Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,767	A→G	K250K (AAA→AAG)	arnD →	putative 4‑deoxy‑4‑formamido‑L‑arabinose‑phosphoundecaprenol deformylase ArnD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,767	0	A	G	100.0%	192.6 / NA	52	K250K (AAA→AAG)	arnD	putative 4‑deoxy‑4‑formamido‑L‑arabinose‑phosphoundecaprenol deformylase ArnD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/24);  total (28/24)

TCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAGT  >  NC_000913/2370632‑2370902                                                                                                                                        |                                                                                                                                        tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     <  1:101993/140‑1 (MQ=255) tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     <  1:902366/140‑1 (MQ=255) tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     <  1:827668/140‑1 (MQ=255) tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     >  2:827668/1‑140 (MQ=255) tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     <  1:331407/140‑1 (MQ=255)  cGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAgcgcgc                                                                                                                                    >  2:399338/1‑140 (MQ=255)   gggATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCa                                                                                                                                   <  2:715291/140‑1 (MQ=255)    ggATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAg                                                                                                                                  >  1:1191532/1‑140 (MQ=255)          gAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAgg                                                                                                                            >  2:50237/1‑140 (MQ=255)               cAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAgg                                                                                                                            >  2:883689/1‑135 (MQ=255)               cAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAgg                                                                                                                            <  1:883689/135‑1 (MQ=255)                          aaCGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTg                                                                                                            <  1:399338/140‑1 (MQ=255)                           aCGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGc                                                                                                           <  2:304280/140‑1 (MQ=255)                              gttggttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCt                                                                                                        >  1:934396/1‑140 (MQ=255)                               ttggttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCtt                                                                                                       <  1:1146365/140‑1 (MQ=255)                                tggttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCttt                                                                                                      >  2:122632/1‑140 (MQ=255)                                  gttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTaa                                                                                                    >  2:968857/1‑140 (MQ=255)                                  gttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTaa                                                                                                    <  1:50237/140‑1 (MQ=255)                                      cTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGa                                                                                                <  2:1149581/140‑1 (MQ=255)                                       tCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGaa                                                                                               <  2:934396/140‑1 (MQ=255)                                       tCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGaa                                                                                               <  1:968857/140‑1 (MQ=255)                                           ccGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTg                                                                                                            <  1:822838/123‑1 (MQ=255)                                           ccGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTg                                                                                                            >  2:822838/1‑123 (MQ=255)                                              cATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGc                                                                                                  >  1:75138/1‑130 (MQ=255)                                              cATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGc                                                                                                  <  2:75138/130‑1 (MQ=255)                                                tCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAAc                                                                                              <  1:373573/132‑1 (MQ=255)                                                tCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAAc                                                                                              >  2:373573/1‑132 (MQ=255)                                                 ccTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCg                                                                                                 >  2:286004/1‑128 (MQ=255)                                                 ccTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCg                                                                                                 <  1:286004/128‑1 (MQ=255)                                                  cTGCGAGATAAAGGCACGCCGG‑TTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCagag                                                                                   <  1:100578/140‑1 (MQ=255)                                                              ggCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCt                                                                        <  2:361964/140‑1 (MQ=255)                                                                  cGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGa                                                                    >  1:440054/1‑140 (MQ=255)                                                                            taCCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTc                                                          <  2:440054/140‑1 (MQ=255)                                                                                aTTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCgg                                                      <  2:1191532/140‑1 (MQ=255)                                                                                 ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                                     >  1:72325/1‑140 (MQ=255)                                                                                 ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                                     >  2:185737/1‑140 (MQ=255)                                                                                 ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                                     >  2:197241/1‑140 (MQ=255)                                                                                 ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                                     >  2:442882/1‑140 (MQ=255)                                                                                         gAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGc                                             >  1:1046615/1‑140 (MQ=255)                                                                                            gTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCgg                                          >  2:980498/1‑140 (MQ=255)                                                                                               gAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACg                                       >  1:939451/1‑140 (MQ=255)                                                                                                aaGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGt                                      >  2:554784/1‑140 (MQ=255)                                                                                                    cTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAg                                  >  1:458931/1‑140 (MQ=255)                                                                                                       cgcTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAggct                               >  2:548121/1‑140 (MQ=255)                                                                                                            aTCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGgctggctg                          >  2:586294/1‑140 (MQ=255)                                                                                                                 cATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTg                     >  2:762206/1‑140 (MQ=255)                                                                                                                 cATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTg                     >  1:375815/1‑140 (MQ=255)                                                                                                                     aTTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCcaa                 <  1:273344/140‑1 (MQ=255)                                                                                                                          gtgGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAAt            >  1:835791/1‑140 (MQ=255)                                                                                                                           tgGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAAtt           <  2:939451/140‑1 (MQ=255)                                                                                                                                 tcctcAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGt     >  1:369071/1‑140 (MQ=255)                                                                                                                                   ctcAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAg   >  1:62943/1‑140 (MQ=255)                                                                                                                                   ctcAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAg   >  1:1198586/1‑140 (MQ=255)                                                                                                                                    tcAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAGt  <  2:1046615/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAGT  >  NC_000913/2370632‑2370902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑