Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	305,321	C→T	V499V (GTG→GTA)	ecpD ←	fimbrial adhesin EcpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	305,321	0	C	T	97.7%	288.9 / ‑1.9	85	V499V (GTG→GTA)	ecpD	fimbrial adhesin EcpD
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (41/42);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

ACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAAC  >  NC_000913/305186‑305456                                                                                                                                        |                                                                                                                                        aCGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACtt                                                                                                                                     >  1:905630/1‑140 (MQ=255)  cGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTc                                                                                                                                    >  1:478958/1‑140 (MQ=255)  cGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTc                                                                                                                                    >  2:579061/1‑140 (MQ=255)  cGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTCCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTc                                                                                                                                    >  1:212543/1‑140 (MQ=255)   gCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCg                                                                                                                                   <  1:1030548/140‑1 (MQ=255)   gCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCg                                                                                                                                   >  2:1030548/1‑140 (MQ=255)     cACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGcc                                                                                                                                 <  1:467457/140‑1 (MQ=255)      aCGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCa                                                                                                                                >  2:52709/1‑140 (MQ=255)       cGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCag                                                                                                                               >  1:1063262/1‑140 (MQ=255)       cGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCag                                                                                                                               >  2:272485/1‑140 (MQ=255)        gTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCaga                                                                                                                              >  1:954320/1‑140 (MQ=255)             cTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGatat                                                                                                                         <  1:850421/140‑1 (MQ=255)                  cATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTc                                                                                                                    <  1:1102547/140‑1 (MQ=255)                     gAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCAc                                                                                                                 >  1:966946/1‑140 (MQ=255)                      aaTCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACg                                                                                                                >  2:1117167/1‑140 (MQ=255)                         ctctcCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGgtg                                                                                                             <  2:507540/140‑1 (MQ=255)                         ctctcCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGgtg                                                                                                             <  1:579061/140‑1 (MQ=255)                         ctctcCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGgtg                                                                                                             <  1:272485/140‑1 (MQ=255)                             ccTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGt                                                                                                         >  1:1200839/1‑140 (MQ=255)                             ccTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGt                                                                                                         <  2:954320/140‑1 (MQ=255)                                   gcgcTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACca                                                                                                   <  2:1200839/140‑1 (MQ=255)                                      cTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACca                                                                                                   <  1:998999/137‑1 (MQ=255)                                      cTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACca                                                                                                   >  2:998999/1‑137 (MQ=255)                                      cTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAg                                                                                                >  1:1044644/1‑140 (MQ=255)                                      cTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAg                                                                                                >  1:287587/1‑140 (MQ=255)                                       tGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAgc                                                                                               <  2:478958/140‑1 (MQ=255)                                        gACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAgcg                                                                                              >  1:1207973/1‑140 (MQ=255)                                         aCTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAgcgc                                                                                             <  2:769679/140‑1 (MQ=255)                                           ttCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCAt                                                                                           <  1:10256/140‑1 (MQ=255)                                           ttCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCAt                                                                                           <  2:643447/140‑1 (MQ=255)                                              gCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCgg                                                                                        >  1:554160/1‑140 (MQ=255)                                                   aCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCata                                                                                   >  1:715154/1‑140 (MQ=255)                                                   aCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCata                                                                                   >  1:289515/1‑140 (MQ=255)                                                          gTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGcc                                                                            <  2:733586/140‑1 (MQ=255)                                                          gTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGcc                                                                            <  2:160363/140‑1 (MQ=255)                                                            tATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAg                                                                          <  2:287587/140‑1 (MQ=255)                                                             atcatcTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGc                                                                         <  2:905630/140‑1 (MQ=255)                                                                   tACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTatcat                                                                   <  2:1063262/140‑1 (MQ=255)                                                                     ccGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTatcatc                                                                  >  1:253644/1‑139 (MQ=255)                                                                     ccGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTatcatc                                                                  <  2:253644/139‑1 (MQ=255)                                                                        tACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAc                                                              >  1:1096164/1‑140 (MQ=255)                                                                        tACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAc                                                              >  1:1124129/1‑140 (MQ=255)                                                                        tACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAc                                                              <  2:289515/140‑1 (MQ=255)                                                                        tACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAc                                                              >  2:331766/1‑140 (MQ=255)                                                                        tACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAc                                                              >  1:14162/1‑140 (MQ=255)                                                                        tACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAc                                                              <  2:715154/140‑1 (MQ=255)                                                                        tACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAc                                                              >  1:507789/1‑140 (MQ=255)                                                                          cGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAccc                                                            >  1:1062974/1‑140 (MQ=255)                                                                          cGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAccc                                                            <  2:966946/140‑1 (MQ=255)                                                                           gCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCg                                                           <  1:297159/140‑1 (MQ=255)                                                                           gCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCAGCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCg                                                           <  2:212543/140‑1 (MQ=255)                                                                            cAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCgg                                                          <  1:832655/140‑1 (MQ=255)                                                                            cAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCgg                                                          >  2:698020/1‑140 (MQ=255)                                                                              gCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCa                                                        <  1:331766/140‑1 (MQ=255)                                                                                 aaaTGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCg                                                     <  2:507789/140‑1 (MQ=255)                                                                                 aaaTGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCg                                                     <  2:994820/140‑1 (MQ=255)                                                                                   aTGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTa                                                   <  1:1117167/140‑1 (MQ=255)                                                                                      gCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGt                                                <  1:52709/140‑1 (MQ=255)                                                                                         ttATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGtttt                                             >  1:1187341/1‑140 (MQ=255)                                                                                            tCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGt                                          >  1:1013475/1‑140 (MQ=255)                                                                                                 tGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTc                                     >  2:876797/1‑140 (MQ=255)                                                                                                   gAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCg                                   >  2:182514/1‑140 (MQ=255)                                                                                                     aTCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGtt                                 <  1:219711/140‑1 (MQ=255)                                                                                                     aTCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGtt                                 <  1:689640/140‑1 (MQ=255)                                                                                                      tCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTg                                >  1:465326/1‑140 (MQ=255)                                                                                                      tCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTg                                >  2:531160/1‑140 (MQ=255)                                                                                                      tCGAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTg                                >  2:273642/1‑140 (MQ=255)                                                                                                        gAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCg                              <  1:295448/140‑1 (MQ=255)                                                                                                        gAAAATTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCg                              <  2:1124129/140‑1 (MQ=255)                                                                                                            aTTTGACTGTGGTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGta                          <  2:465326/140‑1 (MQ=255)                                                                                                                      ggTCTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGaaa                <  2:1207973/140‑1 (MQ=255)                                                                                                                         cTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAgtg             <  2:1062974/140‑1 (MQ=255)                                                                                                                         cTTATCCATCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAgtg             <  2:1044644/140‑1 (MQ=255)                                                                                                                                aTCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCgg      <  2:1096164/140‑1 (MQ=255)                                                                                                                                 tCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCggg     >  2:877389/1‑140 (MQ=255)                                                                                                                                 tCTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCggg     >  2:1069305/1‑140 (MQ=255)                                                                                                                                  cTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCgg      <  2:612703/138‑1 (MQ=255)                                                                                                                                  cTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCgg      >  1:612703/1‑138 (MQ=255)                                                                                                                                  cTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGa    >  1:781438/1‑140 (MQ=255)                                                                                                                                  cTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGa    >  1:670915/1‑140 (MQ=255)                                                                                                                                  cTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGa    >  1:591361/1‑140 (MQ=255)                                                                                                                                  cTGCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGa    >  1:490372/1‑140 (MQ=255)                                                                                                                                    gCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGcc        <  1:798319/134‑1 (MQ=255)                                                                                                                                    gCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGcc        >  2:798319/1‑134 (MQ=255)                                                                                                                                    gCCCTACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGATGCGGGTAATAAAGGAAAGTGTCGCCGGGAAc  <  2:1013475/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGACTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAAC  >  NC_000913/305186‑305456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑