Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,441,545	A→G	C67R (TGT→CGT)	hslJ ←	lipoprotein implicated in Novobiocin resistance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,441,545	0	A	G	100.0%	208.4 / NA	56	C67R (TGT→CGT)	hslJ	lipoprotein implicated in Novobiocin resistance
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/24);  total (32/24)

CCTTCTTTCAGCATTTCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACACATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACA  >  NC_000913/1441409‑1441672                                                                                                                                         |                                                                                                                                ccTTCTTTCAGCATTTCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCAt                                                                                                                              >  1:946821/1‑140 (MQ=255)  cttcttTCAGCATTTCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCAtg                                                                                                                             >  2:509723/1‑140 (MQ=255)               ttCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAatca                                                                                                                >  1:179826/1‑140 (MQ=255)                      aTGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCAtttt                                                                                                         <  1:36211/140‑1 (MQ=255)                         gtgtTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTc                                                                                                      >  2:268009/1‑140 (MQ=255)                         gtgtTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTc                                                                                                      >  2:509727/1‑140 (MQ=255)                          tgtTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCa                                                                                                     <  2:179826/140‑1 (MQ=255)                           gtTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCAc                                                                                                    <  1:964432/140‑1 (MQ=255)                                cGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAg                                                                                               >  2:632809/1‑140 (MQ=255)                                    ttCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGa                                                                                           >  2:438634/1‑140 (MQ=255)                                              tGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCgg                                                                                 >  2:1014713/1‑140 (MQ=255)                                                cGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGAt                                                                               >  1:729968/1‑140 (MQ=255)                                                cGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGAt                                                                               >  1:1105223/1‑140 (MQ=255)                                                      aGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTAt                                                                         <  1:632809/140‑1 (MQ=255)                                                        cgcACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCg                                                                       <  2:729968/140‑1 (MQ=255)                                                             atcatACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCACCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGt                                                                  <  2:546778/140‑1 (MQ=255)                                                               catACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCa                                                                <  1:268009/140‑1 (MQ=255)                                                                 tACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACg                                                              <  2:946821/140‑1 (MQ=255)                                                                      gTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCtt                                                         <  1:509727/140‑1 (MQ=255)                                                                       tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGt                                                                  >  2:697678/1‑130 (MQ=255)                                                                       tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGt                                                                  <  1:697678/130‑1 (MQ=255)                                                                       tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  1:886533/1‑140 (MQ=255)                                                                       tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  2:552769/1‑140 (MQ=255)                                                                       tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  2:104805/1‑140 (MQ=255)                                                                       tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  2:1069560/1‑140 (MQ=255)                                                                       tCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTa                                                        >  2:942669/1‑140 (MQ=255)                                                                         aTTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTAcc                                                      >  2:935399/1‑140 (MQ=255)                                                                           tGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGt                                                    >  2:899095/1‑140 (MQ=255)                                                                                gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               <  1:88062/140‑1 (MQ=255)                                                                                gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               <  1:509723/140‑1 (MQ=255)                                                                                gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               <  1:552769/140‑1 (MQ=255)                                                                                gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               >  1:1028441/1‑140 (MQ=255)                                                                                gCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCAATTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACg                                               <  1:1015419/140‑1 (MQ=255)                                                                                     ttGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTc                                          >  2:386997/1‑140 (MQ=255)                                                                                      tGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTcc                                         >  1:1198007/1‑140 (MQ=255)                                                                                           gTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGcac                                    <  2:886533/140‑1 (MQ=255)                                                                                            tCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGcaca                                   >  1:947286/1‑140 (MQ=255)                                                                                              aGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACaaa                                 >  2:1197317/1‑140 (MQ=255)                                                                                               gTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAg                                <  1:1014713/140‑1 (MQ=255)                                                                                                ttCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGc                               >  1:833660/1‑140 (MQ=255)                                                                                                  cACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCga                             >  2:296340/1‑140 (MQ=255)                                                                                                  cACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCga                             <  1:942669/140‑1 (MQ=255)                                                                                                   aCCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCgat                            >  2:401832/1‑140 (MQ=255)                                                                                                             aGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAg                  <  2:1105223/140‑1 (MQ=255)                                                                                                             aGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAg                  <  1:899095/140‑1 (MQ=255)                                                                                                             aGTTTGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCATTCCAGCACAAAGCGATGATGCTGTAg                  <  1:935399/140‑1 (MQ=255)                                                                                                               tttGCCTTCACCGCTAAAGCGGTTACGCATGCTGCTGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCt                >  1:501984/1‑140 (MQ=255)                                                                                                               tttGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCg                              <  2:979101/126‑1 (MQ=255)                                                                                                               tttGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCg                              >  1:979101/1‑126 (MQ=255)                                                                                                               tttGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCt                >  2:1186222/1‑140 (MQ=255)                                                                                                                 tGCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGt              >  2:69928/1‑140 (MQ=255)                                                                                                                  gCCTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTATGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGtt             <  1:386997/140‑1 (MQ=255)                                                                                                                   ccTTCACCGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTc            <  2:947286/140‑1 (MQ=255)                                                                                                                         ccGCTAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGt      >  2:947760/1‑140 (MQ=255)                                                                                                                             tAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACa  >  2:846328/1‑140 (MQ=255)                                                                                                                             tAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACa  <  1:104805/140‑1 (MQ=255)                                                                                                                             tAAAGCGGTTACGCATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACa  >  2:372638/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                CCTTCTTTCAGCATTTCGCTAATGGTGTTATCGAGTTCATTAAGCTGCGGGTTAGCGCACATCATACGGGTCATTGCCAGCCCTTTGGCTGTCAGTTCACCATTAGACAGTTTGCCTTCACCGCTAAAGCGGTTACACATGCTGCCGGAAATCATCATTTTTTCACCAAAGCTGATTTCTGGCGGATTTTTATCGCTGGTCACGGGCTTACCGTTTACGCTTTCCAGCACAAAGCGATGATGCTGTAGCTGTTCTGGCGTAACA  >  NC_000913/1441409‑1441672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑