Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,656,816	G→A	R90C (CGC→TGC)	fdx ←	reduced ferredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,656,816	0	G	A	100.0%	192.1 / NA	54	R90C (CGC→TGC)	fdx	reduced ferredoxin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/23);  total (31/23)

CGCATCGTACAGTGCTTCGCCAATTTCGCGGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAG  >  NC_000913/2656683‑2656949                                                                                                                                      |                                                                                                                                      cGCATCGTACAGTGCTTCGCCAATTTCGCGGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTg                                                                                                                                 >  2:502656/1‑140 (MQ=255)              gCTTCGCCAATTTCGCGGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACgg                                                                                                                    >  2:181640/1‑140 (MQ=255)                ttCGCCAATTTCGCGGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCt                                                                                                                  >  2:1198772/1‑140 (MQ=255)                    ccAATTTCGCGGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTcc                                                                                                              <  1:1198772/140‑1 (MQ=255)                       aTTTCGCGGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGc                                                                                                           >  2:717962/1‑140 (MQ=255)                          tCGCGGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTcc                                                                                                        <  2:382985/140‑1 (MQ=255)                          tCGCGGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTcc                                                                                                        <  1:502656/140‑1 (MQ=255)                           cgcgGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCa                                                                                                       <  1:364035/140‑1 (MQ=255)                                                     aTACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATgtcg                                                                             >  1:56626/1‑140 (MQ=255)                                                     aTACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATgtcg                                                                             <  1:181640/140‑1 (MQ=255)                                                           aCCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTcc                                                                       >  1:1226631/1‑140 (MQ=255)                                                               ctGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGct                                                                   >  1:175901/1‑140 (MQ=255)                                                                tGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGctc                                                                  >  1:345781/1‑140 (MQ=255)                                                                tGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGctc                                                                  >  1:417410/1‑140 (MQ=255)                                                                 gTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCGGGCTTTGTCCAGCATGTCGTCTTCCTGctct                                                                 >  2:164589/1‑140 (MQ=255)                                                                 gTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGctct                                                                 >  2:1143667/1‑140 (MQ=255)                                                                  ttAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATgtcg                                                                             <  1:473615/127‑1 (MQ=255)                                                                  ttAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATgtcg                                                                             >  2:473615/1‑127 (MQ=255)                                                                  ttAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTg                                                                >  1:443365/1‑140 (MQ=255)                                                                    aaTGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCGGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAg                                                              <  1:164589/140‑1 (MQ=255)                                                                     aTGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGc                                                             >  1:897104/1‑140 (MQ=255)                                                                      tGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCt                                                            <  1:717962/140‑1 (MQ=255)                                                                          cACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTcc                                                        <  2:897104/140‑1 (MQ=255)                                                                              cgcATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCa                                                    <  1:283715/140‑1 (MQ=255)                                                                                cATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAATGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGt                                                  >  2:236108/1‑140 (MQ=255)                                                                                cATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGt                                                  <  1:892128/140‑1 (MQ=255)                                                                                   ggTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAg                                               >  2:4157/1‑140 (MQ=255)                                                                                       gATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCaa                                           >  1:287651/1‑140 (MQ=255)                                                                                             gtAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCtt                                     >  2:774927/1‑140 (MQ=255)                                                                                              tAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAg                                                   >  1:1206738/1‑125 (MQ=255)                                                                                              tAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAg                                                   <  2:1206738/125‑1 (MQ=255)                                                                                              tAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTc                                    >  1:43586/1‑140 (MQ=255)                                                                                              tAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTc                                    <  1:1143667/140‑1 (MQ=255)                                                                                              tAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTc                                    >  1:1175494/1‑140 (MQ=255)                                                                                              tAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTc                                    >  2:734508/1‑140 (MQ=255)                                                                                               aaCGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCaaaa                                         <  2:1046413/134‑1 (MQ=255)                                                                                               aaCGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCaaaa                                         >  1:1046413/1‑134 (MQ=255)                                                                                               aaCGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCa                                   >  1:178694/1‑140 (MQ=255)                                                                                               aaCGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCa                                   >  2:643024/1‑140 (MQ=255)                                                                                                  gcgGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacga                                >  2:1120146/1‑140 (MQ=255)                                                                                                   cgGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaa                               <  2:443365/140‑1 (MQ=255)                                                                                                   cgGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaa                               <  1:774927/140‑1 (MQ=255)                                                                                                   cgGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaa                               <  2:708438/140‑1 (MQ=255)                                                                                                   cgGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaa                               >  2:247200/1‑140 (MQ=255)                                                                                                   cgGGATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaa                               <  1:4157/140‑1 (MQ=255)                                                                                                      gATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaacga                            >  2:258461/1‑140 (MQ=255)                                                                                                      gATTTCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaacga                            <  2:1226631/140‑1 (MQ=255)                                                                                                        tttCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAATGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATg                          <  1:236108/140‑1 (MQ=255)                                                                                                        tttCGACTACTAAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATg                          <  1:247200/140‑1 (MQ=255)                                                                                                                actaAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCa                  >  2:380753/1‑140 (MQ=255)                                                                                                                actaAATCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCa                  >  1:137946/1‑140 (MQ=255)                                                                                                                   aaaTCTTCGTCGGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAggt               <  1:1120146/140‑1 (MQ=255)                                                                                                                             cgGTAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGca     <  2:287651/140‑1 (MQ=255)                                                                                                                                tAACGCACGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAg  >  1:521470/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      CGCATCGTACAGTGCTTCGCCAATTTCGCGGCTATCGGTCCACTTAAGTCCCATACTAACCTCTGTTAATGCTCACGCGCATGGTTGATAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAG  >  NC_000913/2656683‑2656949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑