Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,476,771	T→C	T287A (ACA→GCA)	dsdC ←	DNA‑binding transcriptional dual regulator DsdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,476,771	0	T	C	100.0%	197.1 / NA	53	T287A (ACA→GCA)	dsdC	DNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/23);  total (30/23)

TAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAATAA  >  NC_000913/2476634‑2476905                                                                                                                                          |                                                                                                                                       taaattaaatTTGAACTAGCAGGCCGAATAAGGCACTCCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATgcgg                                                                                                                                      <  2:1197790/140‑1 (MQ=255) taaattaaatTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATgcgg                                                                                                                                      >  1:1036542/1‑140 (MQ=255) taaattaaatTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATgcgg                                                                                                                                      <  1:765569/140‑1 (MQ=255)    gttaaattCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGa                                                                                                                                   <  1:216188/139‑1 (MQ=255)    attaaattCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGa                                                                                                                                   <  1:822519/140‑1 (MQ=255)    attaaattCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGa                                                                                                                                   <  1:834761/140‑1 (MQ=255)        aattCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGta                                                                                                                               >  2:1107501/1‑140 (MQ=255)         attCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGtaa                                                                                                                              >  1:292857/1‑140 (MQ=255)                  aGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGa                                                                                                                     <  1:1107501/140‑1 (MQ=255)                  aGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGa                                                                                                                     <  2:106233/140‑1 (MQ=255)                   gCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGAt                                                                                                                    <  1:338480/140‑1 (MQ=255)                          gAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCAttt                                                                                                             <  2:681365/140‑1 (MQ=255)                           aaTAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTc                                                                                                            <  2:411511/140‑1 (MQ=255)                                     acCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCatat                                                                                                  >  2:241577/1‑140 (MQ=255)                                     acCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCatat                                                                                                  >  1:447775/1‑140 (MQ=255)                                                     tttATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGcgac                                                                                  <  2:7831/140‑1 (MQ=255)                                                        aTTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAg                                                                               >  2:1191654/1‑140 (MQ=255)                                                          tCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCt                                                                             <  1:241577/140‑1 (MQ=255)                                                            aTTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTca                                                                           >  2:1175580/1‑140 (MQ=255)                                                                   aGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTgg                                                                    <  1:1191654/140‑1 (MQ=255)                                                                   aGTGGTTTTTACCTGTTATCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTgg                                                                    >  1:660324/1‑140 (MQ=255)                                                                   aGTGGGTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTgg                                                                    >  2:849844/1‑140 (MQ=255)                                                                    gTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACCGGc                                                                   <  2:292857/140‑1 (MQ=255)                                                                      ggTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACCGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCaa                                                                 >  1:1174081/1‑140 (MQ=255)                                                                            tACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCttt                                                           >  1:140385/1‑140 (MQ=255)                                                                                 gTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGt                                                      >  1:1117774/1‑140 (MQ=255)                                                                                 gTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGt                                                      >  2:460179/1‑140 (MQ=255)                                                                                 gTTCTCTTAGCCAGACAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCGAAACGGCGCGACGTGCTCACCACTGGCAAGCCTTATTTGt                                                      >  2:1091736/1‑140 (MQ=255)                                                                                      ctTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTTCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCa                                                                  >  1:1083548/1‑123 (MQ=255)                                                                                      ctTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTTCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCa                                                                  <  2:1083548/123‑1 (MQ=255)                                                                                       ttAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAgg                                                >  2:58668/1‑140 (MQ=255)                                                                                       ttAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAgg                                                <  2:1055073/140‑1 (MQ=255)                                                                                            cAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGttt                                           >  1:16905/1‑140 (MQ=255)                                                                                              gATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTttc                                         <  1:221181/140‑1 (MQ=255)                                                                                               ataataAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACTAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTttct                                        >  1:236971/1‑140 (MQ=255)                                                                                               ataataAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTttct                                        >  1:730717/1‑140 (MQ=255)                                                                                               ataataAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTttct                                        >  1:220523/1‑140 (MQ=255)                                                                                                    aaaCGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGGATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTccc                                    >  2:359393/1‑139 (MQ=255)                                                                                                    aaaCGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGGATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTccc                                    <  1:359393/139‑1 (MQ=255)                                                                                                        gCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGc                               >  2:776531/1‑140 (MQ=255)                                                                                                        gCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGc                               <  2:553456/140‑1 (MQ=255)                                                                                                        gCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGc                               <  2:16905/140‑1 (MQ=255)                                                                                                        gCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGc                               >  1:553456/1‑140 (MQ=255)                                                                                                         cTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGcc                              >  2:84405/1‑140 (MQ=255)                                                                                                         cTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGcc                              >  1:1080264/1‑140 (MQ=255)                                                                                                         cTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGcc                              >  1:646421/1‑140 (MQ=255)                                                                                                         cTTCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGcc                              >  1:992483/1‑140 (MQ=255)                                                                                                           tCAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCAc                            <  2:140385/140‑1 (MQ=255)                                                                                                            cAATTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCAcc                           <  1:58668/140‑1 (MQ=255)                                                                                                             aaTTTTTGGCCACTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCAccc                          <  2:730717/140‑1 (MQ=255)                                                                                                                        aCTGCCTGCCCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACTAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCACCCCAATATGATTc               <  2:236971/140‑1 (MQ=255)                                                                                                                           gcctgccCGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCACCCCAATATGATTCATc            <  1:1202246/140‑1 (MQ=255)                                                                                                                                cccGGTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCACCCCAATATGATTCATCGCGGc       >  1:1093618/1‑140 (MQ=255)                                                                                                                                   ggTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAAt    <  1:776531/140‑1 (MQ=255)                                                                                                                                    gTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAATa   >  2:298114/1‑140 (MQ=255)                                                                                                                                    gTAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAATa   >  1:234741/1‑140 (MQ=255)                                                                                                                                     tAATGCGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAATaa  >  2:1209466/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATCGCCACCCCAATATGATTCATCGCGGCAATAA  >  NC_000913/2476634‑2476905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑