Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,703,236	G→A	H51Y (CAT→TAT)	era ←	30S ribosomal subunit maturation GTPase Era

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,703,236	0	G	A	100.0%	134.6 / NA	38	H51Y (CAT→TAT)	era	30S ribosomal subunit maturation GTPase Era
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/21);  total (17/21)

AAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAG  >  NC_000913/2703107‑2703366                                                                                                                                  |                                                                                                                                   aaaTCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCcaca                                                                                                                          <  1:1174467/140‑1 (MQ=255)              aaCATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAg                                                                                                             <  1:1184653/140‑1 (MQ=255)              aaCATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAg                                                                                                             <  1:64433/140‑1 (MQ=255)                     ccAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTg                                                                                                      <  2:641008/140‑1 (MQ=255)                     ccAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTg                                                                                                      >  1:156968/1‑140 (MQ=255)                         tAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGcc                                                                                                  >  1:592379/1‑140 (MQ=255)                            agCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAg                                                                                                             >  2:1007893/1‑126 (MQ=255)                            agCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAg                                                                                                             <  1:1007893/126‑1 (MQ=255)                              ctgctCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCg                                                                                             >  2:684434/1‑140 (MQ=255)                              ctgctCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCg                                                                                             <  1:167453/140‑1 (MQ=255)                                 ctCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGa                                                                                          >  2:329623/1‑140 (MQ=255)                                  tCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGaa                                                                                         <  2:156968/140‑1 (MQ=255)                                   cgccgcTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAg                                                                                        >  2:1074613/1‑140 (MQ=255)                                                atcaGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGAttt                                                                            >  1:968449/1‑139 (MQ=255)                                                atcaGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGAttt                                                                            <  2:968449/139‑1 (MQ=255)                                                 tcaGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTc                                                                          <  1:588111/140‑1 (MQ=255)                                                           aaTGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                                                <  2:592379/140‑1 (MQ=255)                                                             tGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGt                                                              <  1:684434/140‑1 (MQ=255)                                                                   gTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTc                                                        >  1:310705/1‑140 (MQ=255)                                                                    tttttCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                                                >  1:997368/1‑131 (MQ=255)                                                                    tttttCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                                                <  2:997368/131‑1 (MQ=255)                                                                          ttcCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAAtg                                                 <  1:963765/140‑1 (MQ=255)                                                                              atatGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGa                                             <  2:994295/140‑1 (MQ=255)                                                                                      gCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCaa                                     >  1:17161/1‑140 (MQ=255)                                                                                           gtgtATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGtt                                 >  1:963616/1‑139 (MQ=255)                                                                                           gtgtATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGtt                                 <  2:963616/139‑1 (MQ=255)                                                                                           gtgtATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTc                                >  1:1075708/1‑140 (MQ=255)                                                                                            tgtATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCg                               <  2:17161/140‑1 (MQ=255)                                                                                              tATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGCGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGa                             <  2:1075708/140‑1 (MQ=255)                                                                                                     gTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcga                      >  2:125919/1‑140 (MQ=255)                                                                                                       aGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcgacg                    <  2:371594/140‑1 (MQ=255)                                                                                                       aGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcgacg                    <  1:125919/140‑1 (MQ=255)                                                                                                             ccTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCa              >  2:140039/1‑140 (MQ=255)                                                                                                              cTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGa                             <  2:1032442/124‑1 (MQ=255)                                                                                                              cTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGa                             >  1:1032442/1‑124 (MQ=255)                                                                                                              cTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCaa             >  2:954142/1‑140 (MQ=255)                                                                                                                    cgcgCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATc       <  1:433851/140‑1 (MQ=255)                                                                                                                         cTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAg  >  1:1041040/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                   AAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAG  >  NC_000913/2703107‑2703366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑