Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	251,815	G→A	R306R (CGG→CGA)	dinB →	DNA polymerase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	251,815	0	G	A	100.0%	176.7 / NA	50	R306R (CGG→CGA)	dinB	DNA polymerase IV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/22);  total (28/22)

TATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATT  >  NC_000913/251683‑251949                                                                                                                                     |                                                                                                                                       tATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATa                                                                                                                                 >  1:149612/1‑140 (MQ=255)    cGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAg                                                                                                                              <  1:197491/140‑1 (MQ=255)       gCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTg                                                                                                                           <  2:149612/140‑1 (MQ=255)          gCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATc                                                                                                                        >  1:372914/1‑140 (MQ=255)                     aaCTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCAcc                                                                                                             >  2:979601/1‑140 (MQ=255)                           aaCGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGt                                                                                                       <  2:180428/140‑1 (MQ=255)                           aaCGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGt                                                                                                       <  1:979601/140‑1 (MQ=255)                             cGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTaa                                                                                                     <  1:473140/140‑1 (MQ=255)                                cGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAAc                                                                                                  <  2:48549/140‑1 (MQ=255)                                  tCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCt                                                                                                <  1:114363/140‑1 (MQ=255)                                  tCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCt                                                                                                >  2:634991/1‑140 (MQ=255)                                          aGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGaa                                                                                        >  1:580631/1‑140 (MQ=255)                                                aaCCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAAcgccgc                                                                                  >  1:1213520/1‑140 (MQ=255)                                                         tACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGgcgc                                                                         <  1:1101816/140‑1 (MQ=255)                                                          aCTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGcgcg                                                                        <  2:372914/140‑1 (MQ=255)                                                              aTTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGgtgt                                                                    >  2:220576/1‑140 (MQ=255)                                                                 gCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCg                                                                 >  2:813350/1‑140 (MQ=255)                                                                  cTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCggcgggcg                                                                          <  1:452893/130‑1 (MQ=255)                                                                  cTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCggcgggcg                                                                          >  2:452893/1‑130 (MQ=255)                                                                  cTCGCCAGGAGGCGAATTAAAAGGTCGACGATTTTCGGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGGGTGCGt                                                                >  2:1106588/1‑140 (MQ=255)                                                                   tCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTc                                                               <  1:294446/140‑1 (MQ=255)                                                                         gggggTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCtggtgg                                                         >  2:805439/1‑140 (MQ=255)                                                                          ggggTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTggg                                                        >  1:725054/1‑140 (MQ=255)                                                                          ggggTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTggg                                                        >  2:351753/1‑140 (MQ=255)                                                                                 aaTTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCAt                                                 <  1:634991/140‑1 (MQ=255)                                                                                      aaGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGAc                                            <  1:220576/140‑1 (MQ=255)                                                                                      aaGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGAc                                            >  2:420449/1‑140 (MQ=255)                                                                                           cgacgaTTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGc                                       >  2:101728/1‑140 (MQ=255)                                                                                            gacgaTTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCt                                      >  1:21172/1‑140 (MQ=255)                                                                                            gacgaTTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCt                                      >  2:411586/1‑140 (MQ=255)                                                                                             acgaTTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCtt                                     <  1:119171/140‑1 (MQ=255)                                                                                                 ttttCAGCAAACCACCCAGGAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGAcc                                 <  1:411586/140‑1 (MQ=255)                                                                                                                   ggAGCACGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAAc               <  1:430292/140‑1 (MQ=255)                                                                                                                         cGTCTGGCTGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGc         >  2:418970/1‑140 (MQ=255)                                                                                                                         cGTCTGGCTGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGc         <  1:418970/140‑1 (MQ=255)                                                                                                                         cGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGGGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGc         >  2:814004/1‑140 (MQ=255)                                                                                                                         cGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGc         >  1:725547/1‑140 (MQ=255)                                                                                                                         cGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGc         <  2:725547/140‑1 (MQ=255)                                                                                                                         cGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGc         >  1:236275/1‑140 (MQ=255)                                                                                                                         cGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGc         >  2:421652/1‑140 (MQ=255)                                                                                                                         cGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGc         <  1:813350/140‑1 (MQ=255)                                                                                                                         cGTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGATGc         <  1:63834/140‑1 (MQ=255)                                                                                                                          gTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCt        >  2:780891/1‑140 (MQ=255)                                                                                                                          gTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCt        >  1:1008430/1‑140 (MQ=255)                                                                                                                          gTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCt        <  2:225786/140‑1 (MQ=255)                                                                                                                          gTCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGGGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCCCAAATGGAAAGGCAACTGGTggt        >  2:884639/1‑138 (MQ=255)                                                                                                                           tCTGGCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTg       <  1:884639/140‑1 (MQ=255)                                                                                                                              ggCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGa    >  1:539659/1‑140 (MQ=255)                                                                                                                               gCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGAt   >  1:721126/1‑140 (MQ=255)                                                                                                                               gCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGAt   >  1:811100/1‑140 (MQ=255)                                                                                                                               gCCGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGAt   >  2:159353/1‑140 (MQ=255)                                                                                                                                ccGCGACTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGAtt  <  2:1008430/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATT  >  NC_000913/251683‑251949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑