Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,848,370	T→C	H291R (CAC→CGC)	hycC ←	formate hydrogenlyase subunit HycC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,848,370	0	T	C	100.0%	326.7 / NA	90	H291R (CAC→CGC)	hycC	formate hydrogenlyase subunit HycC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/46);  total (44/46)

TGAACAGGCTATGGTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGTGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTA  >  NC_000913/2848236‑2848505                                                                                                                                       |                                                                                                                                        tGAACAGGCTATGGTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTaa                                                                                                                                    >  1:1049216/1‑140 (MQ=255) tGAACAGGCTATGGTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTaa                                                                                                                                    >  2:865120/1‑140 (MQ=255)  gAACAGGCTATGGTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAg                                                                                                                                   <  2:1184936/140‑1 (MQ=255)        gCTATGGTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCcagca                                                                                                                             <  1:520550/140‑1 (MQ=255)        gCTATGGTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCcagca                                                                                                                             >  2:424562/1‑140 (MQ=255)          tATGGTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCTAGGGTGCGGTAAGCCAGCAgg                                                                                                                           <  2:1178549/140‑1 (MQ=255)          tATGGTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAgg                                                                                                                           >  2:1164947/1‑140 (MQ=255)              gTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCt                                                                                                                       >  2:147867/1‑140 (MQ=255)                  aGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAt                                                                                                                   >  2:926768/1‑140 (MQ=255)                  aGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAt                                                                                                                   >  2:936235/1‑140 (MQ=255)                  aGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAt                                                                                                                   >  1:1083110/1‑140 (MQ=255)                  aGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAt                                                                                                                   >  2:124760/1‑140 (MQ=255)                  aGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAt                                                                                                                   >  2:974081/1‑140 (MQ=255)                  aGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAt                                                                                                                   >  1:649416/1‑140 (MQ=255)                   gCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAtg                                                                                                                  <  1:936235/140‑1 (MQ=255)                      gATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAtgttg                                                                                                               <  1:926768/140‑1 (MQ=255)                      gATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAtgttg                                                                                                               >  2:99428/1‑140 (MQ=255)                      gATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAtgttg                                                                                                               <  1:1164947/140‑1 (MQ=255)                       aTGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCt                                                                                                                       <  2:1210535/131‑1 (MQ=255)                       aTGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAtgttgt                                                                                                              >  1:476077/1‑140 (MQ=255)                       aTGGTACAGACCACCGACCAGGCCAAGAGCAATAAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCt                                                                                                                       >  1:1210535/1‑131 (MQ=255)                        tGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTtgtg                                                                                                             <  1:99428/140‑1 (MQ=255)                           tACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTc                                                                                                          >  1:381494/1‑140 (MQ=255)                           tACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTc                                                                                                          >  2:330681/1‑140 (MQ=255)                             cAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTcca                                                                                                        <  2:476077/140‑1 (MQ=255)                               gACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTccacc                                                                                                      <  2:381494/140‑1 (MQ=255)                                 ccaccGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAg                                                                                                    >  2:593894/1‑140 (MQ=255)                                 ccaccGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAg                                                                                                    <  1:103290/140‑1 (MQ=255)                                  caccGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTcca                                                                                                        <  2:464880/135‑1 (MQ=255)                                  caccGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTcca                                                                                                        >  1:464880/1‑135 (MQ=255)                                  caccGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAgc                                                                                                   <  1:147867/140‑1 (MQ=255)                                  caccGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAgc                                                                                                   <  1:124760/140‑1 (MQ=255)                                    ccGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAgcgc                                                                                                 >  2:478536/1‑140 (MQ=255)                                    ccGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAgcgc                                                                                                 >  1:973758/1‑140 (MQ=255)                                    ccGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAgcgc                                                                                                 >  1:46276/1‑140 (MQ=255)                                     cGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAtgt                                                                                                                 >  1:716227/1‑123 (MQ=255)                                     cGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGAtgt                                                                                                                 <  2:716227/123‑1 (MQ=255)                                     cGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTcca                                                                                                        >  2:1152775/1‑132 (MQ=255)                                     cGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTcca                                                                                                        <  1:1152775/132‑1 (MQ=255)                                     cGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCa                                                                                                >  1:485062/1‑140 (MQ=255)                                     cGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCa                                                                                                >  2:431104/1‑140 (MQ=255)                                     cGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCa                                                                                                >  2:446240/1‑140 (MQ=255)                                     cGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCa                                                                                                >  1:131951/1‑140 (MQ=255)                                     cGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCa                                                                                                >  2:179327/1‑140 (MQ=255)                                       aCCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATa                                                                                              <  1:1055256/140‑1 (MQ=255)                                        ccAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATAc                                                                                             <  1:1199706/140‑1 (MQ=255)                                           ggCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGa                                                                                          <  1:871144/140‑1 (MQ=255)                                                 gagCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATAc                                                                                             <  2:762400/131‑1 (MQ=255)                                                 gagCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATAc                                                                                             >  1:762400/1‑131 (MQ=255)                                                  agCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGa                                                                                   <  2:69688/140‑1 (MQ=255)                                                   gCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGAc                                                                                  <  2:485062/140‑1 (MQ=255)                                                    cAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACa                                                                                 <  1:865120/140‑1 (MQ=255)                                                    cAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACa                                                                                 <  2:46276/140‑1 (MQ=255)                                                    cAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACa                                                                                 <  2:156088/140‑1 (MQ=255)                                                     aaTCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACaa                                                                                <  2:1049216/140‑1 (MQ=255)                                                      aTCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACaaa                                                                               >  2:562482/1‑140 (MQ=255)                                                      aTCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACaaa                                                                               >  1:12298/1‑140 (MQ=255)                                                        cAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAAcg                                                                             <  2:1083110/140‑1 (MQ=255)                                                         aGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAAcgc                                                                            <  1:974081/140‑1 (MQ=255)                                                           cgcCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCgg                                                                          <  1:701957/140‑1 (MQ=255)                                                            gcCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGt                                                                         >  2:29198/1‑140 (MQ=255)                                                                    ttCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGc                                                                 <  2:637736/140‑1 (MQ=255)                                                                     tCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGcc                                                                >  2:934181/1‑140 (MQ=255)                                                                       gAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGa                                                              <  2:973758/140‑1 (MQ=255)                                                                           gcgATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCAc                                                          <  2:661132/140‑1 (MQ=255)                                                                                   cGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAgcg                                                  <  2:968927/140‑1 (MQ=255)                                                                                    gTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAgcgc                                                 <  1:562482/140‑1 (MQ=255)                                                                                    gTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAgcgc                                                 <  1:179327/140‑1 (MQ=255)                                                                                        cGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATc                                             >  1:941771/1‑140 (MQ=255)                                                                                         gCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATcc                                            >  1:177530/1‑140 (MQ=255)                                                                                         gCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATcc                                            >  1:896865/1‑140 (MQ=255)                                                                                           cAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATcccc                                          <  1:478536/140‑1 (MQ=255)                                                                                               gcCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCccacc                                      >  2:856423/1‑140 (MQ=255)                                                                                                cccagccccagcAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCccacca                                     <  2:131951/140‑1 (MQ=255)                                                                                                 ccagccccagcAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCcaccac                                    <  1:446240/140‑1 (MQ=255)                                                                                                     ccccagcAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCAGCAGCAGCGCGa                                               >  1:864371/1‑125 (MQ=255)                                                                                                     ccccagcAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGa                                               <  2:864371/125‑1 (MQ=255)                                                                                                      cccagcAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCgg                               <  2:976554/140‑1 (MQ=255)                                                                                                      cccagcAGGATGATGCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCgg                               <  1:593894/140‑1 (MQ=255)                                                                                                               atgatgCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTAcc                      >  2:358033/1‑140 (MQ=255)                                                                                                               atgatgCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTAcc                      >  2:983379/1‑140 (MQ=255)                                                                                                                tgatgCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCg                     >  1:250822/1‑140 (MQ=255)                                                                                                                   tgCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGccc                  <  1:358033/140‑1 (MQ=255)                                                                                                                    gCCGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCca                 >  1:292599/1‑140 (MQ=255)                                                                                                                      cGATATTTTCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCcagc               >  1:576256/1‑140 (MQ=255)                                                                                                                           ttttCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGa          <  1:29198/140‑1 (MQ=255)                                                                                                                           ttttCCAGGGTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGa          <  2:576256/140‑1 (MQ=255)                                                                                                                                   ggTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGAc         <  1:156341/133‑1 (MQ=255)                                                                                                                                   ggTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGAc         >  2:156341/1‑133 (MQ=255)                                                                                                                                   ggTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTa  <  1:934181/140‑1 (MQ=255)                                                                                                                                   ggTGCGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTa  >  1:1148876/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TGAACAGGCTATGGTTAAGCAGATGGTACAGACCACCGACCAGGCCAAGAGCAATCAGCGCCGGTTGTTCGAGCGCGATACCCGTTACGCCAGCGCCCAGCCCCAGCAGGATGATGCCGATATTTTCCAGGGTGTGGTAAGCCAGCAGGCGCTGGATGTTGTGCTCCACCAGCGCATACAGACCACCGACAAACGCGGTGATCATGCCGAGCACCAGCAGCGCGATCCCCCACCACAGCGGTGCATTACCGCCCAGCAGTGACAGGGTTA  >  NC_000913/2848236‑2848505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑