ=255)   gTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTAt                                                                                                                         >  1:65743/1‑140 (MQ=255)         tgctttgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATgtcgtc                                                                                                                   <  2:577555/140‑1 (MQ=255)         tgctttgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATgtcgtc                                                                                                                   >  1:342414/1‑140 (MQ=255)           ctttgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCaa                                                                                                                 >  1:758022/1‑140 (MQ=255)              tgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAAccc                                                                                                              <  2:758022/140‑1 (MQ=255)                  acaAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACg                                                                                                          <  2:1024857/140‑1 (MQ=255)                   caAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACga                                                                                                         <  1:1122997/140‑1 (MQ=255)                            tcctcTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGt                                                                                                <  1:1060647/140‑1 (MQ=255)                            tcctcTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGt                                                                                                <  2:342414/140‑1 (MQ=255)                             cctcTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTa                                                                                               <  2:924688/140‑1 (MQ=255)                             cctcTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTa                                                                                               <  2:65743/140‑1 (MQ=255)                                ctTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAgg                                                                                            >  1:210801/1‑140 (MQ=255)                                ctTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAgg                                                                                            >  2:949271/1‑140 (MQ=255)                                 ttttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGa                                                                                           <  2:844480/140‑1 (MQ=255)                                   ttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGaca                                                                                         <  2:508145/140‑1 (MQ=255)                                   ttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGaca                                                                                         <  2:908645/140‑1 (MQ=255)                                   ttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGaca                                                                                         <  2:782098/140‑1 (MQ=255)                                    tATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGacac                                                                                        >  2:875112/1‑140 (MQ=255)                                      tCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGt                                                                                      >  2:517402/1‑140 (MQ=255)                                             gggTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTGAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGc                                                                               <  2:126238/140‑1 (MQ=255)                                             gggTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGc                                                                               >  1:126238/1‑140 (MQ=255)                                               gttgttATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCa                                                                             >  1:699955/1‑140 (MQ=255)                                                   ttATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGa                                                                         <  1:923402/140‑1 (MQ=255)                                                     aTCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATa                                                                       >  2:148112/1‑140 (MQ=255)                                                     aTCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATa                                                                       <  1:285908/140‑1 (MQ=255)                                                     aTCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATa                                                                       <  2:845624/140‑1 (MQ=255)                                                           tCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACtt                                                                 <  1:148112/140‑1 (MQ=255)                                                           tCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACtt                                                                 <  2:1128230/140‑1 (MQ=255)                                                           tCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACtt                                                                 <  1:1195665/140‑1 (MQ=255)                                                              ttACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAcc                                                              >  2:712925/1‑140 (MQ=255)                                                              ttACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAcc                                                              <  1:949271/140‑1 (MQ=255)                                                                    gATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAc                                                               >  1:807360/1‑133 (MQ=255)                                                                    gATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAc                                                               <  2:807360/133‑1 (MQ=255)                                                                                aTCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGc                                            >  1:356640/1‑140 (MQ=255)                                                                                 tCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCt                                           <  2:562783/140‑1 (MQ=255)                                                                                   tttAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtg                                         <  2:745436/140‑1 (MQ=255)                                                                                           tCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCtt                                          >  2:98917/1‑131 (MQ=255)                                                                                           tCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCtt                                          <  1:98917/131‑1 (MQ=255)                                                                                           tCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTAGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTaaa                                 <  1:1134194/140‑1 (MQ=255)                                                                                            cACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAAt                                >  2:216709/1‑140 (MQ=255)                                                                                             aCCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATc                               >  1:1202529/1‑140 (MQ=255)                                                                                             aCCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATc                               >  1:520381/1‑140 (MQ=255)                                                                                              cccTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCa                              <  1:606169/140‑1 (MQ=255)                                                                                                 tGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTg                           <  2:815061/140‑1 (MQ=255)                                                                                                   aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCa                              >  1:1019146/1‑135 (MQ=255)                                                                                                   aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCa                              <  2:1019146/135‑1 (MQ=255)                                                                                                   aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTggg                         <  1:875112/140‑1 (MQ=255)                                                                                                   aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTggg                         >  2:35672/1‑140 (MQ=255)                                                                                                         aTCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGatat                   >  1:699391/1‑140 (MQ=255)                                                                                                           cAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCa                 <  1:35672/140‑1 (MQ=255)                                                                                                             gggCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCAcc               >  2:905505/1‑140 (MQ=255)                                                                                                                cTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt            <  1:905505/140‑1 (MQ=255)                                                                                                                cTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt            <  2:699391/140‑1 (MQ=255)                                                                                                                  tCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCt          >  1:756238/1‑140 (MQ=255)                                                                                                                   cGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGa                      >  1:633398/1‑127 (MQ=255)                                                                                                                   cGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGa                      <  2:633398/127‑1 (MQ=255)                                                                                                                        cccTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCaa    >  1:966760/1‑140 (MQ=255)                                                                                                                        cccTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCaa    >  2:1011179/1‑140 (MQ=255)                                                                                                                          cTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGt  >  1:155166/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                             TTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGT  >  NC_000913/1335691‑1335951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑