Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,080,867	T→C	T257A (ACC→GCC)	tktA ←	transketolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,080,867	0	T	C	100.0%	178.3 / NA	49	T257A (ACC→GCC)	tktA	transketolase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/18);  total (31/18)

CCTGGCCTGCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCG  >  NC_000913/3080732‑3080996                                                                                                                                        |                                                                                                                                  cctggcctgCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCg                                                                                                                               >  2:456899/1‑140 (MQ=255)     gcctgCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCtt                                                                                                                           >  1:336252/1‑140 (MQ=255)     gcctgCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCtt                                                                                                                           >  1:863986/1‑140 (MQ=255)      cctgCTTCTTTCGCATCCCACTGAGCATAGCTTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGCCGCCCAGCGGCGCACCGTGGGAGCCGTGGGCACCGGCttt                                                                                                                          >  2:202739/1‑140 (MQ=255)      cctgCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCttt                                                                                                                          >  2:958580/1‑140 (MQ=255)               ttCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTCCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGa                                                                                                                 >  1:1061335/1‑140 (MQ=255)                    aTCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGaaccga                                                                                                            <  2:863986/140‑1 (MQ=255)                              gCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCgatgat                                                                                                  >  1:563801/1‑140 (MQ=255)                                aTAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATgg                                                                                                >  2:969504/1‑140 (MQ=255)                                aTAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATgg                                                                                                >  1:740713/1‑140 (MQ=255)                                  aGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGtt                                                                                              >  1:771383/1‑140 (MQ=255)                                   gATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGttt                                                                                             >  1:238740/1‑140 (MQ=255)                                       tCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTAAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGca                                                                                         >  2:1094901/1‑140 (MQ=255)                                         aGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGcaca                                                                                       >  1:935271/1‑140 (MQ=255)                                             gggATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcag                                                                                   >  1:688626/1‑140 (MQ=255)                                             gggATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTCCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcag                                                                                   <  1:742323/140‑1 (MQ=255)                                               gATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcagca                                                                                 <  2:238740/140‑1 (MQ=255)                                               gATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcagca                                                                                 >  2:247507/1‑140 (MQ=255)                                               gATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATcagca                                                                                 <  2:336252/140‑1 (MQ=255)                                                     gAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAg                                                                           >  1:885565/1‑140 (MQ=255)                                                     gAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAg                                                                           <  1:969504/140‑1 (MQ=255)                                                     gAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAg                                                                           <  1:113716/140‑1 (MQ=255)                                                         ggCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGttt                                                                       >  2:203138/1‑140 (MQ=255)                                                         ggCGCATATTTCCAGCCCAGTTGTCCGCGGGTCAGGGCAA‑TTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTg                                                                      >  1:908891/1‑140 (MQ=255)                                                           cgcATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGt                                                                         >  1:422019/1‑136 (MQ=255)                                                           cgcATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGt                                                                         <  2:422019/136‑1 (MQ=255)                                                             cATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTgtca                                                                   >  1:414673/1‑140 (MQ=255)                                                               tatTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTgtcagt                                                                 <  2:414673/140‑1 (MQ=255)                                                                     cAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgc                                                           >  1:192083/1‑140 (MQ=255)                                                                      aGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcg                                                          <  2:740713/140‑1 (MQ=255)                                                                        cccAGTTGTCCGCAGGTCAGGGCAA‑TTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgc                                                       <  2:908891/140‑1 (MQ=255)                                                                         ccAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgc                                                       <  2:192083/140‑1 (MQ=255)                                                                         ccAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgc                                                       <  2:885565/140‑1 (MQ=255)                                                                            gttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGct                                                    >  2:1126897/1‑140 (MQ=255)                                                                            gttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGct                                                    >  1:531926/1‑140 (MQ=255)                                                                             ttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGctt                                                   >  2:396139/1‑140 (MQ=255)                                                                             ttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGctt                                                   >  1:1032499/1‑140 (MQ=255)                                                                               gttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGcttct                                                 <  1:203138/140‑1 (MQ=255)                                                                                  cgcgGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTtcttct                                              >  2:1164706/1‑140 (MQ=255)                                                                                       gTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgcgc                                                     <  2:789552/128‑1 (MQ=255)                                                                                       gTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTgcgcgcgc                                                     >  1:789552/1‑128 (MQ=255)                                                                                                 tttCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATa                               >  2:931379/1‑140 (MQ=255)                                                                                                             ccAGCGGCGCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCa                   <  1:958580/140‑1 (MQ=255)                                                                                                                 cggcgCACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGAc               >  2:1168516/1‑140 (MQ=255)                                                                                                                    cgcACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGt            >  1:603832/1‑140 (MQ=255)                                                                                                                      cACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCg          >  2:1045369/1‑140 (MQ=255)                                                                                                                      cACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCg          >  1:1018022/1‑140 (MQ=255)                                                                                                                      cACCGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCg          >  1:401544/1‑140 (MQ=255)                                                                                                                        ccGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGAt        <  1:202739/140‑1 (MQ=255)                                                                                                                        ccGTGGGAGTCGTGGGCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGAt        <  1:1126897/140‑1 (MQ=255)                                                                                                                                     gggCACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTcgcg  <  1:46636/133‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  CCTGGCCTGCTTCTTTCGCATCCCACTGAGCATAGATTTCAGACGGGATTTCGAACGGCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCG  >  NC_000913/3080732‑3080996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑