| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,387,478 | A→G | R253R (AGA→AGG) | tyrR → | DNA‑binding transcriptional dual regulator TyrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,387,478 | 0 | A | G | 95.8% | 170.0 / ‑1.7 | 48 | R253R (AGA→AGG) | tyrR | DNA‑binding transcriptional dual regulator TyrR |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (21/25); minor base T (1/1); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TCAGCCCGAAAATGAAGCATGTTGTCGAACAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGAGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACGGTGGTTCGGTGCTGTT > NC_000913/1387345‑1387609 | tCAGCCCGAAAATGAAGCATGTTGTCGAACAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAgggcgggc < 1:1061106/140‑1 (MQ=255) cccGAAAATGAAGCATGTTGTCGAACAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAAc > 2:295552/1‑140 (MQ=255) cccGAAAATGAAGCATGTTGTCGAACAGGCGCAGAAACTGACGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGTGCGGGCAAAc > 1:640978/1‑140 (MQ=255) aGCATGTTGTCGAACAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGgcg < 2:91822/140‑1 (MQ=255) aGCATGTTGTCGAACAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGgcg > 2:622092/1‑140 (MQ=255) gtCGAACAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGGTGATTACGGGTGACACAGGTACAGGTAAAGATTTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACtg < 2:1147633/140‑1 (MQ=255) tCGAACAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACtgt > 2:930979/1‑140 (MQ=255) aaCAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCg > 2:480469/1‑140 (MQ=255) aCAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGt < 1:295552/140‑1 (MQ=255) aCAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGt < 2:425616/140‑1 (MQ=255) gcgcAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCtat > 2:936176/1‑140 (MQ=255) aaaCTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGa > 1:835900/1‑140 (MQ=255) ggCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATg < 2:528958/140‑1 (MQ=255) cgcgcCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTg < 2:1038852/140‑1 (MQ=255) cgcTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTg < 1:622092/140‑1 (MQ=255) ctgctgATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGtt < 1:936176/140‑1 (MQ=255) tgATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGt < 1:272288/140‑1 (MQ=255) aTTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCa < 2:261834/140‑1 (MQ=255) tACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATg > 2:1230557/1‑140 (MQ=255) cGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCt > 1:9126/1‑140 (MQ=255) gggTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTc > 1:994704/1‑140 (MQ=255) gTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCg > 2:825558/1‑140 (MQ=255) gTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCg > 1:183967/1‑140 (MQ=255) acacAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCggaa > 2:1202834/1‑140 (MQ=255) acacAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCggaa > 2:437582/1‑140 (MQ=255) aggtacaggtaAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCggaaggga < 1:198967/140‑1 (MQ=255) gtaAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGa > 2:1163723/1‑140 (MQ=255) gtaAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGa > 2:847459/1‑140 (MQ=255) gtaAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGa < 1:1202834/140‑1 (MQ=255) aaaGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGAtt > 1:72297/1‑140 (MQ=255) aTCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCttt < 2:72297/140‑1 (MQ=255) tctTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAg < 2:334413/140‑1 (MQ=255) ctTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGTGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAg < 2:386029/139‑1 (MQ=255) ctTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGTGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAg > 1:386029/1‑139 (MQ=255) ctTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGc > 2:327733/1‑140 (MQ=255) ctTTGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGc > 2:474416/1‑140 (MQ=255) tttGCCTACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCa > 1:817632/1‑140 (MQ=255) tACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGaa < 1:1015782/140‑1 (MQ=255) tACGCCTGCCATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGaa < 1:825558/140‑1 (MQ=255) gCCTGCCATCAGGCAAGCCCCAGTGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACgg < 2:640978/140‑1 (MQ=255) ccATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGaa < 2:297715/132‑1 (MQ=255) ccATCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGaa > 1:297715/1‑132 (MQ=255) tCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACGGTGGTTCgg < 1:847459/140‑1 (MQ=255) tCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACGGTGGTTCgg < 1:1163723/140‑1 (MQ=255) tCAGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACGGTGGTTCgg < 1:474416/140‑1 (MQ=255) aGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACGGTGGTTCGGTg < 2:994704/140‑1 (MQ=255) aGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACGGTGGTTCGGTg < 1:930979/140‑1 (MQ=255) aGGCAAGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACGGTGGTTCGGTg < 1:1230557/140‑1 (MQ=255) aGCCCCAGGGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACGGTGGTTCGGTGCTGtt < 2:1050712/140‑1 (MQ=255) | TCAGCCCGAAAATGAAGCATGTTGTCGAACAGGCGCAGAAACTGGCGATGCTAAGCGCGCCGCTGCTGATTACGGGTGACACAGGTACAGGTAAAGATCTCTTTGCCTACGCCTGCCATCAGGCAAGCCCCAGAGCGGGCAAACCTTACCTGGCGCTGAACTGTGCGTCTATACCGGAAGATGCGGTCGAGAGTGAACTGTTTGGTCATGCTCCGGAAGGGAAGAAAGGATTCTTTGAGCAGGCGAACGGTGGTTCGGTGCTGTT > NC_000913/1387345‑1387609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |