Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,326,014	G→A	G420D (GGC→GAC)	atoE →	short chain fatty acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,326,014	0	G	A	100.0%	219.6 / NA	62	G420D (GGC→GAC)	atoE	short chain fatty acid transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/32);  total (30/32)

AGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAGTGCGGTACGTACTGCTA  >  NC_000913/2325877‑2326148                                                                                                                                          |                                                                                                                                       aGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGTCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACt                                                                                                                                      >  1:698685/1‑140 (MQ=255)     cAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTg                                                                                                                                  <  2:562745/140‑1 (MQ=255)     cAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTg                                                                                                                                  <  1:138826/140‑1 (MQ=255)             gggcgctgATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAGCCATTCTGGGCGCTGCCAGCACTGGCAATCGCC‑GACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGc                                                                                                                         >  2:316007/1‑140 (MQ=255)                  ctgATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTg                                                                                                                     <  2:221533/140‑1 (MQ=255)                              aaTCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAGCCATTCTGGGCGCTGCCAGCACTGGCAATCGCC‑GACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGgtg                                                                                                        <  1:316007/140‑1 (MQ=255)                               aTCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGGCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGgtg                                                                                                        >  2:843442/1‑140 (MQ=255)                               aTCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGcc                                                                                                                        <  2:263970/124‑1 (MQ=255)                               aTCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGcc                                                                                                                        >  1:263970/1‑124 (MQ=255)                               aTCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGgtg                                                                                                        >  1:732239/1‑140 (MQ=255)                                 cGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTc                                                                                                      >  1:1098623/1‑140 (MQ=255)                                    tAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCAtt                                                                                                   <  2:801554/140‑1 (MQ=255)                                    tAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGCCAtt                                                                                                   >  2:910819/1‑140 (MQ=255)                                         gCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGt                                                                                              <  2:431078/140‑1 (MQ=255)                                          ccaTCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTc                                                                                             >  1:130727/3‑140 (MQ=255)                                          cGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTc                                                                                             >  1:810084/1‑140 (MQ=255)                                            aTCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCAt                                                                                           <  2:732239/140‑1 (MQ=255)                                            aTCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCAt                                                                                           <  2:706284/140‑1 (MQ=255)                                              cGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTg                                                                                         >  2:1053266/1‑140 (MQ=255)                                                 cTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGtt                                                                                      <  2:847524/140‑1 (MQ=255)                                                   aCGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCAt                                                                                           >  1:1173581/1‑133 (MQ=255)                                                   aCGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCAt                                                                                           <  2:1173581/133‑1 (MQ=255)                                                         aGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTg                                                                              >  1:624328/1‑140 (MQ=255)                                                         aGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTg                                                                              >  2:614318/1‑140 (MQ=255)                                                         aGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTg                                                                              >  1:880635/1‑140 (MQ=255)                                                         aGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTg                                                                              >  1:896578/1‑140 (MQ=255)                                                         aGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGCCATTTTCGTCATTGGTTTAACGCTg                                                                              >  1:1134567/1‑140 (MQ=255)                                                           cAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTATCGTCATTGGTTTAACGCTGtt                                                                            >  1:873242/1‑140 (MQ=255)                                                                  tGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACgg                                                                     <  2:989902/140‑1 (MQ=255)                                                                    aaCATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCa                                                                   <  1:657773/140‑1 (MQ=255)                                                                    aaCATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCa                                                                   <  1:1053266/140‑1 (MQ=255)                                                                         ggCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCt                                                              >  1:616901/1‑140 (MQ=255)                                                                         ggCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCt                                                              >  2:162822/1‑140 (MQ=255)                                                                          gCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACTTCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTa                                                             >  1:1046175/1‑140 (MQ=255)                                                                          gCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTa                                                             <  1:100568/140‑1 (MQ=255)                                                                          gCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTa                                                             >  2:706872/1‑140 (MQ=255)                                                                          gCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTa                                                             >  2:1038847/1‑140 (MQ=255)                                                                          gCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTa                                                             >  1:811652/1‑140 (MQ=255)                                                                           cacaACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACgg                                                                     <  2:61099/131‑1 (MQ=255)                                                                           cacaACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACgg                                                                     >  1:61099/1‑131 (MQ=255)                                                                           cacaACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTAc                                                            >  2:278729/1‑140 (MQ=255)                                                                               aCCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAAc                                                        <  2:917651/140‑1 (MQ=255)                                                                               aCCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAAc                                                        <  1:162822/140‑1 (MQ=255)                                                                                 cATTCTGGGCGCTGTCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAg                                                      <  2:698685/140‑1 (MQ=255)                                                                                  aTTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGa                                                     <  2:349950/140‑1 (MQ=255)                                                                                  aTTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGa                                                     <  1:464221/140‑1 (MQ=255)                                                                                   ttCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGaa                                                    <  2:1098623/140‑1 (MQ=255)                                                                                        gcgattGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAat                                               >  2:254451/6‑140 (MQ=255)                                                                                           gcTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAAtata                                            <  1:1227345/140‑1 (MQ=255)                                                                                                cAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATg                                       <  1:278729/140‑1 (MQ=255)                                                                                                       ggCAATCGCCGGACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAAtt                                <  1:48851/140‑1 (MQ=255)                                                                                                                 ggACTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGt                      <  1:706872/140‑1 (MQ=255)                                                                                                                    cTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAg                   >  2:79711/1‑140 (MQ=255)                                                                                                                    cTCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAg                   <  2:316305/140‑1 (MQ=255)                                                                                                                     tCGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAGt                  <  2:1134567/140‑1 (MQ=255)                                                                                                                      cGGTGTCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAGTg                 <  1:1186428/140‑1 (MQ=255)                                                                                                                        gtgtCCGCGACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAGTGCg               <  2:75261/140‑1 (MQ=255)                                                                                                                          gtCCGCGACTTCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAGTGCGgt             <  2:1046175/140‑1 (MQ=255)                                                                                                                             cgcgACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAGTGCGgt             >  2:1160040/1‑137 (MQ=255)                                                                                                                             cgcgACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAGTGCGgt             <  1:1160040/137‑1 (MQ=255)                                                                                                                               cgACATCATGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCTTCGTCAGTGCGgtacgta        >  1:685585/1‑140 (MQ=255)                                                                                                                                     catGGACTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAGTGCGGTACGTACTGCTa  >  1:1051946/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AGCCCAGGCGCTGGGCGCTGATCTCGGTAAATCGGTAATGGCGATCGCCTACGGCGAGCAATGGATGAACATGGCACAACCATTCTGGGCGCTGCCAGCACTGGCAATCGCCGGACTCGGTGTCCGCGACATCATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCTACAAACAGAAGGAATATAAAATGAAAAATTGTGTCATCGTCAGTGCGGTACGTACTGCTA  >  NC_000913/2325877‑2326148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑