Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,005,518	T→C	S72S (AGT→AGC)	fliS →	flagellar biosynthesis protein FliS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,005,518	0	T	C	100.0%	258.8 / NA	70	S72S (AGT→AGC)	fliS	flagellar biosynthesis protein FliS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/27);  total (43/27)

CTGGTCACCATGCTATTTGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGTCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATA  >  NC_000913/2005381‑2005654                                                                                                                                          |                                                                                                                                         cTGGTCACCATGCTATTTGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCt                                                                                                                                        >  1:28584/1‑140 (MQ=255)     tCACCATGCTATTTGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTtgat                                                                                                                                    <  2:1087967/140‑1 (MQ=255)        ccATGCTATTTGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGaa                                                                                                                                 <  2:261458/140‑1 (MQ=255)           tGCTATTTGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAagag                                                                                                                              >  2:1037050/1‑140 (MQ=255)             cTATTTGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAagagag                                                                                                                            <  1:756395/140‑1 (MQ=255)               aTTTGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGaa                                                                                                                                 >  1:276044/1‑133 (MQ=255)               aTTTGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGaa                                                                                                                                 <  2:276044/133‑1 (MQ=255)                tttGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAagagag                                                                                                                            <  1:461781/137‑1 (MQ=255)                tttGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAagagag                                                                                                                            >  2:461781/1‑137 (MQ=255)                        aGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAAc                                                                                                                 >  1:1124630/1‑140 (MQ=255)                         gTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACt                                                                                                                >  2:1206430/1‑140 (MQ=255)                              gAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAAccc                                                                                                           >  2:938166/1‑140 (MQ=255)                                    aCTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACt                                                                                                     >  1:679567/1‑140 (MQ=255)                                    aCTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACt                                                                                                     >  2:545282/1‑140 (MQ=255)                                       ggTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGa                                                                                                  <  2:28584/140‑1 (MQ=255)                                          tAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTg                                                                                               <  2:123923/140‑1 (MQ=255)                                             agcgagcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGctc                                                                                            >  1:441123/1‑140 (MQ=255)                                             agcgagcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGctc                                                                                            >  2:796375/1‑140 (MQ=255)                                              gcgagcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGctct                                                                                           <  1:938166/140‑1 (MQ=255)                                              gcgagcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGctct                                                                                           <  1:1037050/140‑1 (MQ=255)                                                gagcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCttt                                                                                         >  2:996885/1‑140 (MQ=255)                                                 agcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTta                                                                                        <  2:1209859/140‑1 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTg                                                                                               <  1:496434/132‑1 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTg                                                                                               >  2:496434/1‑132 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGct                                                                                             <  2:207695/134‑1 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGct                                                                                             >  1:207695/1‑134 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTtat                                                                                       >  2:35653/1‑140 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTtat                                                                                       >  2:842026/1‑140 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTtat                                                                                       >  2:701533/1‑140 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTtat                                                                                       >  1:995700/1‑140 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTtat                                                                                       >  1:204119/1‑140 (MQ=255)                                                  gcCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGCGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTtat                                                                                       >  1:338518/1‑140 (MQ=255)                                                   ccTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTtata                                                                                      >  1:562446/1‑140 (MQ=255)                                                    cTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAg                                                                                     <  1:996885/140‑1 (MQ=255)                                                       tttATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGct                                                                                             >  1:450452/1‑129 (MQ=255)                                                       tttATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGct                                                                                             <  2:450452/129‑1 (MQ=255)                                                               ggACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCa                                                                          >  1:184366/1‑140 (MQ=255)                                                                 acaacaATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTtat                                                                                       <  2:374731/125‑1 (MQ=255)                                                                 acaacaATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTtat                                                                                       >  1:374731/1‑125 (MQ=255)                                                                 acaacaATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAgg                                                                        >  1:478378/1‑140 (MQ=255)                                                                 acaacaATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAgg                                                                        >  2:569429/1‑140 (MQ=255)                                                                     caaTCAGTAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCt                                                                    <  2:356973/140‑1 (MQ=255)                                                                     caaTCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCt                                                                    >  1:612018/1‑140 (MQ=255)                                                                     caaTCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCt                                                                    <  2:220049/140‑1 (MQ=255)                                                                               ggCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGc                                                          >  2:971305/1‑140 (MQ=255)                                                                               ggCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGc                                                          >  1:358771/1‑140 (MQ=255)                                                                               ggCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGc                                                          >  1:615766/1‑140 (MQ=255)                                                                                   aaGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAAt                                                      <  2:1124630/140‑1 (MQ=255)                                                                                      gCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTa                                                   <  2:562446/140‑1 (MQ=255)                                                                                           tctTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCaa                                              >  2:810945/1‑140 (MQ=255)                                                                                                gTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATg                                         >  1:431678/1‑140 (MQ=255)                                                                                                      aGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTa                                                   >  1:1089416/1‑124 (MQ=255)                                                                                                      aGCGATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCg                                   >  1:707690/1‑140 (MQ=255)                                                                                                      aGCGATCAACATCATTCAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTa                                                   <  2:1089416/124‑1 (MQ=255)                                                                                                         gATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAg                                >  1:612040/1‑140 (MQ=255)                                                                                                         gATCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAg                                >  2:684205/1‑140 (MQ=255)                                                                                                          aTCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAAc                                             <  2:1197831/126‑1 (MQ=255)                                                                                                          aTCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAAc                                             >  1:1197831/1‑126 (MQ=255)                                                                                                           tCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTc                              >  1:337145/1‑140 (MQ=255)                                                                                                           tCAACATCATTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTc                              >  2:922909/1‑140 (MQ=255)                                                                                                               catcatTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCgaag                          <  2:357920/140‑1 (MQ=255)                                                                                                                   atTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGt                      <  2:707690/140‑1 (MQ=255)                                                                                                                   atTGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGt                      <  1:810945/140‑1 (MQ=255)                                                                                                                     tGAGAACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTgg                    <  2:995700/140‑1 (MQ=255)                                                                                                                      gagaACGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGa                   >  1:554267/1‑140 (MQ=255)                                                                                                                         aaCGGACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGc                >  2:632144/1‑140 (MQ=255)                                                                                                                             gACTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTa            <  1:796375/140‑1 (MQ=255)                                                                                                                              aCTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCATGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa           >  1:802486/1‑140 (MQ=255)                                                                                                                              aCTGCGGGTGAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTaa           >  1:393111/1‑140 (MQ=255)                                                                                                                                       gAGCCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAata  <  1:545282/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CTGGTCACCATGCTATTTGATGGAGTGCTGAGCGCACTGGTTAGAGCGAGCCTGTTTATGCAGGACAACAATCAGCAAGGCAAAGGCGTCTCTTTGTCAAAAGCGATCAACATCATTGAGAACGGACTGCGGGTGAGTCTTGATGAAGAGAGCAAAGACGAACTAACCCAAAACTTGATTGCTCTTTATAGCTATATGGTCAGGCGCTTGCTGCAAGCCAATTTACGCAACGATGTCTCCGCAGTCGAAGAAGTGGAAGCATTAATGCGCAATA  >  NC_000913/2005381‑2005654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑