Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,425,452	A→G	S26G (AGT→GGT)	ynaK →	Rac prophage; ParB‑like nuclease domain‑containing protein YnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,425,452	0	A	G	100.0%	226.7 / NA	61	S26G (AGT→GGT)	ynaK	Rac prophage; ParB‑like nuclease domain‑containing protein YnaK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/28);  total (33/28)

AACACCTCCGTCCCCAGTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACAGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATT  >  NC_000913/1425315‑1425587                                                                                                                                          |                                                                                                                                        aaCACCTCCGTCCCCAGTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGt                                                                                                                                       >  1:387559/1‑140 (MQ=255)  acacCTCCGTCCCCAGTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTa                                                                                                                                      >  1:920996/1‑140 (MQ=255)      cTCCGTCCCCAGTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGa                                                                                                                                  <  2:114036/140‑1 (MQ=255)        ccGTCCCCAGTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGc                                                                                                                                >  1:1091575/1‑140 (MQ=255)         cGTCCCCAGTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCa                                                                                                                               >  2:374452/1‑140 (MQ=255)         cGTCCCCAGTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCa                                                                                                                               <  2:826822/140‑1 (MQ=255)         cGTCCCCAGTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCa                                                                                                                               <  2:387559/140‑1 (MQ=255)                aGTAGCACTGGCTGCTGGGGTGCGTTTTATTCGTAAAGCAAGGCTGTATGGGCGAGAAATTAAAGATAGTCAATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGca                                                                                                                        >  2:858097/1‑140 (MQ=255)                 gTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGcac                                                                                                                       <  1:119173/140‑1 (MQ=255)                 gTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGcac                                                                                                                       <  2:715182/140‑1 (MQ=255)                  tAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGcaca                                                                                                                      >  1:881657/1‑140 (MQ=255)                   aGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACaa                                                                                                                     >  1:601070/1‑140 (MQ=255)                      aCTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTg                                                                                                                  <  1:458061/140‑1 (MQ=255)                          gctgctGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAg                                                                                                              >  1:751793/1‑140 (MQ=255)                               tgGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGt                                                                                                         >  1:886355/1‑140 (MQ=255)                                ggggTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTa                                                                                                        <  2:125574/140‑1 (MQ=255)                                    tGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTaa                                                                                                    >  1:679778/1‑140 (MQ=255)                                      cGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGc                                                                                                  >  1:1008179/1‑140 (MQ=255)                                        ttttATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCaa                                                                                                <  2:601070/140‑1 (MQ=255)                                          ttATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAAtt                                                                                              <  2:1008179/140‑1 (MQ=255)                                             ttCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCgg                                                                                           <  2:886355/140‑1 (MQ=255)                                              tCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGc                                                                                          <  2:751793/140‑1 (MQ=255)                                                   aaGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGAc                                                                                     <  2:920996/140‑1 (MQ=255)                                                          gCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCg                                                                              >  2:557444/1‑140 (MQ=255)                                                              tATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCATAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGc                                                                          >  2:1040163/1‑140 (MQ=255)                                                              tATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGc                                                                          >  1:1075553/1‑140 (MQ=255)                                                                    cGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCt                                                                         <  1:1201299/135‑1 (MQ=255)                                                                    cGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCt                                                                         >  2:1201299/1‑135 (MQ=255)                                                                    cGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTg                                                                    >  1:454762/1‑140 (MQ=255)                                                                    cGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTg                                                                    >  1:603118/1‑140 (MQ=255)                                                                        aaaTTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGa                                                                >  1:143513/1‑140 (MQ=255)                                                                                     tCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAatt                                                   >  1:73416/1‑140 (MQ=255)                                                                                     tCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAatt                                                   >  1:286825/1‑140 (MQ=255)                                                                                        aTCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAattatt                                                <  1:571437/140‑1 (MQ=255)                                                                                           gCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCg                                             >  1:181879/1‑140 (MQ=255)                                                                                              cATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGt                                          <  2:181879/140‑1 (MQ=255)                                                                                               aTTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTc                                         >  1:814559/1‑140 (MQ=255)                                                                                               aTTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTc                                         <  2:1156355/140‑1 (MQ=255)                                                                                                ttACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCa                                        <  2:881657/140‑1 (MQ=255)                                                                                                ttACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCa                                        <  1:649802/140‑1 (MQ=255)                                                                                                     aGAATTGTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTc                                   <  1:374452/140‑1 (MQ=255)                                                                                                           gTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACgg                                     <  1:1126906/132‑1 (MQ=255)                                                                                                           gTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACgg                                     >  2:1126906/1‑132 (MQ=255)                                                                                                           gTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTa                             >  2:677883/1‑140 (MQ=255)                                                                                                           gTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTa                             >  2:953218/1‑140 (MQ=255)                                                                                                           gTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTa                             >  1:910814/1‑140 (MQ=255)                                                                                                           gTCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTa                             >  1:959158/1‑140 (MQ=255)                                                                                                            tCACCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTAt                            <  1:613814/140‑1 (MQ=255)                                                                                                              aCCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTc                                   >  2:839378/1‑131 (MQ=255)                                                                                                              aCCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTc                                   <  1:839378/131‑1 (MQ=255)                                                                                                              aCCGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGCAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATgg                          <  2:1075553/140‑1 (MQ=255)                                                                                                               ccGTATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATggc                         <  2:814559/140‑1 (MQ=255)                                                                                                                  tATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTAt                            >  2:624234/1‑134 (MQ=255)                                                                                                                  tATGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTAt                            <  1:624234/134‑1 (MQ=255)                                                                                                                    tGCGCACAACGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTg                    >  2:580705/1‑140 (MQ=255)                                                                                                                           aaCGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCt             <  2:910814/140‑1 (MQ=255)                                                                                                                             cGCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCa           >  1:507140/1‑140 (MQ=255)                                                                                                                              gCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCaa          >  1:31545/1‑140 (MQ=255)                                                                                                                              gCCAGGACGCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCaa          >  2:503612/1‑140 (MQ=255)                                                                                                                                 aGGACGCACGGTACTGAGCAGGTGGCCCAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGAGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAAt       <  2:1091575/140‑1 (MQ=255)                                                                                                                                      gCACGGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGAtt  <  1:1150163/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AACACCTCCGTCCCCAGTAGCACTGGCTGCTGGGGTGCGTTTTATTCATAAAGCAAGGCTGTATGAGCGAGAAATTAAAGATAGTCTATCGCCCATTACAAGAATTGTCACCGTATGCGCACAACGCCAGGACGCACAGTACTGAGCAGGTGGCACAACTGGTAGAAAGTATTAAGCAATTCGGCTGGACTAATCCGGTGCTGATTGACGAAAAGGGCGAAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATT  >  NC_000913/1425315‑1425587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑