| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,847,452 | (C)5→4 | coding (1173/1320 nt) | uhpC ← | inner membrane protein sensing glucose‑6‑phosphate |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,847,448 | 0 | C | . | 97.6% | 165.9 / ‑2.8 | 41 | coding (1177/1320 nt) | uhpC | inner membrane protein sensing glucose‑6‑phosphate |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (23/17); total (23/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGC > NC_000913/3847309‑3847583 | gCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGc < 2:718792/140‑1 (MQ=255) ttCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGccc > 1:836872/1‑140 (MQ=255) cgcgcgGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGcccca < 1:812925/140‑2 (MQ=255) gcgcgGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGccccag < 1:94173/140‑3 (MQ=255) cgcgGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGccccaga > 1:969193/1‑137 (MQ=255) cgcgGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGccccaga < 2:705692/140‑4 (MQ=255) cgcgGTGTATGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGccccaga > 1:372954/1‑137 (MQ=255) tgtCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGc < 2:836872/140‑1 (MQ=255) ttCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCg > 1:228114/1‑140 (MQ=255) ttCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCg > 2:953599/1‑140 (MQ=255) ttCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCg > 1:855771/1‑140 (MQ=255) cAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGAc > 1:141548/1‑140 (MQ=255) aGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAAcc < 2:228114/140‑1 (MQ=255) gTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTcg > 1:1072939/1‑140 (MQ=255) tAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATACCCACAAAAAATCCGCCCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGCCCAACCAGCAAGCGAC‑CCCCCAGATAAGCAAACAAGCCGACAAACCCCGTcgc < 2:514194/140‑1 (MQ=255) agcagTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCgccgcc < 1:953599/140‑1 (MQ=255) tGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTgc > 2:42357/1‑140 (MQ=255) tGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTgc > 2:566778/1‑140 (MQ=255) gCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTgcc > 1:278200/1‑140 (MQ=255) cGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTgccg > 2:398625/1‑140 (MQ=255) cGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTgccg > 2:8024/1‑140 (MQ=255) cGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTgccg > 1:1042174/1‑140 (MQ=255) aaaTCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCct > 1:356428/1‑140 (MQ=255) tCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCtt < 2:608581/140‑1 (MQ=255) tCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCtt < 2:448915/140‑1 (MQ=255) tCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCtt < 2:420088/140‑1 (MQ=255) tCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCtt < 2:372954/140‑1 (MQ=255) ccGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTtg < 1:42357/140‑1 (MQ=255) gcgATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGaa < 1:8024/140‑1 (MQ=255) tAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACAct < 1:398625/140‑1 (MQ=255) aCCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTgccgcc > 2:9515/1‑140 (MQ=255) aaaaaaTCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCAt > 1:921845/1‑140 (MQ=255) aaaaaaTCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCAt > 2:411682/1‑140 (MQ=255) aaaaaaCCCGCCCCAGTGCCGGGTATCGAGTATTTTCGCCAGCGCCCACCCAGCAAGCGCCG‑CCCCAGATAAGAAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCAt < 2:1072939/140‑1 (MQ=255) aaaaTCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATAc < 1:345386/140‑1 (MQ=255) aaaaTCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATAc < 2:141548/140‑1 (MQ=255) aaaaCCCGCCCCAGTCCCAGGTATCGAGTACTTCCGCCAGCGGCCACCCAGCAAGCG‑CCCCCCCAGATAACCAAACAAGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATAc < 2:855771/140‑1 (MQ=255) aTCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGa > 2:449106/1‑140 (MQ=255) cTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAAc < 1:449106/140‑1 (MQ=255) cTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAAc < 2:747321/140‑1 (MQ=255) ccAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTgg > 2:39599/1‑140 (MQ=255) tCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAg > 2:1035402/1‑140 (MQ=255) aGTACTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACa > 1:520658/1‑140 (MQ=255) aCTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAg > 1:908215/1‑134 (MQ=255) aCTTTCGCCAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAg < 2:908215/134‑1 (MQ=255) ccAGCGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCaa > 1:658912/1‑140 (MQ=255) cggCGGCCACCCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAAt < 2:278200/138‑1 (MQ=255) cGGCCAACCAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGt > 1:423581/1‑140 (MQ=255) ccAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGaagaag < 1:9515/140‑1 (MQ=255) ccAGCAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGaagaag < 1:39599/140‑1 (MQ=255) cAAGCGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAgg > 1:863513/1‑140 (MQ=255) cGACG‑CCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGc < 2:921845/140‑1 (MQ=255) cGACG‑CCCCAGATAAAAAAAAAAGCCGACAAACCCCGTCGCGGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCTCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGc < 2:969193/140‑1 (MQ=255) cgcccccccAAATAAGAAAACAAGCCGACAACCCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGc < 2:356428/136‑1 (MQ=255) | GCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGC > NC_000913/3847309‑3847583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |