Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,917,898	A→G	N130S (AAC→AGC)	yebT →	intermembrane transport protein YebT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,917,898	0	A	G	100.0%	161.0 / NA	44	N130S (AAC→AGC)	yebT	intermembrane transport protein YebT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

TTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAG  >  NC_000913/1917763‑1918033                                                                                                                                        |                                                                                                                                        ttCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCtat                                                                                                                                     <  1:976149/140‑1 (MQ=255) ttCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCtat                                                                                                                                     <  2:1210932/140‑1 (MQ=255)        gATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCa                                                                                                                              <  2:808038/140‑1 (MQ=255)         aTTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCat                                                                                                                             >  1:918218/1‑140 (MQ=255)         aTTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCat                                                                                                                             >  1:1031609/1‑140 (MQ=255)         aTTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCat                                                                                                                             >  2:1230957/1‑140 (MQ=255)         aTTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGAC‑CCCTCGTCGGTGGGAGCTATATCGGCatg                                                                                                                            >  2:589517/1‑140 (MQ=255)             aaGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCatgatg                                                                                                                         <  1:170935/140‑1 (MQ=255)             aaGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCatgatg                                                                                                                         >  2:696233/1‑140 (MQ=255)              aGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGc                                                                                                                        <  1:514327/140‑1 (MQ=255)              aGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATCATGc                                                                                                                        <  2:1031609/140‑1 (MQ=255)                  aaGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCggg                                                                                                                    <  2:910571/140‑1 (MQ=255)                  aaGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCggg                                                                                                                    <  1:190132/140‑1 (MQ=255)                   aGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTGTATCGGCATGATGCCGggt                                                                                                                   >  1:721274/1‑140 (MQ=255)                    ggTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGggta                                                                                                                  >  2:477125/1‑140 (MQ=255)                        aGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGggtaaagg                                                                                                              >  2:720919/1‑140 (MQ=255)                        aGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGggtaaagg                                                                                                              >  2:893897/1‑140 (MQ=255)                         gCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGggtaaaggt                                                                                                             <  2:918218/140‑1 (MQ=255)                            tCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGggtaaaggtaaa                                                                                                          >  1:684905/1‑140 (MQ=255)                               aGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTTGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAagag                                                                                                       <  1:1021732/140‑1 (MQ=255)                               aGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAagag                                                                                                       >  2:89698/1‑140 (MQ=255)                               aGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAagag                                                                                                       <  2:606124/140‑1 (MQ=255)                                      tatGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATc                                                                                                >  2:318336/1‑140 (MQ=255)                                                gcgctgcgcGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGc                                                                                      <  1:89698/140‑1 (MQ=255)                                                   ctgcgcGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACt                                                                                   >  1:852566/1‑140 (MQ=255)                                                          aaGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGAC‑CCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATAccc                                                                           <  1:589517/140‑1 (MQ=255)                                                           agagACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTGTATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATAccc                                                                           <  2:721274/140‑1 (MQ=255)                                                           agagACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATAccc                                                                           <  1:70730/140‑1 (MQ=255)                                                                cTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCg                                                                      >  1:588150/1‑140 (MQ=255)                                                                  cAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGaa                                                                    <  1:477125/140‑1 (MQ=255)                                                                              gTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGa                                                        <  2:916239/140‑1 (MQ=255)                                                                              gTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGa                                                        <  1:1230957/140‑1 (MQ=255)                                                                               tGACGCCAAGAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGc                                                            >  1:1006983/1‑135 (MQ=255)                                                                               tGACGCCAAGAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGc                                                            <  2:1006983/135‑1 (MQ=255)                                                                               tGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGAc                                                       >  2:84052/1‑140 (MQ=255)                                                                                 aCGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGACTGGACaa                                                     <  2:852566/140‑1 (MQ=255)                                                                                       aaaGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGa                                               <  1:320726/140‑1 (MQ=255)                                                                                       aaaGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGa                                               <  1:1016519/140‑1 (MQ=255)                                                                                                                     gACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtct                 >  1:1107124/1‑140 (MQ=255)                                                                                                                     gACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtct                 >  1:586696/1‑140 (MQ=255)                                                                                                                     gACGCCCTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtct                 >  2:1130465/1‑140 (MQ=255)                                                                                                                          ccTCGTCGGTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGtt            >  2:151282/1‑140 (MQ=255)                                                                                                                                 ggTGGGAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGaa     <  1:84052/140‑1 (MQ=255)                                                                                                                                    gggAGCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGTCCCCGATCTCGGTTCGCTGAACAg  >  1:251251/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAG  >  NC_000913/1917763‑1918033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑