Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	244,404	T→C	G26G (GGT→GGC)	yafJ →	putative amidotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	244,404	0	T	C	100.0%	198.8 / NA	54	G26G (GGT→GGC)	yafJ	putative amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/25);  total (29/25)

AAAATTCTTAAATCAATCTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGTGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAACTTGTCCAGGACTACCCGATAAAATC  >  NC_000913/244267‑244538                                                                                                                                          |                                                                                                                                       aaaaTTCTTAAATCAATCTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCgtggcgg                                                                                                                                      <  2:225147/140‑1 (MQ=255) aaaaTTCTTAAATCAATCTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCgtggcgg                                                                                                                                      <  2:1121978/140‑1 (MQ=255)   aaTTCTTAAATCAATCTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGCGCGCCAACGTGCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCgtggcggtg                                                                                                                                    >  2:871060/1‑140 (MQ=255)           aatcaatcTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCggg                                                                                                                            <  1:810922/140‑1 (MQ=255)           aatcaatcTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCggg                                                                                                                            <  2:920102/140‑1 (MQ=255)           aatcaatcTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCggg                                                                                                                            <  2:430106/140‑1 (MQ=255)           aatcaatcTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCggg                                                                                                                            <  1:856286/140‑1 (MQ=255)           aatcaatcTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCggg                                                                                                                            <  1:536164/140‑1 (MQ=255)           aatcaatcTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCggg                                                                                                                            <  2:578779/140‑1 (MQ=255)            atcaatcTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGc                                                                                                                           <  1:753647/140‑1 (MQ=255)               aatcTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCcac                                                                                                                        <  2:265915/140‑1 (MQ=255)                atcTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCcaca                                                                                                                       >  1:615397/1‑140 (MQ=255)                atcTTAGCCGGGGGCCATGCGCTCCCTCTGTTGTGGAGGTCACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCTCCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCcaca                                                                                                                       <  2:1091814/140‑1 (MQ=255)                          ggggCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGc                                                                                                             <  1:613090/140‑1 (MQ=255)                           gggCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCt                                                                                                            >  1:208886/1‑140 (MQ=255)                            ggCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTg                                                                                                           <  1:623130/140‑1 (MQ=255)                             gCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGTGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGGACCGGGCCACATAAAAATGGGTgg                                                                                                          >  1:933133/1‑140 (MQ=255)                               cATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTgggg                                                                                                        >  2:371349/1‑140 (MQ=255)                               cATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAAATGGCTgggg                                                                                                        >  2:941828/1‑140 (MQ=255)                                 tGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCa                                                                                                      <  2:208886/140‑1 (MQ=255)                                 tGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCa                                                                                                      >  1:251332/1‑140 (MQ=255)                                        ccGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCtt                                                                                               >  1:17309/1‑140 (MQ=255)                                        ccGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGGGGGACCGGGCCACATAAAGATGGGTGGGGCATTACCtt                                                                                               >  2:1137011/1‑140 (MQ=255)                                            tgttgtGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTAc                                                                                           <  1:1137011/140‑1 (MQ=255)                                                  ggAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCCCATAAAGATGGCTGGGGCATTACCTTTTACGAAggg                                                                                     >  2:321362/1‑139 (MQ=255)                                                      gTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAg                                                                                 >  1:57475/1‑140 (MQ=255)                                                      gTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAg                                                                                 >  1:373656/1‑140 (MQ=255)                                                        tACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACg                                                                                          <  2:725643/129‑1 (MQ=255)                                                        tACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACg                                                                                          >  1:1218302/1‑129 (MQ=255)                                                        tACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACg                                                                                          <  2:1218302/129‑1 (MQ=255)                                                        tACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACg                                                                                          >  1:725643/1‑129 (MQ=255)                                                        tACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGc                                                                               >  2:863612/1‑140 (MQ=255)                                                              tgtgCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGc                                                                         >  2:158755/1‑140 (MQ=255)                                                              tgtgCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGc                                                                         <  2:615397/140‑1 (MQ=255)                                                              tgtgCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGc                                                                         >  2:410423/1‑140 (MQ=255)                                                              tgtgCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTAAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGc                                                                         <  2:1021105/140‑1 (MQ=255)                                                               gtgCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCg                                                                          <  2:799130/138‑1 (MQ=255)                                                               gtgCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCg                                                                          >  1:799130/1‑138 (MQ=255)                                                               gtgCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGca                                                                        <  1:863612/140‑1 (MQ=255)                                                                      ttGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTaa                                                                 <  2:57475/140‑1 (MQ=255)                                                                      ttGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTaa                                                                 <  2:113072/140‑1 (MQ=255)                                                                       tGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTaaa                                                                >  2:573010/1‑140 (MQ=255)                                                                          tCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGAt                                                             >  1:237438/1‑140 (MQ=255)                                                                                 gAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAAc                                                      <  2:237438/140‑1 (MQ=255)                                                                                        aaCGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAAcc                                                     >  1:928742/1‑134 (MQ=255)                                                                                        aaCGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAAcc                                                     <  2:928742/134‑1 (MQ=255)                                                                                                       atCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGc                                >  2:348214/1‑140 (MQ=255)                                                                                                       atCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGc                                >  2:832298/1‑140 (MQ=255)                                                                                                           gCTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCaaa                            <  2:251332/140‑1 (MQ=255)                                                                                                            cTTTAGTTTCACCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAAc                           >  2:902890/1‑140 (MQ=255)                                                                                                             tttAGTTTCACCGGGCTTGTATAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAACt                          >  1:908798/1‑140 (MQ=255)                                                                                                                      aCCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAAc                           <  2:715248/130‑1 (MQ=255)                                                                                                                      aCCGGGCTTGTACAGCGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAAc                           >  1:715248/1‑130 (MQ=255)                                                                                                                                     cGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAACTTGTCCAGGACTACCCGATAAAATc  >  1:117469/1‑140 (MQ=255)                                                                                                                                     cGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAACTTGTCCAGGACTACCCGATAAAATc  >  1:1212276/1‑140 (MQ=255)                                                                                                                                     cGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAACTTGTCCAGGACTACCCGATAAAATc  >  1:1220651/1‑140 (MQ=255)                                                                                                                                     cGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAACTTGTCCAGGACTACCCGATAAAATc  >  1:827733/1‑140 (MQ=255)                                                                                                                                     cGTGGCGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAACTTGTCCAGGACTACCCGATAAAATc  >  2:135056/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AAAATTCTTAAATCAATCTTCGCCGGGGGCCATGCGCTCCCGCTGTTGTGGAGGTTACCCATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGGCTTGTACAGCGTGGTGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATCGCCAAACTTGTCCAGGACTACCCGATAAAATC  >  NC_000913/244267‑244538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑