Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,525	A→G	N132S (AAC→AGC)	yghT →	putative ATP‑binding protein YghT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,525	0	A	G	100.0%	200.1 / NA	55	N132S (AAC→AGC)	yghT	putative ATP‑binding protein YghT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

AGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAACC  >  NC_000913/3134391‑3134657                                                                                                                                       |                                                                                                                                     aGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTg                                                                                                                                 >  1:870879/1‑140 (MQ=255)       tGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAggtg                                                                                                                           <  2:870879/140‑1 (MQ=255)       tGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAggtg                                                                                                                           >  2:250270/1‑140 (MQ=255)        gTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAggtgg                                                                                                                          <  2:1139668/140‑1 (MQ=255)             ccGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGtt                                                                                                                     <  1:107231/140‑1 (MQ=255)                      aCTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAt                                                                                                            >  2:282131/1‑140 (MQ=255)                         gcgcTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGtt                                                                                                                     <  1:180753/128‑1 (MQ=255)                         gcgcTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGtt                                                                                                                     >  2:180753/1‑128 (MQ=255)                          cgcTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCGACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggct                                                                                                        >  2:190986/1‑140 (MQ=255)                          cgcTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggct                                                                                                        >  2:333834/1‑140 (MQ=255)                            cTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      <  2:896136/140‑1 (MQ=255)                             tttCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctggc                                                                                                     <  1:250270/140‑1 (MQ=255)                                    atatGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAg                                                                                              <  2:889956/140‑1 (MQ=255)                                      atGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCAACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGa                                                                                            >  2:729431/1‑140 (MQ=255)                                         cTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGCCCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCC‑CCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      >  2:663192/1‑126 (MQ=255)                                         cTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCC‑CCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      <  1:663192/126‑1 (MQ=255)                                           tGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTa                                                                                       >  2:376712/1‑140 (MQ=255)                                             ccTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      >  1:446752/1‑123 (MQ=255)                                             ccTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      <  2:446752/123‑1 (MQ=255)                                               tGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGc                                                                                   >  2:1026141/1‑140 (MQ=255)                                                 tcgtcgtcgCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGt                                                                                 >  2:806867/1‑140 (MQ=255)                                                  cgtcgtcgCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCg                                                                                  <  1:305170/138‑1 (MQ=255)                                                  cgtcgtcgCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCg                                                                                  >  2:305170/1‑138 (MQ=255)                                                   gtcgtcgCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGttt                                                                               <  1:876686/140‑1 (MQ=255)                                                        cgcgGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTg                                                                          >  2:200189/1‑140 (MQ=255)                                                          cgGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGa                                                                        <  2:822218/140‑1 (MQ=255)                                                           gggTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGAt                                                                       <  1:376712/140‑1 (MQ=255)                                                               tAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCg                                                                   <  2:170863/140‑1 (MQ=255)                                                               tAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCg                                                                   <  1:200189/140‑1 (MQ=255)                                                                  ttGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGc                                                                >  2:798476/1‑140 (MQ=255)                                                                    gTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCa                                                              <  1:1172316/140‑1 (MQ=255)                                                                    gTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCAACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCa                                                              <  1:729431/140‑1 (MQ=255)                                                                      tCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATa                                                            >  2:826409/1‑140 (MQ=255)                                                                       cTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATaa                                                           >  2:357698/1‑140 (MQ=255)                                                                          accgaccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                                                        >  2:354244/1‑140 (MQ=255)                                                                          accgaccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                                                        >  2:1037037/1‑140 (MQ=255)                                                                               ccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCa                                                              >  2:836614/1‑129 (MQ=255)                                                                               ccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCa                                                              <  1:836614/129‑1 (MQ=255)                                                                                       cTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGtcatc                                           <  1:826409/140‑1 (MQ=255)                                                                                                 aaTTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATg                                 >  1:1042480/1‑140 (MQ=255)                                                                                                   ttCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGtt                               <  1:1037037/140‑1 (MQ=255)                                                                                                       tGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGAcc                           <  2:317682/140‑1 (MQ=255)                                                                                                            cTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGaa                      >  2:420357/1‑140 (MQ=255)                                                                                                             ttACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAg                     <  1:1026141/140‑1 (MQ=255)                                                                                                              tACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGt                    >  2:538478/1‑140 (MQ=255)                                                                                                               aCGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGtt                   <  1:798476/140‑1 (MQ=255)                                                                                                                 gATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGc                 <  1:357698/140‑1 (MQ=255)                                                                                                                     gTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCtgt             <  1:333834/140‑1 (MQ=255)                                                                                                                           tgtTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGt       >  1:1082967/1‑140 (MQ=255)                                                                                                                            gtTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTa      <  2:1042480/140‑1 (MQ=255)                                                                                                                             ttCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGc                 >  2:1178444/1‑128 (MQ=255)                                                                                                                             ttCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGc                 <  1:1178444/128‑1 (MQ=255)                                                                                                                             ttCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTaa     >  1:557222/1‑140 (MQ=255)                                                                                                                             ttCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTaa     >  1:1181843/1‑140 (MQ=255)                                                                                                                               cccACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAAc   >  2:650227/1‑140 (MQ=255)                                                                                                                               cccACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAAc   >  1:400686/1‑140 (MQ=255)                                                                                                                                ccACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAAcc  <  1:806867/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     AGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAACC  >  NC_000913/3134391‑3134657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑