Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,447,083	T→C	T131A (ACC→GCC)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,447,083	0	T	C	100.0%	144.1 / NA	47	T131A (ACC→GCC)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/24);  total (23/24)

TTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGTTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCGCACTGTAATCCTGAGAGCGCTG  >  NC_000913/2446948‑2447206                                                                                                                                        |                                                                                                                            tttGCTCGACCTGCGACCAGTCTTTGATATCCAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTcg                                                                                                                         <  1:263840/140‑1 (MQ=255)                    gTCTTTGATATCCAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGCTTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAACAACGTCCAc                                                                                                      >  1:468926/1‑140 (MQ=255)                     tCTTTGATATCCAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCAcc                                                                                                     >  2:1097311/1‑140 (MQ=255)                               ccAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGa                                                                                              <  2:183516/137‑1 (MQ=255)                               ccAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGa                                                                                              >  1:183516/1‑137 (MQ=255)                               ccAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATaa                                                                                           >  1:1037741/1‑140 (MQ=255)                               ccAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATaa                                                                                           >  2:344791/1‑140 (MQ=255)                               ccAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATaa                                                                                           >  1:752457/1‑140 (MQ=255)                               ccAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATaa                                                                                           >  1:119175/1‑140 (MQ=255)                                 aGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATc                                                                                         >  1:919538/1‑140 (MQ=255)                                   cGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATcgc                                                                                       >  2:651905/1‑140 (MQ=255)                                    ggAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATcgcg                                                                                      <  2:428933/140‑1 (MQ=255)                                      aaTGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCa                                                                                    <  2:1042013/140‑1 (MQ=255)                                        tggcGCCCTCCTGGTTCAGGCACCCCCGGATTTCAATGCGAAATTGCGCGGGGATATTTTTTTTGGCAATAGCTCCTGCCGCCGCGCGGTTGGCGGTTTGGTGGGCGGAGGAGCGTCCGCCGCCGCGATAATCGCGCAGa                                                                                  <  2:468926/137‑1 (MQ=255)                                        tGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATcgcg                                                                                      >  2:883146/1‑136 (MQ=255)                                        tGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATcgcg                                                                                      <  1:883146/136‑1 (MQ=255)                                        tGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGa                                                                                  >  1:1146921/1‑140 (MQ=255)                                        tGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCCCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGa                                                                                  <  1:352004/140‑1 (MQ=255)                                                  cTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTAttttt                                                                        >  1:437856/1‑140 (MQ=255)                                                    gggTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATA‑TTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTAttt                                                                          >  2:714795/1‑135 (MQ=255)                                                    gggTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATA‑TTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTAttt                                                                          <  1:714795/135‑1 (MQ=255)                                                       tCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCg                                                                   <  1:265015/140‑1 (MQ=255)                                                        cAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGt                                                                  <  2:1146921/140‑1 (MQ=255)                                                          ggCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTc                                                                    >  2:147471/1‑136 (MQ=255)                                                          ggCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTc                                                                    <  1:147471/136‑1 (MQ=255)                                                          ggCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAg                                                                >  1:1041910/1‑140 (MQ=255)                                                               ccACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCAGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTa                                                           >  2:346191/1‑140 (MQ=255)                                                                cACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTaa                                                          <  2:437856/140‑1 (MQ=255)                                                                     ttttCAATCCCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGc                                                     <  2:440150/139‑1 (MQ=255)                                                                     ttttCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGc                                                     <  2:752457/139‑1 (MQ=255)                                                                     aTTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGc                                                     <  2:16779/140‑1 (MQ=255)                                                                     aTTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGc                                                     <  1:1106831/140‑1 (MQ=255)                                                                     aTTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGc                                                     >  1:16779/1‑140 (MQ=255)                                                                     aTTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGc                                                     <  1:651905/140‑1 (MQ=255)                                                                                 aaTTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGcc                                               >  1:1038139/1‑134 (MQ=255)                                                                                 aaTTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGcc                                               <  2:1038139/134‑1 (MQ=255)                                                                                 aaTTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACg                                         >  1:243578/1‑140 (MQ=255)                                                                                 aaTTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACg                                         >  2:764745/1‑140 (MQ=255)                                                                                       tcGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAAc                                   >  1:860784/1‑140 (MQ=255)                                                                                        cGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg                                  <  1:1097311/140‑1 (MQ=255)                                                                                            gagaTATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCt                              <  1:764745/140‑1 (MQ=255)                                                                                              gaTATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGTCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTa                            >  2:303602/1‑140 (MQ=255)                                                                                                  ttttttGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCg                        <  2:243578/140‑1 (MQ=255)                                                                                                  ttttttGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCg                        <  1:344791/140‑1 (MQ=255)                                                                                                  ttttttGGCAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCg                        <  2:860784/140‑1 (MQ=255)                                                                                                          cAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCg                        >  2:18176/1‑132 (MQ=255)                                                                                                          cAATAGCTCCTGCCGCCACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCg                        <  1:18176/132‑1 (MQ=255)                                                                                                                     gccgccACGCGCATGTCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCGCACTGTAATCCTGAGAgcg     <  1:303602/140‑1 (MQ=255)                                                                                                                     gccgccACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCGCACTGTAATCCTGAGAgcg     <  2:1041910/140‑1 (MQ=255)                                                                                                                        gccACGCGCATGGCGGCTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCGCACTGTAATCCTGAGAGCGCTg  >  2:930666/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                            TTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTTTTGGCAATAGCTCCTGCCGCCACGCGCATGGCGGTTTCGCGGGCGGAAGAACGTCCACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACGTCCTTAATCGCACTGTAATCCTGAGAGCGCTG  >  NC_000913/2446948‑2447206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑