Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,856,646	T→C	K54R (AAA→AGA)	ygbA ←	protein YgbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,856,646	0	T	C	100.0%	260.0 / NA	72	K54R (AAA→AGA)	ygbA	protein YgbA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/25);  total (47/25)

CATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTC  >  NC_000913/2856512‑2856782                                                                                                                                       |                                                                                                                                         cATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTctctct                                                                                                                                     <  2:1202510/140‑1 (MQ=255)       tgagatgaCGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTGACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCg                                                                                                                               <  1:217891/140‑1 (MQ=255)       tgagatgaCGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCg                                                                                                                               >  1:1216822/1‑140 (MQ=255)           atgaCGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAaca                                                                                                                           <  1:1014461/140‑1 (MQ=255)           atgaCGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAaca                                                                                                                           <  1:953922/140‑1 (MQ=255)               cGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGcc                                                                                                                                >  1:427673/1‑131 (MQ=255)               cGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGcc                                                                                                                                <  2:427673/131‑1 (MQ=255)                    ggTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACGCATTTAt                                                                                                                  <  2:518990/140‑1 (MQ=255)                     gTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATc                                                                                                                 >  2:215260/1‑140 (MQ=255)                      ttAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATcc                                                                                                                >  2:1015078/1‑140 (MQ=255)                      ttAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATcc                                                                                                                >  1:659664/1‑140 (MQ=255)                              ggATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCtt                                                                                                        >  1:397972/1‑140 (MQ=255)                              ggATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACACTTATCCAGCCGcct                                                                                                        >  2:404775/1‑138 (MQ=255)                                   aCGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTgcg                                                                                                   <  1:1015078/140‑1 (MQ=255)                                     gCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGcgcg                                                                                                 >  2:453484/1‑140 (MQ=255)                                      ccACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGt                                                                                                <  1:335266/140‑1 (MQ=255)                                       cacaGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTa                                                                                               >  1:760271/1‑140 (MQ=255)                                        acaGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaa                                                                                              <  1:779304/140‑1 (MQ=255)                                           gCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaaaca                                                                                           <  2:1216822/140‑1 (MQ=255)                                               tctcGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAaacaaaca                                                                                       >  1:232157/1‑140 (MQ=255)                                                    gcccggcccAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTgcg                                                                                                   >  1:1124416/1‑123 (MQ=255)                                                    gcccggcccAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTgcg                                                                                                   <  2:1124416/123‑1 (MQ=255)                                                     cccggcccAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTgcg                                                                                                   >  2:25728/1‑122 (MQ=255)                                                     cccggcccAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTgcg                                                                                                   <  1:25728/122‑1 (MQ=255)                                                         gcccAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTa                                                                             >  2:346811/1‑140 (MQ=255)                                                         gcccAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTa                                                                             >  2:1045487/1‑140 (MQ=255)                                                               cGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaaacaaa                                                                                         >  1:1076631/1‑122 (MQ=255)                                                               cGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaaacaaa                                                                                         >  1:1198321/1‑122 (MQ=255)                                                               cGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaaacaaa                                                                                         <  2:1076631/122‑1 (MQ=255)                                                               cGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaaacaaa                                                                                         <  2:1198321/122‑1 (MQ=255)                                                                    aaTCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATgctccggct                                                                  >  1:162683/1‑140 (MQ=255)                                                                    aaTCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATgctccggct                                                                  >  1:912988/1‑140 (MQ=255)                                                                    aaTCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATgctccggct                                                                  <  1:325122/140‑1 (MQ=255)                                                                       cTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATgctccggctccg                                                               >  2:255470/1‑140 (MQ=255)                                                                          tttCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTg                                                            >  1:828802/1‑140 (MQ=255)                                                                          tttCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTg                                                            >  2:153208/1‑140 (MQ=255)                                                                           ttCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGa                                                           <  2:1118570/140‑1 (MQ=255)                                                                                    tCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCgg                                                  >  1:770207/1‑140 (MQ=255)                                                                                    tCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCgg                                                  >  2:648213/1‑140 (MQ=255)                                                                                       cGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCa                                               <  2:125882/140‑1 (MQ=255)                                                                                            tGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCg                                          >  2:1189795/1‑140 (MQ=255)                                                                                            tGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCg                                          >  2:146983/1‑140 (MQ=255)                                                                                             gCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         <  1:951931/140‑1 (MQ=255)                                                                                             gCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         <  1:453484/140‑1 (MQ=255)                                                                                             gCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         <  2:589193/140‑1 (MQ=255)                                                                                             gCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         <  2:770207/140‑1 (MQ=255)                                                                                                    ggTAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATaa                                  >  1:1112667/1‑140 (MQ=255)                                                                                                      tAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         >  1:427873/1‑131 (MQ=255)                                                                                                      tAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         <  2:427873/131‑1 (MQ=255)                                                                                                      tAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAg                                >  1:999226/1‑140 (MQ=255)                                                                                                      tAACAGTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAg                                >  1:379500/1‑140 (MQ=255)                                                                                                           gTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCg                                          >  2:953736/1‑125 (MQ=255)                                                                                                           gTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCg                                          <  1:953736/125‑1 (MQ=255)                                                                                                           gTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         <  2:123282/126‑1 (MQ=255)                                                                                                           gTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         >  1:123282/1‑126 (MQ=255)                                                                                                           gTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAgatcga                           >  1:184552/1‑140 (MQ=255)                                                                                                           gTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAgatcga                           >  2:1234170/1‑140 (MQ=255)                                                                                                           gTGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAgatcga                           >  2:278431/1‑140 (MQ=255)                                                                                                            tGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         <  2:357770/125‑1 (MQ=255)                                                                                                            tGCACCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGc                                         >  1:357770/1‑125 (MQ=255)                                                                                                               aCCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCAt                       >  2:26474/1‑140 (MQ=255)                                                                                                               aCCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCAt                       >  2:1207653/1‑140 (MQ=255)                                                                                                               aCCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCAt                       >  1:190816/1‑140 (MQ=255)                                                                                                               aCCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCAt                       >  2:161949/1‑140 (MQ=255)                                                                                                               aCCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCAt                       >  1:1218983/1‑140 (MQ=255)                                                                                                               aCCGGACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCAt                       >  2:67118/1‑140 (MQ=255)                                                                                                                   gACACTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCAttttt                   >  1:401606/1‑140 (MQ=255)                                                                                                                      acTGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTa                <  2:828802/140‑1 (MQ=255)                                                                                                                        tGCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAAt              >  2:619774/1‑140 (MQ=255)                                                                                                                         gCTTACAGGCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATc             >  1:463768/1‑140 (MQ=255)                                                                                                                                ggCTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCA‑TTTTTTAATCGTCAGttt     >  2:1226264/1‑140 (MQ=255)                                                                                                                                  cTGGTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGtttt    >  1:1131802/1‑140 (MQ=255)                                                                                                                                    ggTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTc  >  1:1201130/1‑140 (MQ=255)                                                                                                                                    ggTCTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTc  >  1:1105834/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         CATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTCGTAATGCTCCGGCTCCGCTGACGCCTGCGGGCATTTCGCTTGATAAAGATCGATCATTTTTTTAATCGTCAGTTTTTC  >  NC_000913/2856512‑2856782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑